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Luminescent rhenium(I) carbonyl complex with redox noninnocent ONS donor azo-phenol ligand: Synthesis, X-ray structure, photophysical properties and live cell imaging
摘要： Herein, we have synthesized a new fluorescent rhenium(I) carbonyl complex 1, bearing {Re(CO)3}+ core with ONS donor thioether containing azo-phenol redox noninnocent ligand. The distorted octahedral geometry of the complex is confirmed by single crystal X-ray diffraction method. Cyclic voltammogram in acetonitrile exhibits irreversible oxidation peak (Epa = 1.36 V) along with quasi-reversible reduction peak at E1/2 = -0.92 V (ΔE = 210 mV). The complex exhibits low energy emission band at 525 nm with high emission quantum yield (Φ = 0.115). Cytotoxicity of the complex is studied by MTT method with human breast cancer cell lines (MCF-7) and IC50 value is found to be 23.6 μM. In presence of the complex (10 μM) a bright green fluorescence image of MCF-7 cell lines is observed under fluorescence microscope.
关键词： Electrochemistry,ONS donor ligand,MTT assay,Rhenium(I) carbonyl complex,Live cell imaging
更新于2025-11-19 16:46:39
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Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation
摘要： A turn-on fluorometric method is described for selective and sensitive detection of cysteine (Cys). Gold nanoclusters (Au NCs) capped with glutathione (GSH) are used as a fluorescent probe. If Ce3+ ion are present, they will bind to the carboxy groups of the GSH-capped Au NC. This results in aggregation-induced emission enhancement (AIEE), best measured at excitation/emission wavelengths of 360/575 nm. On addition of Cys, which has less steric hindrance compared with GSH and higher affinity for Ce3+, it will bind to Ce3+ through the carboxyl group and link with Au NCs via Au-S bond. Hence, the AIEE is increased and Cys can be quantified via this effect with a linear response in the 0.4–120 μmol L?1 Cys concentration range and a detection limit of 0.15 μmol L?1.
关键词： Au NC,HepG2 cell imaging,Aggregation-induced emission enhancement
更新于2025-11-14 15:26:12
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[Methods in Molecular Biology] T-Cell Motility Volume 1930 (Methods and Protocols) || Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time
摘要： T-lymphocytes are the principle coordinators of the immune defense system and play a major role in the protection of our body against infections, intruders of non-self, and malignancies. To mount an immune response, T-cells need to be effectively employed to tissue sites of infection or in?ammation and establish contacts with antigen-presenting cells (APCs) or malignant cells. Understanding how T-cells navigate toward their recruitment sites would offer new therapeutic opportunities. Advancement in the hardware and software upgrades of microscopy technology has created several ef?cient and easy-to-operate live cell imaging platforms. In this protocol, we present a generalized and simple-to-follow protocol for live cell imaging of migrating T-cells, which can also be adopted to visualize real-time tracking of intracellular signaling events.
关键词： Advanced microscopy,Cell tracking,Live cell imaging,T-cell migration
更新于2025-09-23 15:23:52
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[IEEE 2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Hangzhou, China (2018.8.13-2018.8.17)] 2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Determination of Optimal Curing Conditions for Imaging Single Lung Cancer Cells by Atomic Force Acoustic Microscope
摘要： This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.
关键词： A549 cell,cell curing,Atomic force acoustic microscope,cured cell imaging
更新于2025-09-23 15:23:52
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One-pot green synthesis of highly luminescent silicon nanoparticles using Citrus limon (L.) and their applications in luminescent cell imaging and antimicrobial efficacy
摘要： Strongly luminescent and water dispersible silicon nanoparticles were synthesised by one-pot synthesis from venyl-trimethoxy-silane and (3-aminopropyl)-triethoxy-silane using both chemical and green reducing agents, sodium ascorbate and Citrus limon (L.) respectively. Unlike sodium ascorbate, the reduction using lemon extracts produces smaller nanoparticles with average size of 20 and 57 nm. The antimicrobial e?cacy of the nanoparticles is examined against various microorganisms and is noted that these particles possessed antibacterial property if used in concentrations above the threshold value of 1.5 μg/ml. In addition, these silicon nanoparticles emit intense blue-green emission which is successfully utilised for the luminescent cell imaging of human white blood cells and mice epithelial cells.
关键词： Luminescence,Citrus limon (L.),Silane,Silicon nanoparticle,Cell imaging.
