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Luminescent Lanthanide–Collagen Peptide Framework for pH-Controlled Drug Delivery
摘要： Collagen mimetic scaffolds play a pivotal role in regenerative medicine and tissue engineering due to their extraordinary structural and biological features. We have herein for the first time reported the construction of luminescent lanthanide-collagen peptide hybrid three-dimensional nanofibrous scaffolds, which well mimic the characteristic architectural structure of native collagen. Three collagen mimetic peptides composed of repetitive central (GPO)7 sequences and altered terminal amino acids, have been shown to consistently self-assemble to form biocompatible nanofibers under the trigger of a variety of lanthanide ions, which also functionalize the assembled materials with easily tunable photoluminescence. Furthermore, the collagen peptide-lanthanide hybrid scaffolds possess programmable pH-responsive features. The lanthanide ion-mediated assembly of all the three collagen peptides are conveniently and reversibly regulated by pH, while their pH-dependent patterns are finely tuned by the identity of terminal amino acids. Using camptothecin and cefoperazone sodium as two model drugs, the drug-loading and releasing efficiency of the collagen peptide-lanthanide scaffolds are nicely modulated by pH, demonstrating the efficacy of these nanofibrous scaffolds as pH-responsive drug carriers. These novel luminescent collagen peptide-lanthanide scaffolds provide a facile system for pH-controlled drug delivery, suggesting promising applications in the development of therapies for many diseases.
关键词： self-assembly,scaffold,pH-responsive,Collagen mimetic peptides,drug delivery
更新于2025-09-23 15:23:52
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Pd nanoparticle-decorated hydroxy boron nitride nanosheets as a novel drug carrier for chemo-photothermal therapy
摘要： A multi-stimuli responsive nanoagent, hydroxy boron nitride nanosheets and Pd nanohybrids (Pd@OH-BNNS), was studied. The well-dispersed hydroxy boron nitride nanosheets were prepared via a facile thermal substitution approach. Pd@OH-BNNS was endowed with a photothermal property after in situ formation of Pd nanoparticles on the surface of the nanosheets. Pd@OH-BNNS as a drug delivery carrier exhibited high loading capability for the anticancer drug doxorubicin. The release of doxorubicin from the nanohybrids was triggered by a decrease in pH, and increases in glutathione concentration and near-infrared irradiation. The faster release behavior of the nanohybrids under near-infrared irradiation was confirmed by a cellular uptake study. In addition, local hyperthermia was verified using an imaging test and an infrared thermal camera. Tumor growth was remarkably inhibited in mice after two weeks of treatment with Pd@OH-BNNS/doxorubicin injection, demonstrating the high efficacy of combining chemotherapy and photothermal therapy. The Pd@OH-BNNS as a drug delivery system exhibited good stability, low cytotoxicity and multi-stimuli responsiveness.
关键词： photothermal therapy,Pd nanoparticles,drug delivery system,hydroxy boron nitride,nanohybrids
更新于2025-09-23 15:23:52
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Toward a new age of patient centricity? The application of eye-tracking to the development of connected self-injection systems
摘要： Background: Increasing interest in digitally enhanced drug delivery tools urges industry and academia to rethink current approaches to product usability. This article introduces mobile eye-tracking, generating detailed contextual data about user engagement with connected self-injection systems as a new methodological approach to formative usability assessment. Methods: A longitudinal case study with a total of 35 injection-na?ve participants was conducted. In three consecutive experiments, eye-tracking was applied to formative usability testing of a novel connected self-injection device. Three eye-tracking derived usability indicators were established to assess product effectiveness, efficiency, and ease of use. Results: Analysis of the data revealed events of user hesitation, process interruption and unintended action, and these occurrences could either be completely eliminated or significantly reduced throughout the process. At the same time, the overall use duration decreased from 86.1 to 58.7 sec. Analysis revealed that product modifications successfully guided user attention to those interface elements most relevant during each task, thereby improving product ease-of-use. Conclusions: The step-wise improvement in the usability indicators demonstrates that iteratively applying eye-tracking methods effectively supports the user-centered design of connected self-injection systems. The results highlight how eye-tracking can be employed to gain an in-depth understanding of patient engagement with novel healthcare technologies.
