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- 实验方案
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Dual-Color Plasmonic Nanosensor for Radiation Dosimetry
摘要： Radiation dosimeters are critical for accurately assessing the levels of radiation exposure of tumor sites and surrounding tissues and for optimizing therapeutic interventions as well as for monitoring environmental exposure. To fill the need for a simple, user-friendly and inexpensive dosimeter, we designed an innovative colorimetric nanosensor-based assay for detecting ionizing radiation. We show that hydroxyl radicals generated by ionizing radiation can be used to etch gold nanorods (AuNRs) and silver nanoprisms (AgNPRs), yielding reproducible color changes for radiation dose detection in the range of 50-2000 Rad, broad enough to cover doses used in hyperfractionated, conventional, and hypofractionated radiotherapy. This range of doses detected by this assay correlates with radiation induced DNA damage response in mammalian cells. Furthermore, this AuNR- and AgNPR-based sensing platform has been established in a paper format that can be readily adopted for a wide range of applications and translation.
关键词： Radiation dosimetry,gold nanorods,silver nanoprisms,dual-color nanosensor,colorimetric detection
更新于2025-09-23 15:19:57
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A colorimetric and fluorescent chemosensor for Hg <sup>2+</sup> based on a photochromic diarylethene with a quinoline unit
摘要： A new colorimetric and fluorescent 'on–off' chemosensor, 1O, based on a photochromic diarylethene with a quinoline unit was designed and synthesized. The chemosensor 1O demonstrated selective and sensitive detection of Hg2+ ions in the presence of other competitive metal ions in acetonitrile. The stoichiometric ratio of the sensor 1O for Hg2+ was determined to be 1 : 1, and the limit of detection of the probe 1O was calculated to be 56.3 nM for Hg2+. In addition, a molecular logic circuit with four inputs and one output was successfully constructed with UV/vis light and metal-responsive behavior. ESI-MS spectroscopy, Job's plot analysis, and 1H NMR titration experiments confirm the binding behavior between 1O and Hg2+.
关键词： quinoline,logic circuit,fluorescent,Hg2+,colorimetric,diarylethene,chemosensor,photochromic
更新于2025-09-19 17:15:36
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Spectrophotometric and RGB performances of a new tetraphenylcyclopenta-derived Schiff base for the quantification of cyanide ions
摘要： In this communication, a new cyanide (CN-) ion selective chromogenic receptor (L) was developed by reacting 2,4-dinitrophenylhydrazine with 2,3,4,5-tetraphenylcyclopenta-2,4-dienone. The sensing ability of receptor L was studied with different anions such as F-, Cl-, Br-, I-, OH-, CN-, SO4^2-, PO4^3-, CH3COO- and NO3- by visual test, UV-Vis spectrometry and smartphone based RGB tool in THF:H2O (6:4, v/v) media. The receptor L showed a strong absorption band at 412 nm due to the presence of donor and acceptor units that results in an intense ligand-to-ligand charge-transfer (LLCT). Upon interaction with CN-, the L showed a selective colour change from yellow to blue along with the formation of a new UV-Vis band at 615 nm. The absorption spectral studies indicates the formation of a complex between the L and CN- with an estimated association constant of 8.9×10^3 M^-1 and binding stoichiometry between them is 1:1. The binding interaction of L with CN- was examined using 1H NMR and density functional theory (DFT) calculations. The sensitivity of L is recognized by limit of detection (LOD) and it is as 1x10^-7 M which is lower than the WHO guidelines (0.02 ppm) in drinking water. The colorimetric change of L has been well integrated with a smartphone RGB color value to make it an analytical signal for real-time analysis of CN- with the detection limit down to 3.98x10^-6 M. The L detects CN- without any interference from other tested anions over a wide pH range 3-10. During measurements of absorbance and RGB values of all tested solutions were adjusted to pH 7.5 Besides, L was applied for the analysis of CN- content present in various eatable samples by spectrophotometry, test paper strips and RGB colour tool.
