- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Development of a longevous two-species biophotovoltaics with constrained electron flow
摘要： Microbial biophotovoltaics (BPV) offers a biological solution for renewable energy production by using photosynthetic microorganisms as light absorbers. Although abiotic engineering approaches, e.g., electrode modification and device optimization, can enhance the electrochemical communication between living cells and electrodes, the power densities of BPV are still low due to the weak exoelectrogenic activity of photosynthetic microorganisms. Here, we develop a BPV based on a D-lactate mediated microbial consortium consisting of photosynthetic cyanobacteria and exoelectrogenic Shewanella. By directing solar energy from photons to D-lactate, then to electricity, this BPV generates a power density of over 150 mW·m?2 in a temporal separation setup. Furthermore, a spatial-temporal separation setup with medium replenishment enables stable operation for over 40 days with an average power density of 135 mW·m?2. These results demonstrate the electron flow constrained microbial consortium can facilitate electron export from photosynthetic cells and achieve an efficient and durable power output.
关键词： biophotovoltaics,electron flow,microbial consortium,cyanobacteria,renewable energy,D-lactate,Shewanella
更新于2025-09-12 10:27:22
-
Drought-induced changes in photosynthetic electron transport in maize probed by prompt fluorescence, delayed fluorescence, P700 and cyclic electron flow signals
摘要： The effect of drought on the prompt chlorophyll a fluorescence (PF) transient (OJIP), delayed chlorophyll a fluorescence (DF), modulated 820-nm reflection (MR), energy conversion efficiencies in photosystems (PS) I and II, and cyclic electron flow (CEF) activity in two maize hybrids with contrasting drought tolerance was investigated. Our aim was to identify the target site of drought stress on the photosynthetic electron transport chain and investigate the relevance of the CEF pathway to the drought tolerance of maize plants. The OJIP analysis showed that drought stress, depending on its duration, decreased FP, increased FJ, and induced a pronounced K-band and a positive L-band. Moreover, OJIP parameters, including PIABS, RC/CSO, TRO/ABS, and ETO/TRO, were significantly reduced. The DF analysis showed that the values of I1 and I2 in the induction curve and L1 and L2 derived from the decay curve decreased progressively with the duration of drought stress. The MR analysis showed that drought stress inactivated both the fast decrease and slow increase phases of the MR transient, resulting in a gradual decrease in both VPSI and VPSII-PSI. The energy conversion analysis showed that drought stress decreased the PSI photochemical quantum yield Y(I) and PSII photochemical quantum yield Y(II). Compared to the tolerant hybrid, the drought-induced changes in the sensitive hybrid were stronger and appeared at an earlier treatment stage. The CEF activity analysis showed that the CEF pathway under drought stress operated for a longer time in the tolerant hybrid than that in the sensitive hybrid. The above results indicate that drought stress damaged the donor and acceptor sides of PSII, the PSII reaction center and the acceptor side of PSI and decreased the efficiency of both PSI and PSII and the capacity of electron transfer. The CEF pathway might play an important role in the tolerance of the maize photosynthetic electron transport chain to drought stress.
关键词： Cyclic electron flow,Delayed fluorescence,Modulated 820 nm reflection,Energy conversion efficiencies in photosystems (PS) I and II,Prompt fluorescence OJIP,Maize,Drought stress
更新于2025-09-10 09:29:36