关键词： smart devices,digital health,eye-tracking,self-injection devices,Connected drug delivery,patient centricity,formative usability
更新于2025-09-23 15:23:52
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Dual-responsive molybdenum disulfide/copper sulfide-based delivery systems for enhanced chemo-photothermal therapy
摘要： Molybdenum disul?de (MoS2)-based drug delivery systems have shown considerable potential in cancer nanomedicines. In this work, a multifunctional nanoplatform comprising MoS2 nanosheets decorated with copper sul?de (CuS) and further functionalized with polyethylene glycol (PEG) is reported. The resultant material has a particle size of approximately 115 nm, and can be loaded with doxorubicin (DOX) to a loading capacity of 162.3 mg DOX per g of carrier. Drug release is triggered by two stimuli (near infrared (NIR) irradiation and pH), and the carrier is shown to have excellent colloidal stability. The presence of both MoS2 and CuS leads to very high photothermal conversion ef?ciency (higher than with MoS2 alone). In vitro experiments revealed that the blank CuS-MoS2-SH-PEG carrier is biocompatible, but that the synergistic application of chemo-photothermal therapy (in the form of CuS-MoS2-SH-PEG loaded with DOX and NIR irradiation) led to greater cell death than either chemotherapy (CuS-MoS2-SH-PEG(DOX) but no NIR) or photothermal therapy (CuS-MoS2-SH-PEG with NIR). A cellular uptake study demonstrated that the nanoplatform can ef?ciently enter tumor cells, and that uptake is enhanced when NIR is applied. Overall, the functionalized MoS2 material developed in this work exhibits great potential as an ef?cient system for dual responsive drug delivery and synergistic chemo-photothermal therapy. The route employed in our work thus provides a strategy to enhance photothermal ef?cacy for transition metal dichalcogenide drug delivery systems.
关键词： Chemotherapy,Drug delivery,MoS2,Photothermal therapy,Synergistic therapy
更新于2025-09-23 15:23:52
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An inorganic magnetic fluorescent nanoprobe with favorable biocompatibility for dual-modality bioimaging and drug delivery
摘要： In this work, we present the development of a high water soluble biological nanoprobe through the covalent bonding of β-CD onto the surface of iron oxide?gold nanoclusters (Fe3O4@Au@β-CD). Fe3O4@Au@SiO2 NPs were also prepared for comparison. The maximum emission peak of magnetic fluorescent NPs red-shifted by 30 nm and the lifetime was also elongated to 5.21 μs after surface modified with β-CD. The relaxivities and in vitro magnetic resonance imaging ability of the resultant magnetic fluorescent NPs were also studied, indicating that Fe3O4@Au@β-CD NPs have the lowest r2/r1 ratio and could be a potential T2 contrast agent for MR imaging. The MTT assay proved that, Fe3O4@Au@β-CD NPs are of excellent water solubility and biocompatibility. In vitro confocal fluorescence imaging was also performed, manifesting that Fe3O4@Au@β-CD NPs can be selectively uptaken by gastric cancer cells (MGC-803) and exhibit red fluorescence in the cells. The preliminary drug loading and releasing measurements demonstrate that it can also act as targeted drug delivery nanosystem. All these experimental results indicate that, Fe3O4@Au@β-CD NPs hold great application perspective in the diagnosis and therapy of gastric cancer cells as biological nanoprobes.
关键词： Multifunctional,Biocompatibility,Magnetic fluorescent nanocomposites,Drug delivery,Dual-modality imaging
更新于2025-09-23 15:23:52
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Pharmacokinetic Simulations of Intravitreal Biologicals: Aspects of Drug Delivery to the Posterior and Anterior Segments
摘要： Biologicals are important ocular drugs that are be delivered using monthly and bimonthly intravitreal injections to treat retinal diseases, such as age-related macular degeneration. Long acting delivery systems are needed for prolongation of their dosing interval. Intravitreal biologicals are eliminated from the eye via the aqueous humor outflow. Thus, the anterior and posterior segments are exposed to the drug. We utilized a kinetic simulation model to estimate protein drug concentrations in the vitreous and aqueous humor after bolus injection and controlled release administration to the vitreous. The simulations predicted accurately the experimental levels of 5 biologicals in the vitreous and aqueous humor. The good match between the simulations and experimental data demonstrated almost complete anterior segment bioavailability, and major dose sparing with ocular controlled release systems. Overall, the model is a useful tool in the design of intraocular delivery of biologicals.
关键词： aflibercept,ocular drug delivery,ranibizumab,intravitreal injection,controlled release,bevacizumab
更新于2025-09-23 15:22:29
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Polypyrrole microcapsules loaded with gold nanoparticles: Perspectives for biomedical imaging
摘要： We report the facile preparation of polypyrrole microcapsules with a hydrophobic liquid core that is loaded with gold nanoparticles. Through the oxidative polymerization of pyrrole, the polymer is deposited onto the surface of the droplets, which results in the encapsulation of both the liquid phase and the metal nanoparticles. We demonstrate the preparation of the microcapsules loaded with organic solvents (toluene, hexane) or 2-oxoheptyl isothiocyanate (new promising anticancer agent) as the liquid cores and stable or radioactive gold nanoparticles (Au-197 or Au-198 isotopes). The resulting microcapsules have been demonstrated as promising agents for medical applications such as computed tomography or gamma imaging. Moreover, the capsules can be applied as drug carriers, which has been shown in vitro on cancer and normal cell cultures.
