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Silanized quantum dots as labels in lateral flow test strips for C-reactive protein
摘要： The paper describes the first use of silanized semiconductor core-shell quantum dots as fluorescent labels for macromolecule, C-reactive protein determination in blood plasma. The controlled synthesis of CdSe cores, with successive shells of CdS, CdZnS, ZnS and coating with transparent, stable, and inert silica shell, provides quantum dots with a narrow emission band, high quantum yield, and prolonged signal stability. Finally, the quantum dots were conjugated with specific antibodies via carboxylic groups on the silica surface. The method was further used for the immunochromatographic assay of C-reactive protein, a diagnostically important inflammatory biomarker. Assays with both the fluorescent QDs and a widely used colloidal gold label were developed in parallel and compared. The silanized quantum dots provide a more sensitive assay with a detection limit of 1 ng/mL for C-reactive protein in standard solutions, whereas the common assay has a detection limit of 10 ng/mL. The possibility of quantitative evaluation of analyte content by a portable device was demonstrated; the accuracy of the measurements was in the range of 5%–10%. The tests were used to determine C-reactive proteins in human plasma samples. The selected optimized protocol for these samples is based on a 4-fold dilution. The final working range of the assay, 4–1,200 ng/mL, covers practically all important interval of C-reactive protein values for the characterization of acute, chronic, and local inflammatory processes. Due to their high physical stability and inertness as well as intense, stable, and reproducible fluorescence, silanized quantum dots may be applied for high-sensitive assays for different analytes.
关键词： C-reactive protein,Quantum dots,silanization,lateral flow immunoassay
更新于2025-11-19 16:46:39
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Fabrication of controllable wettability of crystalline silicon surfaces by laser surface texturing and silanization
摘要： This paper aims to explore the e?ects of laser surface texturing and chemical modi?cations by silanization on the wettability of silicon surfaces. To e?ectively prepare super-hydrophobic surfaces on silicon, a process using laser texturing with following silanization treatment is used to modify the surface. The surface wettability is evaluated by water contact angle (WCA). To ascertain the e?ect of laser texturing on wettability, the morphological and metallurgical modi?cations of the surface were investigated by scanning electron microscopy (SEM), white light confocal microscope and X-ray photoelectron spectroscopy (XPS). It is found that the fresh laser-processed silicon surface is super-hydrophilic, which is in line with the Wenzel model. A super-hydrophobic surface with a WCA > 150° was successfully obtained after silanization treatment. Silanization by a concentration of 0.72 wt% 1H, 1H, 2H, 2H-per?uorodecyltrichlorosilane with 2 h of immersion time was found to be the most e?ective condition for achieving super-hydrophobic silicon surfaces. The e?ects of acids, alkalis and salts on wettability have also been assessed. The results indicate that as-prepared silicon samples with a ?uoride coating can be normally used in acid condition but not suitable for use in alkaline and salty environments.
关键词： Laser texturing,Wettability,Silanization,Alkalinity,Silicon,Acidity,Salinity
更新于2025-09-16 10:30:52
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Hybridization of graphene oxide into nanogels to acquire higher photothemal effects for therapeutic delivery
摘要： Although the special architecture of two-dimensional (2D) nanomaterials endows them with unique properties, their poor colloidal stability remains a main bottleneck to fully exploit their applications in the biomedical field. Herein, this study aims to develop a simple and effective approach to in situ incorporate 2D graphene oxide (GO) nanopletlets into thermosensitive matrix to acquire hybrid nanogels with good stability and photothermal effect. In order to improve its stability, GO firstly underwent silanization to its surface with double bonds, followed by intercalation with N-isopropylacrylamide (NIPAM) in the presence of disulfide-containing crosslinker via an emulsion method. Radical polymerization was then initiated to accelerate direct GO exfoliation in PNIPAM nanogels by forming covalent bonds between them. The well-dispersed GO nanopletlets in the nanogels not only displayed an enhanced photothermal effect, but also improved the encapsulation efficiency of an anticancer drug. The hybrid nanogels accelerate drug release under conditions mimicking the acidic/reducible solid tumor and intracellular microenvironments, most importantly, it can be further enhanced via remote photothermal treatment. The multifunctional nanogels potentiate their synergistic anticancer bioactivity as an ideal nanoplatform for cancer treatment.
关键词： Graphene Oxide,Anticancer Drug Delivery,Photothermal Effect,Silanization,Thermosensitive Nanogels
更新于2025-09-04 15:30:14