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oe1(光电查) - 科学论文

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出版时间
  • 2018
研究主题
  • metal object detection (MOD)
  • wireless power transfer (WPT)
  • auxiliary detection coil
  • Chest X-ray (CXR)
  • Computer-aided Diagnosis (CADx)
  • Early detection of tuberculosis
应用领域
  • Electrical Engineering and Automation
  • Optoelectronic Information Science and Engineering
机构单位
  • Shanghai Jiao Tong University
  • Bandung Institute of Technology (ITB)
1611 条数据
?? 中文（中国） ▾

  • Ultrathin Ti3C2 Nanosheets based “off-on” Fluorescent nanoprobe for Rapid and Sensitive Detection of HPV Infection

    摘要： MXenes as a new class of 2D materials have recently been widely applied in energy storage, electrocatalysis, sensors, adsorption, water purification, and so on, due to their tunable versatile properties. Herein, we demonstrate a simple, rapid and highly-sensitive sensing platform based on ultrathin two-dimensional MXene Ti3C2 nanosheets (Ti3C2 NSs) for selective analysis of Human papillomavirus (HPV), a major human pathogens and causative agents of cervical cancer. Ultrathin Ti3C2 NSs, obtained by exfoliating their layered HF-etched powder, exhibit high fluorescence quenching ability to dye-labeled single-stranded DNA (ssDNA) and different affinities for ssDNA and double-stranded DNA (dsDNA). Under the fluorescence quenching effect of Ti3C2 NSs, ssDNA probe (P) shows the minimal fluorescent emission. After the formation of duplex structure with its complementary target, ssDNA (T), the fluorescence intensity enhances evidently. Exonuclease III (Exo III) was used to improve the sensitivity by promoting more fluorescence enhancement. This magnified fluorescent sensor for HPV-18 detection shows a low detection limit of 100 pM and a high specificity. Furthermore, the developed DNA sensor can be employed to determine PCR amplified HPV-18 from cervical scrapes samples. It highlights ultrathin Ti3C2 NSs as a potential candidate for construction of fluorescence DNA biosensors with excellent performances.

    关键词： DNA hybridization,Ti3C2 nanosheets,HPV,Fluorescent detection

    更新于2025-11-19 16:46:39

  • Enzyme-free fluorometric assay for chloramphenicol based on double stirring bar-assisted dual signal amplification

    摘要： An enzyme-free fluorometric assay is described that accomplishes dual signal amplification by making use of a two stirring bars. Two Y-shaped DNA probes were designed and placed on the bars. When the target (with chloramphenicol as model analyte) is added, it triggers target recycling and simultaneously catalyzes hairpin assembly (CHA). A large fraction of DNA primers is released by the analyte from the bar to the supernatant and open hairpins with G-quadruplex DNA sequence. The G-quadruplex can specifically bind thioflavin T (ThT) to emit fluorescence (with excitation/emission maxima at 445 and 485 nm) for quantification of chloramphenicol. An enzyme is not needed. ThT is added to the system as a fluorescent DNA probe. All this strongly reduces the cost for sensor construction and usage. The dual signal amplification steps occur simultaneously which reduces the detection time. The assay was successfully employed to the determination of CAP in spiked milk and fish samples within 60 min and with a 16 pM limit of detection (at S/N = 3).

    关键词： Food safety,Antibiotics detection,Thioflavin T,Catalyzed hairpin assembly,Target recycling

    更新于2025-11-19 16:46:39

  • Water-sensitive ratiometric fluorescent probes and application to test strip for rapid and reversible detection of water

    摘要： Naphthalimide-decorated fluorinated acetamides 1 and 2 were developed as solvent-sensitive dual emissive fluorescence probes. Particularly, 1 exhibited dual emission with a large Stokes shift for water, DMF, and DMSO solvents over other various organic solvents. The dual emission might be due to the increase of intramolecular charge transfer (ICT) of the naphthalimide moiety through the association of the difluorinated acetamide group with the solvent molecules. The 1 can give rise to a ratiometric change in the dual emission and a visual fluorescent color change depending on the water contents in organic solvents, including ethanol, methanol, acetonitrile and DMF solvents. Moreover, the 1-impregnated paper strips showed a rapid and easy-to-visualize fluorescent color change enabling water detection in organic solvents. These simple-to-use paper strips were also found to be reusable over 20 times.

