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- 2017
- spectral reconstruction
- interference
- polarization
- transform
- imaging spectrometer
- Optoelectronic Information Science and Engineering
- BITTT
- Zhejiang University
- Guilin University of Aerospace Technology
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Quantitative Image Analysis of Cellular Morphology Using Amnis???? ImageStreamX Mark II Imaging Flow Cytometer: A Comparison against Conventional Methods
摘要： Chemotaxis, the directional cell migration guided by chemoattractant gradients, plays essential roles in many physiological processes, such as recruitment of neutrophils to sites of inflammation. Neutrophils detect chemoattractants by G protein-coupled receptors (GPCRs). Chemoattractant stimuli activate multiple signaling pathways to regulate directional migration of neutrophils. Recently, we identified a novel GPCR-mediated PLCβγ/ PKCβ/PKD1 signaling axis that regulates cofilin activity through cofilin phosphatase slingshot 2 (SSH2) and remodels actin cytoskeleton during neutrophil chemotaxis. In the future, it will be important to understand how multiple signaling pathways are spatiotemporally regulated to precisely control the rapid remodeling of actin cytoskeleton in the leading front of chemotaxing neutrophils.
关键词： Flow,Brightfield,Imaging,Fluorescence,Cytometry
更新于2025-09-23 15:23:52
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A Runtime-Scalable and Hardware-Accelerated Approach to On-Board Linear Unmixing of Hyperspectral Images
摘要： Space missions are facing disruptive innovation since the appearance of small, lightweight, and low-cost satellites (e.g., CubeSats). The use of commercial devices and their limitations in cost usually entail a decrease in available on-board computing power. To face this change, the on-board processing paradigm is advancing towards the clustering of satellites, and moving to distributed and collaborative schemes in order to maintain acceptable performance levels in complex applications such as hyperspectral image processing. In this scenario, hybrid hardware/software and reconfigurable computing have appeared as key enabling technologies, even though they increase complexity in both design and run time. In this paper, the ARTICo3 framework, which abstracts and eases the design and run-time management of hardware-accelerated systems, has been used to deploy a networked implementation of the Fast UNmixing (FUN) algorithm, which performs linear unmixing of hyperspectral images in a small cluster of reconfigurable computing devices that emulates a distributed on-board processing scenario. Algorithmic modifications have been proposed to enable data-level parallelism and foster scalability in two ways: on the one hand, in the number of accelerators per reconfigurable device; on the other hand, in the number of network nodes. Experimental results motivate the use of ARTICo3-enabled systems for on-board processing in applications traditionally addressed by high-performance on-Earth computation. Results also show that the proposed implementation may be better, for certain configurations, than an equivalent software-based solution in both performance and energy efficiency, achieving great scalability that is only limited by communication bandwidth.
关键词： FPGAs,hyperspectral imaging,on-board processing,ARTICo3,linear unmixing
更新于2025-09-23 15:23:52
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Mercury Free Microscopy: An Opportunity for Core Facility Directors
摘要： Mercury Free Microscopy (MFM) is a new movement that encourages microscope owners to choose modern mercury free light sources to replace more traditional mercury based arc lamps. Microscope performance is enhanced with new solid state technologies because they offer a more stable light intensity output and have a more uniform light output across the visible spectrum. Solid state sources not only eliminate mercury but also eliminate the cost of consumable bulbs (lifetime (cid:2)200 hours), use less energy, reduce the instrument down time when bulbs fail and reduce the staff time required to replace and align bulbs. With lifetimes on the order of tens of thousands of hours, solid state replacements can pay for themselves over their lifetime with the omission of consumable, staff (no need to replace and align bulbs) and energy costs. Solid state sources are also sustainable and comply with institutional and government body mandates to reduce energy consumption, carbon footprints and hazardous waste. MFM can be used as a mechanism to access institutional ?nancial resources for sustainable technology through a variety of stakeholders to defray the cost to microscope owners for the initial purchase of solid state sources or the replacement cost of mercury based sources. Core facility managers can take a lead in this area as “green” ambassadors for their institution by championing a local MFM program that will save their institution money and energy and eliminate mercury from the waste stream. Managers can leverage MFM to increase the visibility of their facility, their impact within the institution, and as a vital educational resource for scienti?c and administrative consultation.
