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过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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Improvement in Resistance Switching of SiC-Based Nonvolatile Memory by Solution-Deposited HfO <sub/><i>x</i> </sub> Thin Film
摘要： In this study, we implemented a resistance change memory (ReRAM) device using a SiC layer with excellent physical properties. We fabricated devices composed of Ti/SiC/Pt and Ti/HfOx/SiC/Pt structures and investigated their memory characteristics. The Ti/SiC/Pt ReRAM devices exhibited stable bipolar resistive switching characteristics but had a relatively small memory window, whereas the Ti/HfOx/SiC/Pt ReRAM devices had a large memory window and low operating voltage. In addition, the Ti/HfOx/SiC/Pt ReRAM devices exhibited stable endurance characteristics over 500 cycles and excellent retention characteristics at room temperature and high temperatures for 1 × 104 s. Further, the Ti/HfOx/SiC/Pt ReRAM devices exhibited multi-level conduction states by modulating the reset stop voltage, and each resistance level had excellent endurance characteristics. The average transmittance of the HfOx/SiC bilayer in visible light was 87%. Such a high value indicates that the HfOx/SiC bilayer fabricated by the stacking method is expected to be a suitable material for highly reliable nonvolatile memory and transparent electronic devices, even in harsh environments.
关键词： Bilayer,ReRAM,BRS,Silicon Carbide,Multi-Level Cell (MLC)
更新于2025-09-04 15:30:14
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Deep-red fluorogenic probe for rapid detection of nitric oxide in Parkinson’s disease models
摘要： Nitric oxide (NO), as one of important gaseous signaling molecule in human body, has been associated with a variety of physiologic processes. However, excessive production of NO has been profoundly implicated in the pathogenesis of neurodegenerative disorders in particular, Parkinson's disease (PD). Therefore, accurate and facile detection of NO is of great significance for investigating its functions in PD, and the subsequent diagnosis and/or treatment. Herein, we developed a deep-red fluorogenic probe (BT-NH), with high sensitivity and good selectivity to detect NO, which was successfully used to visualize exogenous/endogenous NO level in living cells, and further applied to in vitro and in vivo PD models.
关键词： Parkinson’s disease,Drosophila,live cell imaging,nitric oxide,fluorogenic probe
更新于2025-09-04 15:30:14
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Dynamic Microfluidic Cytometry for Single-Cell Cellomics: High-throughput Probing Single-Cell-Resolution Signaling
摘要： Cell signaling is a fast dynamic and complex process, which controls a variety of critical physiological functions. Methods to investigate such dynamic information, however, suffer from limited throughput in single cells level and a lack of precise fluid manipulation. Herein, we present a new strategy, termed dynamic microfluidic cytometry (DMC), for high throughput probing GPCR signaling in single cells resolution (single-cell cellomics analysis) by creatively applied cyclical cell trapping, stimulating and releasing automatically. Dose-response curves and EC50 values for HeLa cells treated with adenosine triphosphate (ATP), histamine (HA) and acetylcholine chloride (ACH) were successfully obtained in single-cells level. High throughput single-cells dynamic signaling were further implemented by sequential or simultaneous stimulation, which revealed that different mechanisms were working in triggering intracellular calcium releasing. In addition, simultaneous stimulating to two different types of cells, HeLa and NIH-3T3 cells, was also successfully realized, which was crucial for online comparison dynamic signaling of different types of cells. We believe that the proposed DMC provides a versatile means for high throughput probing single cell dynamic signaling, which is potentially useful in chemical biology, cell biology and pharmacology.
