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Assessing UV Inactivation of Adenovirus 41 Using Integrated Cell Culture Real-Time qPCR/RT-qPCR
摘要： Enteric adenoviruses are among most UV-resistant viruses in water. Cytopathic effects (CPE)-based cell culture TCID50 assay as a conventional virus assessment approach has major drawbacks for enteric adenovirus since it is selective on cell lines and takes longer time to show CPE. Integrated cell culture real-time quantitative PCR (ICC-qPCR) and reverse transcriptase (RT)-qPCR were applied in this study, in comparison with TCID50, to assess UV inactivation of adenovirus type 41 (Ad41) in water. Adenovirus type 41 was exposed to UV doses of 40, 80, 160, and 320 mJ/cm2 using a collimated beam apparatus. There was no significant difference of inactivation at conducted UV doses between measurements using TCID50 assay and ICC-RT-qPCR. Both assays fitted the Chick–Watson model at 95% confidence level. The inactivation measured by ICC-qPCR did not fit the Chick–Watson model. In summary, ICC-RT-qPCR is the most appropriate alternate to CPE-based assay for assessing UV inactivation of enteric adenoviruses. Water Environ. Res., 89, 323 (2017).
关键词： cytopathic effects,cell culture,real-time quantitative PCR/RT-PCR,adenovirus type 41,water,UV inactivation
更新于2025-09-23 15:22:29
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Determination of Effective Dose of Ultraviolet Irradiation to Influent Water for Prevention of <i>Kudoa yasunagai</i> Infection
摘要： We determined the minimum ultraviolet (UV) irradiation dose to influent water to prevent Kudoa yasunagai infection, using microscopy and qPCR. The infection prevalence in Seriola lalandi reared in untreated water reached 45% while that in fish reared in UV-treated water at 5 mJ/cm2 remained below 10%. Additionally, 5 mJ/cm2 UV irradiation significantly reduced spore formation in the brain. No infection was detected when the water was treated with UV at the doses 15 and 30 mJ/cm2. These results indicate that K. yasunagai actinospores are relatively vulnerable to UV irradiation and the minimum effective dose lies between 5 and 15 mJ/cm2.
关键词： quantitative PCR,ultraviolet irradiation,Seriola lalandi,prevention,Kudoa yasunagai,hatchery
更新于2025-09-23 15:21:21