更新于2025-09-23 15:23:52
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Novel rhodamine-based colorimetric and fluorescent sensor for the dual-channel detection of Cu2+ and Co2+/trivalent metal ions and its AIRE activities
摘要： A rhodamine hydrazone 1 bearing coumarin moiety was designed and prepared. Compound 1 exhibited high selectivity toward Co2+ and trivalent metal ions with fluorescence enhancement in CH3OH solution. However, 1 selectively responded to Al3+ in nearly pure H2O media and was further applied to monitor Al3+ in live cells. Moreover, 1 could also act as a colorimetric probe toward Cu2+ in either CH3OH or H2O solution. In addition, sensor 1 displayed aggregation-induced ratiometric emission (AIRE) activities in mixed H2O/CH3OH solution.
关键词： cell imaging,coumarin,fluorescent probe,Aggregation-induced ratiometric emission,metal ions,rhodamine
更新于2025-09-23 15:23:52
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Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells
摘要： Alveolar type II(ATII) cells in the peripheral human lung spontaneously differentiate towards alveolar type I(ATI) cells, thus enabling air-blood barrier formation. Here, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy are applied to study cell differentiation of freshly isolated ATII cells. The Raman spectra can successfully be correlated to gradual morphological and molecular changes during cell differentiation. Alveolar surfactant rich vesicles in ATII cells are identified based on phospholipid vibrations, while ATI-like cells are characterized by the absence of vesicular structures. Complementary, CARS microscopy allows for 3D visualization of lipid vesicles within ATII cells and their secretion, while hyperspectral CARS enables the distinction between cellular proteins and lipids according to their vibrational signatures. This study paves the path for further label-free investigations of lung cells and the role of the pulmonary surfactant, thus also providing a basis for rational development of future lung therapeutics.
关键词： confocal laser scanning microscopy,confocal Raman microscopy,pneumocyte type II differentiation,CARS microscopy,cell imaging
更新于2025-09-23 15:23:52
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[Methods in Molecular Biology] Autophagy Volume 1880 (Methods and Protocols) || Correlative Light and Electron Microscopy of Autophagosomes
摘要： Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.
关键词： Autophagosome,Phagophore,Electron tomography,Live-cell imaging,Serial sectioning
更新于2025-09-23 15:23:52
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A new benzimidazole-based selective and sensitive ‘on-off’ fluorescence chemosensor for Cu <sup>2+</sup> ions and application in cellular bioimaging
摘要： Two new twinborn benzimidazole derivates (L and A), which bonded pyridine via the ester space on the opposite and adjacent positions of the benzene ring of benzimidazole respectively, were designed and synthesized. Compound L displayed fluorescence quenching response only towards copper(II) ions (Cu2+) in acetonitrile solution with high selectivity and sensitivity. However, compound A presented 'on–off' fluorescence response towards a wide range of metal ions to different degrees and did not have selectivity. Furthermore, compound L formed a 1:1 complex with Cu2+ and the binding constant between sensor L and Cu2+ was high at 6.02 × 104 M?1. Job's plot, mass spectra, IR spectra, 1H‐NMR titration and density functional theory (DFT) calculations demonstrated the formation of a 1:1 complex between L and Cu2+. Chemosensor L displayed a low limit of detection (3.05 × 10?6 M) and fast response time (15 s) to Cu2+. The Stern–Volmer analysis illustrated that the fluorescence quenching agreed with the static quenching mode. In addition, the obvious difference of L within HepG2 cells in the presence and absence of Cu2+ indicated L had the recognition capability for Cu2+ in living cells.
关键词： fluorescence chemosensor,benzimidazole,cell imaging,copper ion
更新于2025-09-23 15:23:52
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Fluorescein derived Schiff base as fluorimetric zinc (II) sensor via ‘turn on’ response and its application in live cell imaging
摘要： A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction, which is utilized for selective sensing of Zn2+ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn2+ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV–Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods. The binding constant (Ka) for complexation and the limit of detection (LOD) of Zn2+ is calculated to be 2.86×104 M-1 and 1.59 μM, respectively. Further photophysical investigations including steady-state, time-resolved fluorescence analysis and spectral investigations including NMR (nuclear magnetic resonance), IR (infrared spectroscopy) suggest introduction of CHEF (chelation enhance fluorescence) with the suppression of C=N isomerization and PET (photo-induced electron transfer) mechanism for the strong fluorescent response towards Zn2+. Finally, the sensor L is successfully employed to monitor a real-time detection of Zn2+ by means of TLC (thin layer chromatography) based paper strip. The L is used in the cell imaging study using African green monkey kidney cells (Vero cells) for the determination of exogenous Zn2+ by Immunofluorescence Assay (IFA) process.
关键词： Schiff base,molecular logic gate,cell imaging,Zn sensor,PL and TCSPC,UV-Vis
更新于2025-09-23 15:23:52