- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Nonlinear optical microscopy for label-freely detecting gastric intestinal metaplasia
摘要： Intestinal metaplasia has been widely considered as a precursor of gastric cancer. As a result, early detection and accurate diagnosis will have important clinical significance. Therefore, nonlinear optical microscopy using two-photon excited fluorescence combined with second harmonic generation was used for investigating gastric mucosal intestinal metaplasia in this work. The imaging results showed that this microscope has the ability to directly identify this lesion from normal tissues in the absence of labels, and to further differentiate the subtyping of intestinal metaplasia. Furthermore, nuclear areas from normal gastric mucosa and intestinal metaplasia subtypes were measured, respectively, to show the cell changes while gastric mucosa developed from normal to intestinal metaplasia. This study may provide an approach for further researching these diseases in the future, and highlights the potential of nonlinear optical microscopy as a diagnostic tool for label-free identification of gastric intestinal metaplasia.
关键词： second harmonic generation,gastric intestinal metaplasia,nonlinear optical microscopy,two-photon excited fluorescence
更新于2025-09-10 09:29:36
-
Scan-Less, Kilo-Pixel, Line-Field Confocal Phase Imaging with Spectrally Encoded Dual-Comb Microscopy
摘要： Confocal laser microscopy (CLM) is a powerful tool in life science research and industrial inspection, and its image acquisition rate is boosted by scan-less imaging techniques. However, optical-intensity-based image contrast in CLM makes it difficult to visualize transparent non-fluorescent objects or reflective objects with nanometer unevenness. In this paper, we introduce an optical frequency comb (OFC) to scan-less CLM to give optical-phase-based image contrast. One-dimensional (1-D) image pixels of a sample are separately encoded onto OFC modes via 1-D spectral encoding by using OFC as an optical carrier of amplitude and phase with a vast number of discrete frequency channels. Then, line-field confocal information of amplitude and phase are decoded from mode-resolved OFC amplitude and phase spectra obtained by dual-comb spectroscopy. The proposed confocal phase imaging will further expand the applications of CLM.
关键词： optical interferometry,optical microscopy,Biomedical imaging
更新于2025-09-09 09:28:46
-
A 128-Pixel System-on-a-Chip for Real-Time Super-Resolution Terahertz Near-Field Imaging
摘要： This paper presents a fully integrated system-on-a-chip for real-time terahertz super-resolution near-field imaging. The chip consists of 128 sensing pixels with individual cross-bridged double 3-D split-ring resonators arranged in a 3.2 mm long 2 × 64 1-D array. It is implemented in 0.13-μm SiGe bipolar complementary metal–oxide–semiconductor technology and operated at around 550 GHz. All the functions, including sensor illumination, near-field sensing, and detection, are co-integrated with a readout integrated circuit for real-time image acquisition. The pixels exhibit a permittivity-based imaging contrast with a worst case estimated relative permittivity uncertainty of 0.33 and 10–12-μm spatial resolution. The sensor illumination is provided with on-chip oscillators feeding four-way equal power divider networks to enable an effective pixel pitch of 25 μm and a dense fill factor of 48% for the 1-D sensing area. The oscillators are equipped with electronic chopping to avoid 1/f-noise-related desensitization for the SiGe-heterojunction bipolar transistor power detectors integrated at each pixel. The chip features both an analog readout mode and a lock-in-amplifier-based digital readout mode. In the analog readout mode, the measured dynamic range (DR) is 63.8 dB for a 1-ms integration time at an external lock-in amplifier. The digital readout mode achieves a DR of 38.5 dB at 28 f/s. The chip consumes 37–104 mW of power and is packaged into a compact imaging module. This paper further demonstrates real-time acquisition of 2-D terahertz super-resolution images of a nickel mesh with 50-μm feature size, as well as a biometric human fingerprint.
关键词： terahertz,system-on-a-chip (SoC),SiGe heterojunction bipolar transistor (HBT),split-ring resonator (SRR),near-field array,3-push Colpitts oscillator,super-resolution imaging,near-field scanning optical microscopy (NSOM),power detector
更新于2025-09-09 09:28:46
-
A machine learning approach for online automated optimization of super-resolution optical microscopy
摘要： Traditional approaches for finding well-performing parameterizations of complex imaging systems, such as super-resolution microscopes rely on an extensive exploration phase over the illumination and acquisition settings, prior to the imaging task. This strategy suffers from several issues: it requires a large amount of parameter configurations to be evaluated, it leads to discrepancies between well-performing parameters in the exploration phase and imaging task, and it results in a waste of time and resources given that optimization and final imaging tasks are conducted separately. Here we show that a fully automated, machine learning-based system can conduct imaging parameter optimization toward a trade-off between several objectives, simultaneously to the imaging task. Its potential is highlighted on various imaging tasks, such as live-cell and multicolor imaging and multimodal optimization. This online optimization routine can be integrated to various imaging systems to increase accessibility, optimize performance and improve overall imaging quality.
关键词： machine learning,multicolor imaging,online automated optimization,live-cell imaging,super-resolution optical microscopy,multimodal optimization
更新于2025-09-04 15:30:14
-
Nanoapertures without nanolithography
摘要： We propose and experimentally demonstrate the implementation of lithography-free nano-apertures on optical fibers. By sputtering metallic nanofilms onto the end face of step index fibers that contain central nano-channels, fiber-integrated nano-apertures are instantaneously implemented without the use of any kind of lithographic step. In accordance with simulations, the experiments show diffraction-limited nano-spots in the far-field at the location of the nano-aperture for sufficiently thick films. We reproducibly implement a series of devices by sputtering Al and Pt nano-films, reaching aperture diameters as small as 40 nm and showing spectrally broadband operation. Due to its simplicity, scalability and potential for large-scale production the nano-aperture enhanced fiber concept will be highly relevant for lab-on-a-fiber applications and for the development of future fiber-based nano-probes with high spatial resolutions.
关键词： lab-on-a-fiber,hybrid fibers,nanofabrication,scanning near-field optical microscopy (SNOM),nanoapertures
更新于2025-09-04 15:30:14