- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Multifunctional luminescent immuno-magnetic nanoparticles: toward fast, efficient, cell-friendly capture and recovery of circulating tumor cells
摘要： Highly efficient isolation and recovery of viable circulating tumor cells (CTCs) from the blood of patients is an important precondition to address the current dilemma of insufficient CTC studies, and can promote the development of individualized antitumor therapies. Herein, a cell-friendly CTC isolation and recovery nanoplatform with luminescent labelling was established using a layer-by-layer (LbL) assembly technique and a stimulated cellular-release strategy. In particular, the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody was introduced with a disulfide bond-containing linker for further bio-friendly recovery of the CTCs. Quantum dots (QDs) were deposited onto fast magnet-responsive Fe3O4 nanoparticles through a facile LbL assembly method to monitor the capture and recovery process in real time. The obtained PEGylated immuno-magnetic nanospheres (PIMNs) can all be magnetically collected within 2 min. Capture efficiencies above 90% can be achieved from blood samples with 5–200 CTCs per mL after only 1–2 min incubation. Nearly all PIMNs on the surface of the CTCs were detached after 15 min of glutathione (GSH) treatment with the disappearance of QD signals. Recovered CTCs could be directly used for culture (cell viability, 98%), and their invasiveness and migration characteristics remained unchanged. Furthermore, the PIMNs were successfully applied to isolate CTCs in cancer patients’ peripheral blood samples, and an average of 8.6 ± 5.8 CTCs per mL was detected. The results above suggested that PIMNs may serve as a powerful nanoplatform for CTC screening, isolation and recovery.
关键词： layer-by-layer assembly,circulating tumor cells,capture and recovery,Multifunctional luminescent immuno-magnetic nanoparticles,quantum dots,glutathione treatment
更新于2025-09-19 17:15:36
-
One-step scalable fluorescent microgel bioassay for the ultrasensitive detection of endogenous viral miR-US4-5p
摘要： Human cytomegalovirus (hCMV) infection is the leading cause of birth defects in newborns and death in immunosuppressed people. Traditional techniques require time-consuming and costly analyses, and sometimes result in false positive results; thus, a rapid and accurate detection for hCMV infection is necessary. Recently, hcmv-miR-US4-5p was selected as the biomarker for cytomegalovirus diagnosis and follow-up. Herein, we propose a bioassay based on microgels endowed with optical fluorescent oligonucleotide probes for the detection of circulating endogenous hcmv-microRNAs. In particular, a double strand probe, based on the fluorescence recovery after target capture, was conjugated on microgels and the probe density was opportunely optimised. Then, the microgels were directly mixed with the sample. The fluorescence read-out was measured as a function of target concentration at a fixed number of microgels per tube. As a bead-based assay, the performances of optical detection in terms of dynamic working range and limit of detection could be finely tuned by tuning the number of microgels per tube. The limit of detection of the assay could be tuned in the range from 39.1 fM to 156 aM by changing the microgel concentration from 50 μg mL?1 to 0.5 μg mL?1, respectively. The assay results specific for the selected target were stable over a one-year time span and they were not affected by the presence of human serum. Therefore, this bioassay based on microgels might represent a flexible platform that should be able to predict, identify and follow-up several diseases by monitoring freely circulating oligonucleotides in body fluids.
关键词： hcmv-miR-US4-5p,fluorescence recovery,toehold displacement,microgel bioassay,ultrasensitive detection
更新于2025-09-19 17:15:36
-
Improved electro-optic chaotic system with nonlinear electrical coupling
摘要： Design disjunction is developed to offer a broad coverage, high resolution, and low overhead approach to online diagnosis and recovery of recon?gurable fabrics. Design disjunction leverages the condensed diagnosability of T logic resources to achieve self-recovery using partial recon?guration in O(log T ) steps. Recon?guration is guided by the constructive property of f-disjunctness which forms O(log T ) resource groups at design-time. Resolution of f simultaneous resource faults is shown to be guaranteed when the resource groups are mutually f-disjunct. This extends run-time fault resilience to a large resource space with certainty for up to f faults using a decision-free resolution process that also provides a high likelihood of identifying the fault’s location to a ?ne granularity. Finally, design disjunction is parameterized to accommodate the low coverage issue of functional testing for which inarticulate tests can otherwise impair fault isolation. Experimental results for MCNC and ISCAS benchmarks on a Xilinx 7-series ?eld programmable gate array (FPGA) demonstrate f-diagnosability at the individual slice level with a minimum average isolation accuracy of 96:4 percent (94:4 percent) for f ? 1 (f ? 2). Results have also demonstrated millisecond order recovery with a minimum increase of 83:6 percent in fault coverage compared to N-modular redundancy (NMR) schemes. Recovery is achieved while incurring an average critical path delay impact of only 1:49 percent and energy cost roughly comparable to conventional two-MR approaches.
