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Chromatographic Fingerprint Analysis of Radix Hedysari Using Supercritical Fluid Chromatography Coupled with Diode Array Detector
摘要： A newly and rapid supercritical ?uid chromatography method for the simultaneous determination of 11 active compounds in Radix Hedysari samples has been developed and validated. Optimum separation was achieved on a HSS SB C18 column with a gradient elution at a ?ow rate of 1.5 mL/min, back pressure of 11.03 Mpa and diode array detector at 260 nm. The results from the quantitative data showed that contents of these 11 active compounds were different from plant regions. Especially the contents of formononetin in the Minxian county are ～6-fold than in wild Radix Hedysari. The chromatographic ?ngerprint of Radix Hedysari was recorded under the same chromatographic condition. Data analytic procedure was performed to differentiate the 25 batches of Radix Hedysari samples. Data from chromatographic ?ngerprint were also analyzed using hierarchical cluster analysis. The results showed that 23 batches of Radix Hedysari samples had a high similarity (> 0.90) and overall 25 batches of sample were divided into two clusters. Moreover, according to the comparison contents of active compounds in each Radix Hedysari samples, the cultivated location of Radix Hedysari was successfully distinguished. This method presented good stability, repeatability and precision and would be a useful and reliable approach for the quality control of Radix Hedysari. Moreover, all target compounds were quanti?ed by ultra-high performance liquid chromatography–time-of-?ight mass spectrometry.
关键词： Radix Hedysari,supercritical ?uid chromatography,hierarchical cluster analysis,chromatographic ?ngerprint,quality control
更新于2025-09-19 17:13:59
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Quantitative Analysis of Twelve Active Components Combined With Chromatographic Fingerprint for Comprehensive Evaluation of Qinma Prescription by Ultra-Performance Liquid Chromatography Coupled With Diode Array Detection
摘要： A combination method of ultra-performance liquid chromatography (UPLC) coupled with diode array detection has been developed for quality evaluation of Qinma prescription (QMP), based on chromatographic fingerprint technology with the similarity analysis (SA) and the quantitative analysis of 12 components by hierarchical cluster analysis (HCA). The established method has been validated by linearity, precision, repeatability, stability and recovery tests. The UPLC fingerprints with 17 common peaks of 5 QMP samples prepared by different extraction methods including water decoction extraction, water extraction-ethanol precipitation method, ethanol reflux extraction, ethanol extraction-water precipitation method and methanol ultrasonic extraction were obtained, and the SA results indicated that similarity index was greatly influenced by the large peak. The similarity index ranged from 0.816 to 0.999 basing on 17 peaks, which has been decreased to 0.683–0.999 basing on 16 peaks without the large peak of baicalin (BA). The results of simultaneous quantification of 12 components in these 5 QMP samples proved that BA, gallic acid (GA), wogonoside (WOG) and gentiopicroside (GEN) were the major ingredients in QMP with high contents >1.44 (mg/g), indicating that ethanol reflux was the most effective extraction method. Integrating fingerprint analysis, simultaneous determination and HCA, the established method is rapid, sensitive, accurate and readily applicable. All the results indicated that the combination method can control the quality of QMP and its related traditional Chinese medicinal compounds more comprehensively and scientifically.
关键词： quantitative analysis,Qinma prescription,similarity analysis,diode array detection,UPLC,hierarchical cluster analysis,chromatographic fingerprint
更新于2025-09-12 10:27:22
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Rapid subtyping of pathogenic and nonpathogenic Vibrio parahaemolyticus by fourier transform infrared spectroscopy with chemometric analysis
摘要： Vibrio parahaemolyticus which naturally inhabits marine and estuarine environment represents pathogenic strains (virulence genes tdh or trh positive) and non-pathogenic strains (virulence genes negative). In this study, a rapid method for subtyping pathogenic and non-pathogenic V. parahaemolyticus was established using fourier transform infrared (FTIR) spectroscopy with chemometric analysis. This method targeted three strains of genotypes of V. parahaemolyticus including tdh positive, trh positive and virulence gene-negative (nonpathogenic) V. parahaemolyticus. The FTIR absorption spectra between 1800 and 900 cm?1 highlighted the most distinctive variations and were the most useful for characterizing the three bacteria. The successful differentiation and identification of the three bacteria could be accomplished in less than 1 h by FTIR using principal component analysis (PCA), or another cluster model of hierarchical cluster analysis (HCA). The method was verified by analyzing spiked V. parahaemolyticus fish samples. Furthermore, all of ten clinical isolates of V. parahaemolyticus were identified as tdh-positive, none of the clinical isolates were trh-positive, and all of ten environmental isolates were identified as non-pathogenic by the subtyping method, which were confirmed by PCR assays. All data demonstrated that the newly established subtyping method by FTIR is practical, time-saving, labor-saving, specific and cost-effective, especially suitable for the basic laboratories of CDC and port quarantine departments to perform suiveillance and epidemiological traceability of pathogenic V. parahaemolyticus.
关键词： Pathogenicity,Principal component analysis,Fourier transform infrared spectroscopy,Hierarchical cluster analysis,Vibrio parahaemolyticus,Subtype
更新于2025-09-09 09:28:46
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Discrimination of molecular thin films by surface-sensitive time-resolved optical spectroscopy
摘要： An optical discrimination technique, tailored to nanometric-sized, low optical absorbance molecular complexes adhering to thin metal films, is proposed and demonstrated. It is based on a time-resolved evanescent-wave detection scheme in conjunction with hierarchical cluster analysis and principal value decomposition. The present approach aims to differentiate among molecular films based on statistical methods, without using previous detailed knowledge of the physical mechanisms responsible for the detected signal. The technique is open to integration in lab-on-a-chip architectures and nanoscopy platforms for applications ranging from medical screening to material diagnostics.
关键词： time-resolved optical spectroscopy,principal value decomposition,optical discrimination technique,hierarchical cluster analysis,molecular thin films
更新于2025-09-09 09:28:46