关键词： Targeted drug delivery,Gold nanoparticles doped with Au-198,Computed tomography,Anticancer agents,Polypyrrole microcapsules,Gamma imaging
更新于2025-09-23 15:22:29
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Biodegradable Micelles for NIR/GSH-Triggered Chemophototherapy of Cancer
摘要： The chemotherapy of stimuli-responsive drug delivery systems (SDDSs) is a promising method to enhance cancer treatment effects. However, the low efficiency of chemotherapy drugs and poor degradation partly limit the application of SDDSs. Herein, we report doxorubicin (DOX)-loading mixed micelles for biotin-targeting drug delivery and enhanced photothermal/photodynamic therapy (PTT/PDT). Glutathione (GSH)-responsive mixed micelles were prepared by a dialysis method, proportionally mixing polycaprolactone-disulfide bond-biodegradable photoluminescent polymer (PCL-SS-BPLP) and biotin-polyethylene glycol-cypate (biotin-PEG-cypate). Chemically linking cypate into the mixed micelles greatly improved cypate solubility and PTT/PDT effect. The micelles also exhibited good monodispersity and stability in cell medium (~119.7 nm), low critical micelles concentration, good biodegradation, and photodecomposition. The high concentration of GSH in cancer cells and near-infrared light (NIR)-mediated cypate decomposition were able to achieve DOX centralized release. Meanwhile, the DOX-based chemotherapy combined with cypate-based NIR-triggered hyperthermia and reactive oxygen species could synergistically induce HepG2 cell death and apoptosis. The in vivo experiments confirmed that the micelles generated hyperthermia and achieved a desirable therapeutic effect. Therefore, the designed biodegradable micelles are promising safe nanovehicles for antitumor drug delivery and chemo/PTT/PDT combination therapy.
关键词： photothermal therapy,photodynamic therapy,biodegradable,stimuli-responsive drug delivery systems,cypate
更新于2025-09-23 15:22:29
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Stimuli-Responsive Nanotheranostics for Real-Time Monitoring Drug Release by Photoacoustic Imaging
摘要： Molecular photoacoustic imaging (PA) is a promising technology to understand tumor pathology and guide precision therapeutics. Despite the capability of activatable PA probes to image tumor-specific biomarkers, limitations in their molecular structure hamper them from effective drug delivery and the drug release monitoring. Herein, we developed a perylene diimide (PDI) based theranostic platform that provides noninvasive PA imaging signals to monitor tumor-specific pH-responsive drug release. Methods: we first designed and synthesized an acid-responsive amine-substituted PDI derivative. The pH sensitive properties of the PDI was demonstrated by density functional theory (DFT) calculations, UV-vis experiments and PA studies. The theranostic platform (THPDINs) was fabricated by self-assembly of the acid-responsive PDI, a pH irrelevant IR825 dye, and anti-cancer drug doxorubicin (DOX). The PA properties in various pH environment, drug delivery, cytotoxicity, cell uptake, ratiometric PA imaging and anti-tumor efficacy of the THPDINs were investigated in vitro and in vivo by using U87MG glioma cell line and U87MG tumor model. Results: We found that our designed PDI was sensitive to the tumor specific pH environment, reflected by absorbance shift, PA intensity and aggregation morphology changes in aqueous solution. The as-synthesized pH sensitive PDI acted as a molecular switch in the THPDINs, in which the switch can be triggered in the mild acidic tumor microenvironment to accelerate DOX release. Meanwhile, the DOX release could be monitored by ratiometric PA imaging. Conclusions: We developed a multifunctional PDI based theranostic platform for noninvasive real-time ratiometric PA imaging of tumor acidic pH and monitoring of drug release in living mice simultaneously. This strategy will shed light on the development of smart activatable theranostic nanoplatforms and will significantly advance the application of PA theranostics in biology and medicine.
关键词： photoacoustic imaging,drug delivery,pH-responsive,ratiometric imaging,theranostics
更新于2025-09-23 15:22:29
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Layer-by-layer Modification of Magnetic Graphene Oxide by Chitosan and Sodium Alginate with Enhanced Dispersibility for Targeted Drug Delivery and Photothermal Therapy
摘要： In this work, graphene oxide nanosheets loaded by magnetic iron oxide nanoparticles (mGO) was synthesized and the technique of layer-by-layer (LbL) self-assembly was utilized in the successful production of chitosan/ sodium alginate functionalized mGO naocomposites for use in targeted anti-cancer drug delivery and photothermal therapy. The mGO-CS/SA nanocomposites had a diameter of ~0.5 μm and a thickness of 40-60 nm with superparamagnetic behavior. The modified nanocomposites exhibited a decrease in agglomeration and an increase in stability in biological solution following stability tests. Meanwhile, the nonspecific protein adsorption was strongly suppressed after the modification. The mGO-CS/SA nanocomposites were loaded with doxorubicin hydrochloride (DOX) via π- π stacking and electrostatic attraction with a high drug loading amount (121%, w/w). The DOX-loaded nanocomposites (mGO-CS/SA-DOX) showed improvements in function including enhanced dispersion and noticeable pH-sensitive drug release behavior. Cellular studies denoted magnetically targeted cellular uptake characteristics and excellent photothermal effect of mGO-CS/SA, as well as concentration-dependent cytotoxicity of mGO-CS/SA-DOX. Therefore the functionalization of mGO using chitosan and sodium alginate would be beneficial in biomedical applications.
关键词： magnetic nanocomposite,Layer-by-layer,Graphene oxide,photothermal therapy,drug delivery
更新于2025-09-23 15:22:29