关键词： online test,run-time fault diagnosis and recovery,design space exploration,Recon?gurable logic devices,autonomous fault handling,fault-tolerant systems,?eld programmable gate arrays
更新于2025-09-19 17:13:59
-
[IEEE 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Chicago, IL, USA (2019.6.16-2019.6.21)] 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Measured dc Arc-flash Risk in a Photovoltaic System
摘要： In this paper, the energy recovery in microstrip passive circuits from the power losses into heat is studied. For this purpose, a thermoelectric generator (TEG) based on the Seebeck effect principle is used, which converts part of the power dissipated into heat to dc electrical power. A solution integrating the TEG with the microstrip circuit is proposed, and design guidelines in order to optimize the recovered power keeping a good isolation between the RF signal and the TEG system are provided. As will be shown, under moderate applied signal powers of just 1–5 W, the levels of recovered power in microstrip passive circuits can be notable. As a demonstrator circuit, an integration device formed by an embedded microstrip bandpass filter for WiMAX applications and a TEG is designed, fabricated, and characterized (thermal and electrically). Different scenarios are considered, depending on frequency and thermal loads. For an applied in-band CW input signal power of 2 W at 3.48 GHz, a recovered power of around 250 μW has been continuously supplied to the electrical load. Several aspects, such as efficiency and future improvements, are also discussed.
关键词： power applications,Average power handling capability (APHC),microwave devices,planar circuits,energy recovery,electro-thermal analysis,energy harvesting
更新于2025-09-19 17:13:59
-
Implementation of Metallic Vertical Interconnect Access in Hybrid Intercalated Graphene/Quantum Dot Photodetector for Improved Charge Collection
摘要： Performance improvement of heat recovery-based power generation (HRPG) systems puzzles researchers due to the implicitly coupled property. This contribution builds the heat current model of a HRPG system and applies the circuit principle to derive its heat transfer and conversion constraints. Then, derivation of the flow resistance balance equations describes the pressure distribution in circulation loop. Combining with the relations between fluid temperatures and pressures constructs the integrated system model. Based on the separation of linear topology equations and explicit nonlinear component equations from implicit nonlinear constraints using heat current model, this contribution introduces a fast and stable divide-and-conquer solution scheme, which solves linear equations in iterations and updates the iterative variables by substitutions. Finally, optimization of the fluid flow rates for the maximum net electricity generation under different working conditions shows the superiority of the heat current method over the commercial software Ebsilon. The optimized results show that a smaller mass flow rate of flue gas leads to lower optimal evaporation and condensation pressures, while the optimal dryness fraction of exhaust steam is almost unchanged. Besides, the constant backpressure operation strategy apparently deviates from the optima, and the maximum deviation reaches 8.6%.
关键词： Energy conservation,Heat recovery-based power generation,Solution scheme,Heat current model,Global optimization
更新于2025-09-19 17:13:59
-
[IEEE 2019 Photonics & Electromagnetics Research Symposium - Fall (PIERS - Fall) - Xiamen, China (2019.12.17-2019.12.20)] 2019 Photonics & Electromagnetics Research Symposium - Fall (PIERS - Fall) - Coordinate-free Formulation and Evaluation of Tensor Greena??s Functions for General Homogeneous Uniaxial Anisotropic Media
摘要： Stained glass windows are designed to reveal their powerful artistry under diverse and time-varying lighting conditions; virtual relighting of stained glass, therefore, represents an exceptional tool for the appreciation of this age old art form. However, as opposed to most other artifacts, stained glass windows are extremely difficult if not impossible to analyze using controlled illumination because of their size and position. In this paper, we present novel methods built upon image based priors to perform virtual relighting of stained glass artwork by acquiring the actual light transport properties of a given artifact. In a preprocessing step, we build a material-dependent dictionary for light transport by studying the scattering properties of glass samples in a laboratory setup. We can now use the dictionary to recover a light transport matrix in two ways: under controlled illuminations the dictionary constitutes a sparsifying basis for a compressive sensing acquisition, while in the case of uncontrolled illuminations the dictionary is used to perform sparse regularization. The proposed basis preserves volume impurities and we show that the retrieved light transport matrix is heterogeneous, as in the case of real world objects. We present the rendering results of several stained glass artifacts, including the Rose Window of the Cathedral of Lausanne, digitized using the presented methods.