    关键词： Naphthalimide,Ratiometric fluorescence probe,Reusable test strip,Intramolecular charge transfer (ICT),Water detection

    更新于2025-11-19 16:46:39

  • Highly selective detection of Fe3+, Cd2+ and CH2Cl2 based on a fluorescent Zn-MOF with azine-decorated pores

    摘要： The design and synthesis of sensitive and selective luminescent materials as chemical sensing agents is a fundamental goal in fluorescence assays. Considering high porosity, large surface area, excellent photoluminescence property of metal-organic frameworks (MOFs), luminescent properties of a microporous azine-functionalized MOF, TMU-16, dispersed in different metal ions and solvents have been investigated systematically. The TMU-16 displays superb luminescence emission, and it can detect Fe(III) and Cd(II) ions with high selectivity, excellent sensitivity, and short response time (<1 min). The emission intensities of TMU-16 were quenched upon the addition of Fe3+ and increased upon the addition of Cd2+. The detection limits of TMU-16 for Fe3+/Cd2+ in DMF are estimated to be 0.2 and 0.5 μM, respectively. The effect of other metal ions on the fluorescence intensity of the MOF was also studied and other metal ions showed low interference response in recognition of Fe3+ and Cd2+. Furthermore, TMU-16 exhibits distinct solvent-dependent luminescent spectra with emission intensity significantly enhanced toward dichloromethane. More importantly, this is the first example of MOF-based luminescent sensor as efficient multifunctional fluorescence material which can use for selective sensing of Fe(III) and Cd(II) ions and small molecules such as CH2Cl2.

    关键词： Small molecules,Metal ions,Luminescent,Sensing,Metal-organic frameworks,Detection

    更新于2025-11-19 16:46:39

  • Latent fingermark detection using functionalised silicon oxide nanoparticles: Method optimisation and evaluation

    摘要： The application of nanoparticles for latent fingermark detection has been reported in the literature over the past two decades. One of the nanoparticles that shows promise to become a routine technique is functionalised silicon oxide nanoparticles (SiO2 NPs). In a recent optimisation of the technique, the use of carboxyl-functionalised SiO2 NPs doped with luminescent ruthenium complex was proposed as a breakthrough for latent fingermark detection. In this study, the aforementioned functionalised SiO2 NPs were extensively evaluated. Modification and optimisation of the original detection parameters were performed to enhance detection quality and improve applicability. Various detection parameters were evaluated and assessed. A lower concentration of the functionalised nanoparticles used in the colloidal dispersion was determined to offer improved detection effectiveness. A combination of increased bath temperature and reduced immersion time was found to produce good overall results. A set of modified detection parameters was suggested for the use of the functionalised SiO2 NPs to detect latent fingermarks. Performance of the modified detection parameters was compared against that of the published detection method. Comparison experiments were carried out on fingermark specimens deposited on aluminium foil, transparent polypropylene plastic and green polyethylene plastic. Three donors (weak, average and strong) and two age intervals (ten days and three months) were considered in the comparison study. Evaluation of the results suggested that the overall performance of the modified method for latent fingermark detection was superior to that obtained using the previously published detection parameters.

    关键词： Bath temperature,Detection effectiveness,Method modification,Colloidal,Surface functionalisation,Luminescence technique

    更新于2025-11-19 16:46:39

  • A gadolinium(III)-porphyrin based coordination polymer for colorimetric and fluorometric dual mode determination of ferric ions

    摘要： A coordination polymer (CP) based nanoprobe is described for colorimetric and fluorometric (dual mode) determination of ferric ion. The method is making use of a nanosized Gd(III)?5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin coordination polymer that was prepared by a single-step hydrothermal procedure. The nanoprobe is monodisperse and has uniform size and good water solubility. It also exhibits strong fluorescence and magnetic resonance response. On exposure to Fe(III), the color of the solution changes from red to brown as the concentration of Fe(III) exceed 5 μM. Similarly, the red fluorescence of the probe (with excitation/emission peaks at 420/675 nm) decreases as concentrations of Fe(III) increase from 0.5 to 100 μM. The limit of detection is 98 nM in the fluorometric mode. The assay was applied to the determination of Fe(III) in fetal bovine serum samples.

    关键词： Fluorometry,Metal-organic compound,Fe3+ detection,Colorimetry,Gd-coordinated

    更新于2025-11-14 17:04:02

  • Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus

    摘要： Nanomaterial-based artificial enzymes or nanozymes exhibit superior properties such as stability, cost effectiveness and ease of preparation in comparison to conventional enzymes. However, the lower catalytic activity of nanozymes limits their sensitivity and thereby practical applications in the bioanalytical field. To overcome this drawback, herein we propose a very simple but highly sensitive, specific and low-cost dual enhanced colorimetric immunoassay for avian influenza A (H5N1) virus. 3,3′,5,5′- Tetramethylbenzidine (TMBZ) was used as a reducing agent to produce gold nanoparticles (Au NPs) with blue colored solution from a viral target-specific antibody-gold ion mixture at first step. The developed blue color from the sensing design was further amplified through catalytic activity of Au NPs in presence of TMBZ–hydrogen peroxide (H2O2) solution in second step. Hence, the developed dual enhanced colorimetric immunosensor enables the detection of avian influenza virus A (H5N1) with a limit of detection (LOD) of 1.11 pg/mL. Our results confirmed that the developed assay has superior sensitivity than the conventional ELISA method, plasmonic-based bioassay and commercial flu diagnostic kits. Proposed sensing method further showed its capability to detect viruses, avian influenza A (H4N6) and A (H9N2) virus, in blood samples with limit of detection of 0.0269 HAU and 0.0331 HAU respectively.