关键词： dynamic signaling,cytometry,microfluidics,single-cell cellomics
更新于2025-09-04 15:30:14
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Hypoxia-Triggered Transforming Immunomodulator for Cancer Immunotherapy via Photodynamically Enhanced Antigen Presentation of Dendritic Cell
摘要： A key factor for successful cancer immunotherapy (CIT) is the extent of antigen presentation by dendritic cells (DCs) that phagocytize tumor-associated antigens (TAA) in the tumor site and migrate to tumor draining lymph nodes (TDLN), for the activation of T cells. Although various types of adjuvant delivery have been studied to enhance the activity of the DCs, poor delivery efficiency and depleted population of tumor infiltrating DCs have limited the efficacy of CIT. Herein, we report a hypoxia-responsive mesoporous silica nanocarrier (denoted as CAGE) for an enhanced CIT assisted by photodynamic therapy (PDT). In this study, CAGE was designed as a hypoxia-responsive transforming carrier to improve the intracellular uptake of nanocarriers and the delivery of adjuvants to DCs. Furthermore, PDT was exploited for the generation of immunogenic debris and recruitment of DCs in a tumor site, followed by enhanced antigen presentation. Finally, a significant inhibition of tumor growth was observed in vivo, signifying that the PDT would be a promising solution for DC-based immunotherapy.
关键词： combinatorial immunotherapy,hypoxia-responsive drug delivery,tumor-associated antigen,dendritic cell modulation,photodynamic therapy
更新于2025-09-04 15:30:14
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Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
摘要： We describe here a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and machine learning. Identification of lymphocyte subtypes is important for the study of immunology as well as diagnosis and treatment of various diseases. Currently, standard methods for classifying lymphocyte types rely on labeling specific membrane proteins via antigen-antibody reactions. However, these labeling techniques carry the potential risks of altering cellular functions. The protocol described here overcomes these challenges by exploiting intrinsic optical contrasts measured by 3D quantitative phase imaging and a machine learning algorithm. Measurement of 3D refractive index (RI) tomograms of lymphocytes provides quantitative information about 3D morphology and phenotypes of individual cells. The biophysical parameters extracted from the measured 3D RI tomograms are then quantitatively analyzed with a machine learning algorithm, enabling label-free identification of lymphocyte types at a single-cell level. We measure the 3D RI tomograms of B, CD4+ T, and CD8+ T lymphocytes and identified their cell types with over 80% accuracy. In this protocol, we describe the detailed steps for lymphocyte isolation, 3D quantitative phase imaging, and machine learning for identifying lymphocyte types.
关键词： lymphocyte identification,machine learning,holotomography,immune cell,immunology,Immunology and Infection,Quantitative phase imaging,optical diffraction tomography,holographic microscopy,label-free imaging
更新于2025-09-04 15:30:14
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Cell-Penetrating Peptides Transport Noncovalently Linked Thermally Activated Delayed Fluorescence Nanoparticles for Time-Resolved Luminescence Imaging
摘要： Luminescent probes and nanoparticles (NPs) with long excited state lifetimes are essential for time-resolved biological imaging. Generally, cell membranes are physiological barriers that could prevent the uptake of many unnatural compounds. It is still a big challenge to prepare biocompatible imaging agents with high cytomembrane permeability, especially for nonmetallic NPs with long-lived luminescence. Herein, an amphiphilic cell-penetrating peptide, F6G6(rR)3R2, was designed to transport hydrophobic fluorophores across cellular barriers. Three classical thermally activated delayed fluorescence (TADF) molecules, 4CzIPN, NAI-DPAC, and BTZ-DMAC, could self-assemble into well-dispersed NPs with F6G6(rR)3R2 in aqueous solution. These NPs showed low cytotoxicity and could penetrate membranes easily. Moreover, long-lived TADF enabled them to be used in time-resolved luminescence imaging in oxygenic environments. These findings greatly expanded the applications of cell-penetrating peptides for delivery of molecules and NPs by only noncovalent interactions, which were more flexible and easier than covalent modifications.