关键词： light transport,recovery,dictionary learning,Banded matrices,stained glass,sparse cultural artifacts,computational relighting
更新于2025-09-19 17:13:59
-
[IEEE 2019 International Conference on Innovative Sustainable Computational Technologies (CISCT) - Dehradun, Uttarakhand, India (2019.10.11-2019.10.12)] 2019 International Conference on Innovative Sustainable Computational Technologies (CISCT) - Low Cost Laser Light Security System in Smart Home
摘要： A traf?c matrix is generally used by several network management tasks in a data center network, such as traf?c engineering and anomaly detection. It gives a ?ow-level view of the network traf?c volume. Despite the explicit importance of the traf?c matrix, it is signi?cantly dif?cult to implement a large-scale measurement to build an absolute traf?c matrix. Generally, the traf?c matrix obtained by the operators is imperfect, i.e., some traf?c data may be lost. Hence, we focus on the problems of recovering these missing traf?c data in this paper. To recover these missing traf?c data, we propose the spatio-temporal Kronecker compressive sensing method, which draws on Kronecker compressive sensing. In our method, we account for the spatial and temporal properties of the traf?c matrix to construct a sparsifying basis that can sparsely represent the traf?c matrix. Simultaneously, we consider the low-rank property of the traf?c matrix and propose a novel recovery model. We ?nally assess the estimation error of the proposed method by recovering real traf?c.
关键词： Traf?c matrix recovery,network management,network measurement,matrix completion,Kronecker compressive sensing
更新于2025-09-19 17:13:59
-
[IEEE 2019 IEEE 8th International Workshop on Computational Advances in Multi-Sensor Adaptive Processing (CAMSAP) - Le gosier, Guadeloupe (2019.12.15-2019.12.18)] 2019 IEEE 8th International Workshop on Computational Advances in Multi-Sensor Adaptive Processing (CAMSAP) - Is There Any Recovery Guarantee with Coupled Structured Matrix Factorization for Hyperspectral Super-Resolution?
摘要： Coupled structured matrix factorization (CoSMF) for hyperspectral super-resolution (HSR) has recently drawn significant interest in hyperspectral imaging for remote sensing. Presently there are very few studies on the theoretical recovery guarantees of CoSMF. This paper makes one such endeavor by considering the CoSMF formulation by Wei et al., which, simply speaking, is similar to coupled non-negative matrix factorization. Assuming no noise, we show sufficient conditions under which the globally optimal solution to the CoSMF problem is guaranteed to deliver certain recovery accuracies. Our analysis suggests that sparsity and the pure-pixel (or separability) condition play a hidden role in enabling CoSMF to achieve some good recovery characteristics.
关键词： coupled structured matrix factorization,hyperspectral super-resolution,recovery guarantee
更新于2025-09-19 17:13:59
-
Investigation into the current loss in InAs/GaAs quantum dot solar cells with Si-doped quantum dots
摘要： Our previous studies have shown that introducing Si doping in quantum dots (QDs) can help QD solar cells achieve higher voltage. However, this improvement came at the cost of current loss. In this work, we continue to investigate the cause of the current loss and propose a method to recover it without compromising the voltage. Photoluminescence measurements have confirmed that optimizing the thickness of the GaAs layers in the i-region can lead to strong current gain (~14%) with minimal voltage loss (<3%) and alteration of the QD quality. The capacitance–voltage measurement results support that the current gain mainly originates from the increased depletion width.
关键词： MBE,current recovery,QDSC
更新于2025-09-19 17:13:59
-
[IEEE 2019 18th International Conference on Optical Communications and Networks (ICOCN) - Huangshan, China (2019.8.5-2019.8.8)] 2019 18th International Conference on Optical Communications and Networks (ICOCN) - Clock Recovery Based on Adaptive Phase Error Detection for Coherent Optical Transmission Systems
摘要： Traditional clock recovery techniques rely on Gardner for phase error detection to recover the original signal. An adaptive phase splitting detection technique is proposed by joint channel equalization in this paper. The loop judges the current error by the error of the previous moment, reduces the phase error jitter, and improves the stability of the system. In addition, the algorithm works well for the same phase of adjacent symbols through phase splitting techniques. In fiber channel, this scheme is more suitable for the case of small roll-off factor (ROF). Simulation results show that the algorithm is suitable for signals modulated by MQAM. The algorithm performs very well even when the ROF approaches 0.
关键词： Adaptive phase error detection,Phase splitting timing recovery,Channel equalization,Coherent optical communication
更新于2025-09-19 17:13:59