    关键词： Peroxidase mimic,Dual color enhancement,Gold nanoparticles,Avian influenza virus detection

    更新于2025-11-14 17:04:02

  • Photoelectrochemical biosensor for microRNA detection based on a MoS2/g-C3N4/black TiO2 heterojunction with Histostar@AuNPs for signal amplification

    摘要： Herein, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of microRNA-396a based on a MoS2/g-C3N4/black TiO2 heterojunction as the photoactive material and gold nanoparticles carrying Histostar antibodies (Histostar@AuNPs) for signal amplification. Briefly, MoS2/g-C3N4/black TiO2 was deposited on an indium tin oxide (ITO) electrode surface, after which gold nanoparticles (AuNPs) and probe DNA were assembled on the modified electrode. Hybridization with miRNA-396a resulted in a rigid DNA:RNA hybrid being formed, which was recognized by the S9.6 antibody. The captured antibody can further conjugate with the secondary IgG antibodies of Histostar@AuNPs, thereby leading to the immobilization of horse radish peroxidase (HRP). In the presence of HRP, the oxidation of 4-chloro-1-naphthol (4-CN) by H2O2 was accelerated, producing the insoluble product benzo-4-chlorohexadienone on the electrode surface and causing a significant decrease in the photocurrent. The developed biosensor could detect miRNA-396a at concentrations from 0.5 fM to 5000 fM, with a detection limit of 0.13 fM. Further, the proposed method can also be used to investigate the effect of heavy metal ions on the expression level of miRNAs. Results suggest that the biosensor developed herein offers a promising platform for the ultrasensitive detection of miRNA.

    关键词： S9.6 antibody,Histostar@AuNPs,MicroRNA detection,MoS2/g-C3N4/black TiO2 heterojunction,Photoelectrochemical biosensor

    更新于2025-11-14 17:04:02

  • Dy(III)-induced aggregation emission quenching effect of single-layered graphene quantum dots for selective detection of phosphate in the artificial wetlands

    摘要： Carbon quantum dots (CQDs), prepared by one-step hydrothermal treatment of perylene-3,4,9,10-tetra-carboxylic dianhydride (PTCDA) and triethylamine (TEA), could be exfoliated or delaminated into single-layered graphene quantum dots (s-GQDs) with methanol for the first time, with fluorescence (FL) emission at 500 nm when excited at 417 nm. The s-GQDs, with more sufficient carboxyl groups on the surface than CQDs, could be induced to be aggregated by metal ion dysprosium (Dy3+), resulting in aggregation-induced emission quenching effect subsequently. However, the presence of phosphate (PO4 3-) destroys the Dy3+-induced aggregates of s-GQDs owing to the strong coordination between Dy3+ and PO4 3-, inducing the FL emission recovery of the s-GQDs and providing selective detection method of PO4 3- in the artificial wetlands with the linear range of 0.2–30 μM and determination limit of 0.1 μM (3σ).

    关键词： s-GQDs-Dy3+ system,Phosphate detection,Single-layered graphene quantum dots,Carbon quantum dots

    更新于2025-11-14 17:04:02

  • Plasmonic Colorimetric Biosensor for Visual Detection of Telomerase Activity Based on Horseradish Peroxidase-Encapsulated Liposomes and Etching of Au Nanobipyramids

    摘要： Telomerase aberrant activation is a critical feature in the vast majority of cancers. To visualize telomerase expression level in tumor cells, we developed a plasmonic colorimetric sensor for highly sensitive detection of telomerase activity by integrating an excellent etching substrate Au nanobipyramids (Au NBPs) with a liposome-based signal amplification strategy. Upon telomerase-triggered extension, the telomerase activity was converted into the amount of the attached horseradish peroxidase-encapsulated liposomes (HRP-Ls) on the surfaces of magnetic beads. Afterwards, HRP was liberated from the liposomes following the addition of H2O2-3,3′,5,5′-tetramethylbenzidine sulfate (TMB) substrate, and then the oxidation reaction between H2O2 and TMB was initiated to form TMB2+. The morphological evolution of Au NBPs relied on the TMB2+-mediated etching reaction, which gave rise to tremendous localized surface plasmon resonance (LSPR) responses and concomitant tonality transitions. Benefiting from cascaded signal amplification capacity of HRP-Ls and the intriguing optical properties of Au NBPs, an impressive sensitivity toward telomerase was obtained with detection limits equivalent to 1 HeLa cell for LSPR peak measurement and a visual detection limit of 20 HeLa cells. Furthermore, a facile and portable kit was fabricated for visual evaluation of telomerase activity in different cell lines.

    关键词： Plasmonic colorimetry,Au nanobipyramids,Telomerase,Liposome-based amplification,Multicolor visual detection

    更新于2025-11-14 17:04:02