关键词： smartphone RGB,spectrophotometry,colorimetric sensor,cyanide detection,chromogenic receptor
更新于2025-09-19 17:15:36
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Rhodamine-assisted fluorescent strategy for the sensitive and selective in-field mapping of environmental pollutant Hg(II) with potential bioimaging
摘要： Mercury is a global pollutant that can damage human brain, central nervous system and endocrine moreover, other biological systems. Its real-time monitoring is a key challenge in the control of mercury emissions or pollution. In this study, a simple and efficient fluorescent sensor (PST) comprising of rhodamine B and 2-amino-5-bromopyridine was developed for the detection of environmental toxicant Hg2+. The newly synthesized sensor displayed a highly selective and sensitive 'turn-on' response by the addition of Hg2+ among miscellaneous metal cations (Ag+, Al3+, Ba2+, Ca2+, Cd2+, Fe3+, Fe2+, K+, Li+, Mg2+, Mn2+, Cu2+, Pb2+, Ni2+, Na+ and Zn2+) in CH3CN: H2O (8:2 v/v) system. The 1:1 binding stoichiometry, limit of detection (LOD) 0.63 μM along with binding constant of 1.04 × 105 M?1 was calculated for the complex PST-Hg2+. The reversible nature of the complex was confirmed in the presence of ethylenediaminetetraacetate (EDTA). The significant enhancement of fluorometric and absorption response was attributed to the spirolactam ring opening via photo-induced electron transfer (PET) of xanthene moiety. This spirolactam ring opening was further verified by density functional theory, 1H NMR and Fourier transform infrared spectroscopy (FT-IR). The real-time applicability of the sensor in environmental and biological fields was proved as detection kit on TLC strips and with the help of Laser confocal microscopy for live cell imaging by using HeLa cells. Conclusively, PST can be regarded as accurate 'two in one' kit for colorimetric recognition of Hg2+ in the environment and fluorescent cell imaging in live HeLa cells.
关键词： Rhodamine,High sensitivity,Colorimetric,Cell imaging,Infield,Toxic metal
更新于2025-09-19 17:15:36
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A large Stokes shift, sequential, colorimetric fluorescent probe for sensing Cu2+/S2- and its applications
摘要： Copper ions (Cu2+) and sulfide (S2?) are important markers in many physiologies and pathological processes. In this work, a new near-infrared fluorescent probe 1 for colorimetric and sequential detection of Cu2+/S2? was designed and developed. The probe showed a rapid (less than 1 min), highly selective and sensitive response toward copper ions. Notably, the probe could also be applied to detect S2? through reversible formation-separation of complex 1-Cu2+ and CuS with a large Stokes shift of 234 nm. The detection limit for Cu2+ and S2? was found to be 1.8 × 10-8 M and 1.5 × 10-8 M, respectively. Furthermore, the binding stoichiometry between 1 and Cu2+ was found to be 1:1, the binding mode was also demonstrated using density functional theory (DFT) calculations and contrastive compound research. In addition, the probe was successfully applied in real water samples assay for the detection of Cu2+, and the strip papers experiments also showed that probe 1 can be used to detect Cu2+ and S2?.
关键词： DFT calculations,Colorimetric,Near-infrared,Sulfide,Large Stokes shift,Copper ions
更新于2025-09-19 17:15:36
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Colorimetric Polymerase Chain Reaction Enabled by a Fast Light-Activated Substrate Chromogenic Detection Platform
摘要： Miniaturization of nucleic acid tests (NATs) into portable, inexpensive detection platforms may aid disease diagnosis in point-of-care (POC) settings. Colorimetric signals are ideal readouts for portable NATs, and it remains of high demand to develop color readouts that are simple, quantitative and versatile. Thus motivated, we report a Fast Light-Activated Substrate cHromogenic polymerase chain reaction (FLASH PCR) that uses DNA intercalating dyes (DIDs) to enable colorimetric nucleic acid detection and quantification. The FLASH system is established on our finding that DID-DNA intercalation can promote the rapid photooxidation of chromogenic substrates through light-induced production of singlet oxygen. Using this principle, we have successfully converted DID-based fluorescent PCR assays into colorimetric FLASH PCR. To demonstrate the practical applicability of FLASH PCR to POC diagnosis, we also fabricated two readout platforms, including a portable electronic FLASH reader and a paper-based FLASH strip. Using the FLASH reader, we were able to detect as low as 60 copies of DNA standards, a limit of detection (LOD) comparable with commercial quantitative PCR. The FLASH strip further enables the reader-free detection of PCR amplicons by converting the colorimetric signal into the visual measurement of distance as a readout. Finally, the practical applicability of the FLASH PCR was demonstrated by the detection and/or quantification of nucleic acid markers in diverse clinical and biological samples.
关键词： FLASH,Singlet Oxygen,Photooxidation,DNA Intercalating Dyes,Colorimetric,Nucleic Acid Tests,Paper-based Strip,Point-of-Care,Portable Electronic Reader,Polymerase Chain Reaction
更新于2025-09-19 17:13:59
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Orange‐Emissive Carbon Quantum Dots: Toward Application in Wound pH Monitoring Based on Colorimetric and Fluorescent Changing
摘要： Monitoring of wound pH is critical for interpreting wound status, because early identification of wound infection or nonhealing wounds is conducive to administration of therapies at the right time. Here, novel orange-emissive carbon quantum dots (O-CDs) are synthesized via microwave-assisted heating of 1,2,4-triaminobenzene and urea aqueous solution. The as-prepared O-CDs exhibit distinctive colorimetric response to pH changing, and also display pH-sensitive fluorescence. Benefiting from the response of O-CDs over a wound-relevant pH range (5–9), medical cotton cloth is selected to immobilize O-CDs through hydrogen bond interactions, the resultant O-CDs-coated cloth with emission at 560 nm shows a high response to pH variation in the range of 5–9 via both fluorescence and visible colorimetric changes. Moreover, the sensitivity of fluorescence to pH is capable of establishing an analytical mode for determining pH value. Further, the O-CDs-based pH indicator possesses not only superior biocompatibility and drug compatibility but also excellent resistance leachability and high reversibility. Importantly, the usage of O-CDs-coated cloth to detect pH is free from the interference of blood contamination and long-term storage, thus providing a valuable strategy for wound pH monitoring through visual response and quantitative determination.