关键词： funding opportunities,?uorescence imaging,light engines,light source
更新于2025-09-23 15:23:52
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Homo-molecular Fluorescence Complementation for Direct Visualization of Receptor Oligomerization in Living Cells
摘要： Cell surface receptor oligomerization is an attractive target process for drug screening. However, simple but reliable (thus high-throughput) visualization methods for receptor oligomerization are still lacking. Here, we report on a new single-construct Homo-molecular Fluorescence Complementation (Homo-FC) probe, which shows strong fluorescence signals by oligomerization of fused receptors in living cells with unexpectedly low background signals. Importantly, this high signal-to-noise ratio was not affected by expression level variations of fused receptors. The Homo-FC probe was developed by optimized flopped fusion of split fragments of superfolder green fluorescence protein and subsequent surface charge engineering. Homo-FC reliably visualized oligomerization of diverse natural receptors such as GPCR, EGFR, and even cytosolic DAI.
关键词： membrane receptor oligomerization,drug discovery,imaging,protein engineering,fluorescence complementation
更新于2025-09-23 15:23:52
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Diketopyrrolopyrrole Bisphosphonate Conjugate: A New Fluorescent Probe for in Vitro Bone Imaging
摘要： We report the synthesis of a conjugate molecule between an unusual red fluorescent diketopyrrolopyrrole (DPP) unit and a bisphosphonate (BP) precursor using the 'Click Chemistry' strategy to target bone tissue and monitor the interaction. After deep investigation, conjugation through a triazole unit between γ-azido rather than β-azido BP and an alkyne functionalized DPP fluorophore group turned out to be the winning strategy. Visualization of the DPP-BP conjugate on osteoclasts as well as the specific anti-resorption activity was successfully demonstrated.
关键词： fluorescent probe,in vitro imaging,osteoporosis,bisphosphonate,diketopyrrolopyrrole
更新于2025-09-23 15:23:52
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Experimental Realization of Multiple Topological Edge States in a 1D Photonic Lattice
摘要： Topological photonic systems offer light transport that is robust against defects and disorder, promising a new generation of chip-scale photonic devices and facilitating energy-efficient on-chip information routing and processing. However, present quasi one dimensional (1D) designs, such as the Su–Schrieffer–Heeger and Rice–Mele models, support only a limited number of nontrivial phases due to restrictions on dispersion band engineering. Here, a flexible topological photonic lattice on a silicon photonic platform is experimentally demonstrated that realizes multiple topologically nontrivial dispersion bands. By suitably setting the couplings between the 1D waveguides, different lattices can exhibit the transition between multiple different topological phases and allow the independent realization of the corresponding edge states. Heterodyne measurements clearly reveal the ultrafast transport dynamics of the edge states in different phases at a femtosecond scale, validating the designed topological features. The study equips topological models with enriched edge dynamics and considerably expands the scope to engineer unique topological features into photonic, acoustic, and atomic systems.
关键词： multi-topological numbers,topological photonics,topological edge states,ultra-fast heterodyne imaging
更新于2025-09-23 15:23:52
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Application of Visible-near Infrared Spectral Imaging for Monitoring Biological Materials
摘要： N ear infrared (NIR) spectroscopy is a powerful tool for the non-destructive evaluation of biological materials due to its generally weak absorption bands. Biological materials such as wood and plant leaves have a complicated structure in which the distribution of chemical composition and surface structure is non-uniform. Therefore, an imaging technique which combines high spatial resolution with the ability to acquire signal from a wider sample area is required. Three-dimensional image data such as hyperspectral imagery or a movie file has plenty of both spectral and spatial information. However, the visible-near infrared (vis-NIR) spectrum and the time profile of a single pixel normally display strong multicollinearity, thus requiring multivariate analysis for effective extraction of valuable information from three-dimensional image data. This article introduces two examples of image analysis for the non-destructive monitoring of biological materials.
