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Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry
摘要: Non-additive enhancement of the photosynthesis excited by simultaneous illumination with far-red light and light of shorter wavelengths is called as “second Emerson effect”. Its action spectra are well-known as a photosynthetic yield’s dependence on light wavelength in red (630-690 nm) spectral region at a constant-wavelength far-red illumination near 700-715 nm. However, the opposite dependence of the photosynthetic yield’s of shorter constant-wavelength light (red or blue) on light wavelength in far-red (690-760 nm) spectral region was never studied. In this study the action spectrum of second Emerson effect was studied using a fast-Fourier dual-wavelength Pulse Amplitude Modulation (PAM) fluorometry. Chlorophyll fluorescence in ailanthus (Ailanthus altissima Mill.) leaves was excited with blue modulated light. Far-red induced decrease of fluorescence (fluorescence shift-FRIFS) was studied in response to illumination of leaves with a background light from 690 to 760 nm (10 nm step), calculating FRIFS = (F0-Fs)/F0, where F0-fluorescence measured without and Fs-with far-red light. Maximum FRIFS was observed at 720 nm (11.8%), but it still remained considerable at 740, 750 nm and a low FRIFS values were revealed at 690 and even at 760 nm. Measurements carried out with blue saturating flashes during and after far-red illumination showed the increase of quantum yield of Photosystem II (PSII), calculated as Fv/Fm at 720 nm background light. FRIFS had lower values under excitation with red modulating light. It is concluded that FRIFS is a result of a photochemical quenching caused by an additional selective far-red excitation of PSI in conditions when PSII is preferably excited by blue light thus leading the PSI to limit non-cyclic electron flow. The contradiction between the known absorption spectra of PSI-light harvesting complex I and the observed action spectrum of second Emerson effect (FRIFS spectrum) is discussed.
关键词: Photosystem II,Ailanthus Altissima,Photosystem I,Second Emerson Effect,Fast-Fourier PAM-Fluorometry,Far-Red Light,Thylakoid Electron Transport
更新于2025-11-14 15:30:11
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A review on smart application of supplemental lighting in greenhouse fruiting vegetable production
摘要: Different spectra of light trigger different plant growth processes. Therefore, the optimum light spectrum for various plant physiological and growth processes may be different. For greenhouse fruiting vegetables, such as tomatoes, it is important to optimize light spectrum to promote canopy growth to increase light interception during the early stage of plant growth. Once the plants reach full canopy, the focus should be moved to optimizing vertical light distribution because most greenhouse fruiting vegetables are tall crops and the growth processes along the vertical profile are different; most of the canopy growth occurs in the top and middle canopy while fruit growth occurs in the middle and bottom canopy. Because the fruit, not the leaf, is the economic product, the optimized light spectral composition should enhance leaf carbon export and translocation to fruit to improve fruit yield and quality. Therefore, a research project was initiated in 2013 to identify proper light spectral composition and vertical distribution regimes for greenhouse fruiting vegetable production. Different overhead light sources (high-pressure sodium light with or without far-red light-emitting diode (LED) light, plasma light and different spectral compositions of LEDs) and several intra-canopy spectral compositions provided by LEDs were evaluated over four winters on tomatoes, mini-cucumbers and sweet peppers. The effects of light spectrum on whole-plant net carbon exchange and leaf carbon export using 14C-isotype tracing were also investigated. The vertical light regimes resulted in significant differences in leaf photosynthetic rate, leaf size, fruit yield and fruit quality in greenhouse tomatoes, mini-cucumbers and sweet peppers. Proper vertical light regimes were identified for hybrid light systems (overhead high intensity discharge light + intra-canopy LEDs) and for pure LED light systems (overhead + intra-canopy LEDs). This review clearly demonstrates that optimized vertical light regimes can be developed for improving both plant growth and fruit yield and quality in year-round greenhouse fruiting vegetable production.
关键词: far-red light,Solanum lycopersicum,Capsicum annuum,Cucumis sativus,light quality,vertical light spectral distribution,temperature,LED,lighting,spectral composition
更新于2025-09-23 15:22:29
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Overhead supplemental far-red light stimulates tomato growth under intra-canopy lighting with LEDs
摘要: Far-red (FR) light regulates phytochrome-mediated morphological and physiological plant responses. This study aims to investigate how greenhouse tomato morphology and production response to different durations of FR light during daytime and at the end of day (EOD). High-wire tomato plants were grown under intra-canopy lighting consisting of red (peak wavelength at 640 nm) and blue (peak wavelength at 450 nm) light-emitting diodes (LEDs) with photosynthetic photon flux density (PPFD) of 144 μmol m–2 s–1 at 10 cm away from the lamps, and combined with overhead supplemental FR light (peak wavelength at 735 nm) with PPFD of 43 μmol m–2 s–1 at 20 cm below the lamps. Plants were exposed to three durations of FR supplemental lighting including: 06:00–18:00 (FR12), 18:00–19:30 (EOD-FR1.5), 18:00–18:30 (EOD-FR0.5), and control that without supplemental FR light. The results showed that supplemental FR light significantly stimulated stem elongation thereby resulting in longer plants compared with the control. Moreover, FR light altered leaf morphology toward higher leaf length/width ratio and larger leaf area. The altered plant architecture in FR supplemented plants led to a more homogeneous light distribution inside the canopy. Total plant biomass was increased by 9–16% under supplemental FR light in comparison with control, which led to 7–12% increase in ripe fruit yield. Soluble sugar content of the ripe tomato fruit was slightly decreased by longer exposure of the plants to FR light. Dry matter partitioning to different plant organs were not substantially affected by the FR light treatments. No significant differences were observed among the three FR light treatments in plant morphology as well as yield and biomass production. We conclude that under intra-canopy lighting, overhead supplemental FR light stimulates tomato growth and production. And supplementary of EOD-FR0.5 is more favorable, as it consumes less electricity but induces similar effects on plant morphology and yield.
关键词: intro-canopy lighting,morphology,far-red light,LEDs,yield,Solanum lycopersicum
更新于2025-09-12 10:27:22