关键词： fluorescent bandages,wound pH monitoring,carbon quantum dots,colorimetric response,dual-mode
更新于2025-09-19 17:13:59
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Smartphone coupled with a paper-based optode: Towards a selective cyanide detection
摘要： A low-cost on-paper sensor based on 5,10,15-tritolylcorrolatocobalt(III) triphenylphosphine, CoTTCorr(PPh3), was developed for cyanide detection in aqueous solutions. The sensor was coupled to a smartphone and used a home-written color intensity analysis software in order to record and interpret the colorimetric response. The detection of cyanide was possible down to 0.053 mg/L, an order of magnitude lower than the value of 0.5 mg/L set by the World Health Organization (WHO) for safe short-term exposure of cyanide in potable water. The colorimetric sensor had selectivity toward cyanide ions over the anions Cl-, Br, F-, NO2, SCN-, OAc-,ClO4- while the influence of NO3 ions on the sensor optical response towards cyanide was overcome by optimization of the ionophore/anion-exchanger ratio inside the sensing material. The best performance was obtained for the optode with an ionophore to exchanger ratio of 1:3. The optimized optodes were employed for quantification of cyanide content added to potable water and saliva.
关键词： 5,cyanide sensor,15-tritolyl corrolate Co(III) triphenylphosphine,colorimetric detection,10
更新于2025-09-19 17:13:59
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Highly Sensitive Detection of Salbutamol by ALP-mediated Plasmonic ELISA Based on Controlled Growth of AgNPs
摘要： Salbutamol (SAL), a short-acting β-2 adrenergic receptor agonist, is an undesirable addition to livestock farming and used to improve the growth rates. A direct competitive colorimetric immunoassay for SAL was developed. The alkaline phosphatase (ALP)-mediated controlled growth of the prereduction silver nanoparticles (AgNPs) was used to achieve the highly sensitive, colorimetric detection of SAL through the plasmonic ELISA. Click chemistry reaction was used to synthesize salbutamol biotin label (SAL-Bio) to achieve signal amplification and specific binding with antibody. ALP can efficiently hydrolyze sodium L-ascorbyl-2-phosphate and generate L-ascorbic acid, thereby reducing Ag+ and forming AgNPs. The plasmon resonance absorption signals of AgNPs were enhanced along with a distinct color change. The limit of detection of SAL can be decreased to 26.14 pg/mL. The method offers a good technique for the detection of many small molecular contaminants, such as pesticide residues and veterinary drug residues in food and edible water.
关键词： Salbutamol,alkaline phosphatase,colorimetric detection,direct competitive immunoassay,plasmonic ELISA,silver nanoparticles
更新于2025-09-19 17:13:59
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A novel “on-off” colorimetric sensor for glutathione based on peroxidase activity of montmorillonite loaded TiO2 functionalized by porphyrin precisely controlled by visible light
摘要： Well-dispersed nanocomposites based on Ca-montmorillonite decorated by porphyrin functionalized titanium dioxide (H2TCPP-TiO2-MMT) was synthesized by a facile strategy. H2TCPP-TiO2-MMT was demonstrated to possess the intrinsic peroxidase-like activity and could catalyze 3’,3’,5’,5’-tetramethylbenzidine (TMB) oxidized by H2O2 accompanied with the color changed from colorless to blue in a short time under visible light irradiating. The optimum experimental conditions, robustness, as well as Michaelis-Menten kinetics was investigated. The fluorescent probe assay indicated the good performance of H2TCPP-TiO2-MMT was attributed to the ·OH radicals from H2O2 decomposition, which was induced by the electron-hole pairs of H2TCPP-TiO2-MMT generated under visible light irradiating. Based on its peroxidase activity, a convenient colorimetric sensor for detecting glutathione (GSH) was proposed. The detection limit for GSH was 0.057 μM, with the linear detection range of 0.1-20 μM. Notably, besides the enhanced peroxidase-like activity, another merit for this sensor was that the reaction time can be controlled accurately by adjusting irradiation time compared with traditional colorimetric sensors, which would benefit the accuracy in detecting GSH. In addition, results of anti-interference and detecting GSH in human serum assays indicated that the H2TCPP-TiO2-MMT was predicted to be one of the promising materials for fast visual colorimetry.
关键词： Peroxidase,Montmorillonite,Porphyrin,Colorimetric assay
更新于2025-09-16 10:30:52