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Fluorescence enhancement of a microbial rhodopsin via electronic reprogramming
摘要： The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly ten-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multi-configurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (sub-picosecond) fluorescence enhancement.
关键词： fluorescence enhancement,multi-configurational quantum chemistry,transient absorption spectroscopy,optogenetics,electronic reprogramming,microbial rhodopsins
更新于2025-09-04 15:30:14
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Fully implantable optoelectronic systems for battery-free, multimodal operation in neuroscience research
摘要： Recently developed ultrasmall, fully implantable devices for optogenetic neuromodulation eliminate the physical tethers associated with conventional set-ups and avoid the bulky head-stages and batteries found in alternative wireless technologies. The resulting systems allow behavioural studies without motion constraints and enable experiments in a range of environments and contexts, such as social interactions. However, these devices are purely passive in their electronic design, thereby precluding any form of active control or programmability; independent operation of multiple devices, or of multiple active components in a single device, is, in particular, impossible. Here we report optoelectronic systems that, through developments in integrated circuit and antenna design, provide low-power operation, and position- and angle-independent wireless power harvesting, with full user-programmability over individual devices and collections of them. Furthermore, these integrated platforms have sizes and weights that are not significantly larger than those of previous, passive systems. Our results qualitatively expand options in output stabilization, intensity control and multimodal operation, with broad potential applications in neuroscience research and, in particular, the precise dissection of neural circuit function during unconstrained behavioural studies.
关键词： wireless,optogenetics,neuroscience,optoelectronic systems,implantable
更新于2025-09-04 15:30:14
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Omnidirectional optogenetic stimulation
摘要： Omnidirectional optogenetic stimulation. A wireless, low-power optoelectronic platform, which is based on micro-LEDs, can provide multimodal programmable control over optogenetic stimulation parameters. Optogenetics is a powerful tool for perturbing populations of specific cell types. Here, individual cell types of the central and peripheral nervous systems are tagged with light-sensitive opsins. Specific wavelengths of light can then be used to turn the cells off and on, allowing complex neural circuitry to be dissected. For example, blue light can be used to activate neurons and green light can be used to deactivate neurons depending on the expression of either channelrhodopsin-2 (ChR) or halorhodopsins (Halo), respectively. The technique has been important in elucidating new neural pathways in many preclinical models of disease (including those related to memory, depression, sleep, anxiety, and restoration of vision) and could lead to potential new disease targets.
关键词： wireless,micro-LEDs,optogenetics,disease models,neural circuitry
更新于2025-09-04 15:30:14