关键词： cell-penetrating peptide,Luminescent probes,time-resolved biological imaging,nanoparticles,noncovalent interactions,thermally activated delayed fluorescence
更新于2025-09-04 15:30:14
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Modeling Diffusion of Impurities in Molybdenum Thin Films as a Function of Substrate Temperature
摘要： Alkali ions are key to improving the performance of polycrystalline Cu(In,Ga)Se2 (CIGS) solar cells. Typically, a soda–lime glass substrate acts as an intrinsic source for many alkali ions. For these ions to reach the CIGS layer, diffusion through a metallic back contact is necessary. Typically deposited via sputtering, the morphology of this metallic back contact is dependent on multiple including substrate temperature. By deposition parameters, preparing ?lms with varying deposition parameters, and utilizing both, multiple material characterizations (X-ray diffraction, scanning electron microscopy, and secondary ion mass spectrometry) and numerical modeling, we demonstrate here that effective paths of diffusion are just as important as diffusion rate for the alkali ions. As the substrate temperature increases, the mechanism that hinders the ability for alkali ions to diffuse switches effectively from diffusion rate to effective path of diffusion. It is shown that the lack of viable diffusion paths at substrate temperatures above 100 °C becomes the dominant factor for the transport of alkali ions.
关键词： solar cell,impurities,Back contact,modeling,diffusion,molybdenum,Cu(In,Ga)Se2 (CIGS)
更新于2025-09-04 15:30:14
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SAR Ground Moving Target Imaging Based on a New Range Model Using a Modified Keystone Transform
摘要： Imaging of a ground moving target (GMT) with synthetic aperture radar (SAR) is a challenging task, particularly when the Doppler ambiguity happens. This paper proposes a novel GMT range model and a novel Doppler ambiguity-tolerated GMT imaging algorithm based on modified keystone transform. In the proposed GMT range model, the range from radar antenna to the scattering center on the target is divided into two parts: the range from radar antenna to the target centroid and the projection length on the light of sight from the target centroid to the scattering center. Based on the new range model, a Doppler ambiguity-tolerated range cell migration correction (RCMC) method is proposed, in which keystone transform is modified to realize the differential RCMC by interpolation with nonzero phase sinc kernel. Finally, the GMT image is obtained in the range-Doppler domain by matched filtering in the slow-time domain and is restored into the 2-D space domain. The effectiveness of our proposed model and imaging method is demonstrated by both simulated and real airborne SAR data.
关键词： Ground moving target imaging (GMTIm),keystone transform,range cell migration correction (RCMC),range model,synthetic aperture radar (SAR)
更新于2025-09-04 15:30:14
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Suppression of poisoning of photocathode catalysts in photoelectrochemical cells for highly stable sunlight-driven overall water splitting
摘要： A photoelectrochemical (PEC) cell composed of two semiconductor electrodes, a photocathode, and a photoanode is a potentially effective means of obtaining hydrogen through spontaneous overall water splitting under light irradiation. However, the long-term stability (that is, operation for more than one day) of a PEC cell has not yet been demonstrated. In addition to the corrosion of both photoelectrodes, the gradual migration of heavy metal cations from the photoanode into the electrolyte can also result in degradation of the cell by contamination of the photocathode surface. In the present work, BiVO4-based photoanodes were used in conjunction with two different modifications: dispersion of a chelating resin in the electrolyte and coating of the photoanode surface with an anion-conducting ionomer. The chelating resin was found to capture Bi3+ cations in the electrolyte before they became deposited on the cathode surface. Consequently, a PEC cell incorporating a BiVO4-based photoanode and a (ZnSe)0.85(CuIn0.7Ga0.3Se2)0.15-based photocathode showed stable overall water splitting over a span of two days under simulated sunlight. To the best of our knowledge, this represents the longest period over which stable PEC cell performance has been established. A considerable decrease in the performance of the BiVO4-based photoanode was still observed due to the continuous dissolution of Bi species, but surface coating of the photoanode with an anion-conducting ionomer prevented the movement of Bi3+ ions into the electrolyte because of the selective conduction of ions. The coating also served as a protective layer that improved the durability of the photoanode. This study therefore suggests a simple yet effective method for the construction of stable PEC cells using semiconductor photoelectrodes.
关键词： chelating resin,photoanode,stability,BiVO4,anion-conducting ionomer,water splitting,photoelectrochemical cell,photocathode
更新于2025-09-04 15:30:14
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Quantifying single-cell secretion in real time using resonant hyperspectral imaging
摘要： Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.
关键词： single-cell analysis,photonic biosensing,photonic crystal,label-free
更新于2025-09-04 15:30:14