关键词： spectroscopy,multivariate analysis,NIR,imaging,biological materials,hyperspectral,non-destructive evaluation,infrared
更新于2025-09-23 15:23:52
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The ablative fractional coagulation zone influences skin fluorescence intensities of topically applied test molecules-An in vitro study with fluorescence microscopy and fluorescence confocal microscopy
摘要： Background: Ablative fractional laser (AFL) increases uptake of topically applied skin agents. The coagulation zone (CZ) surrounding vertically ablated channels may influence uptake of drugs. Objectives: To investigate impact of CZ thickness on skin fluorescence intensities (FI) of a hydrophilic molecule by means of fluorescence microscopy (FM) and fluorescence confocal microscopy (FCM). Second, to compare FI of hydrophilic and lipophilic test molecules by FCM. Study Design/Methods and Materials: Microchannels with CZ thicknesses of 0, 20, and 80 μm were generated by microneedles or AFL (10,600 nm). Channels were 700 μm deep and number of channels kept constant per skin area. After 4 hours of incubation, FI induced by sodium fluorescein (NAF, hydrophilic, logarithmic partition-coefficient (logP) = -1.52, MW = 376.26) were quantified in both CZ and surrounding skin by FM (0–1,500 μm) and FCM (0–90 μm). FI of NAF and carboxyfluorescein (CAF, lipophilic, logP = 2.9, MW = 376.32) were compared by FCM. Results: By FM, NAF-induced FI were higher in CZ than in surrounding skin (P ≤ 0.001). Highest NAF-FI were induced in skin pretreated with a thin CZ (CZ–20 μm), assessed by both FM and FCM and in particular, FI were higher than in skin pretreated with no CZ (CZ–0 μm) (FM P ≤ 0.041, FCM P < 0.012). Skin FI remained constant to a depth of 500 μm, which corresponded to approximate depth of microchannels (CZ–0 μm, CZ–20 μm, CZ–80 μm: 0–500 μm P ≥ 0.107). In accordance with FM data, FCM showed higher FI within CZ than in surrounding skin, but gradually decreased to zero at a depth of 90 μm. NAF-FI were higher than CAF-FI (P ≤ 0.036), and highest CAF-FI were induced by CZ–0 μm and CZ–20 μm compared to CZ–80 μm (P ≤ 0.009). Conclusions: The influence of the CZ thickness on skin FI differs between small hydrophilic and lipophilic test molecules. Results may have clinical relevance for laser-assisted drug delivery.
关键词： sodium fluorescein,reflectance,carboxyfluorescein,dermatology,drug delivery,skin imaging
更新于2025-09-23 15:23:52
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Imaging multiple Rydberg wave packets from shaper-generated two-color femtosecond pump-probe sequences
摘要： We combine a bichromatic white light polarization pulse shaping scheme with angle- and energy-resolved photoelectron spectroscopy to image the dynamics of atomic Rydberg wave packets. Background-free detection of the Rydberg dynamics is performed using shaper-generated bichromatic linearly and circularly polarized femtosecond pump-probe pulse sequences. Photoelectron momentum distributions from linearly polarized bichromatic fields feature pronounced time-, energy-, and angle-dependent dynamics, which result from the interference of s-, d-, and g-type photoelectron wave packets from the ionization of the Rydberg np and nf series. Detailed analysis of the highly differential data allows us to extract the dynamics of both Rydberg wave packets separately. The results are experimentally verified by additional pump-probe studies with bichromatic circularly polarized pulse sequences, which exclusively map the dynamics of the Rydberg nf series via g-type photoelectron wave packets.
关键词： Rydberg wave packets,pump-probe spectroscopy,photoelectron imaging,femtosecond lasers,polarization pulse shaping
更新于2025-09-23 15:23:52
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Three-dimensional holographic imaging using data collected over cylindrical apertures
摘要： The previously proposed near-field holographic imaging algorithms are based on the data collected over rectangular apertures. In this letter, we propose an approach for three-dimensional near-field holographic imaging with data collected over cylindrical apertures. Circular deconvolution is employed to deal with the periodicity of the functions along the azimuthal direction.
关键词： holography,microwave imaging,non-destructive testing
更新于2025-09-23 15:23:52