- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Photodynamic therapy in infected venous and mixed leg ulcers: a pilot experience
摘要： Objective: To assess the tolerability and efficacy of photodynamic therapy (PDT) with RLP068, a novel phthalocyanine-derived photosensitiser, in controlling the bacterial load in different leg ulcers, due to vascular pathophysiology. Method: An observational study of patients with infected leg ulcers of different pathophysiology, admitted to the hospital for a skin grafting procedure, were included. All patients underwent two sessions of PDT at time zero and after 72 hours. A semi-quantitative swab was taken before and 30 minutes after both sessions of PTD. During the time interval between the two treatments the ulcer was covered with foam dressing and compression therapy was applied to all patients. No systemic or topical antibiotics were administered. Statistical analysis of results was performed. Results: A total of 36 patients (13 males, 23 females; aged 72.4±8.6 years, range: 55-85 years) were recruited; two had ulcers on both legs. PDT was shown to be effective in reducing bacterial load after the first treatment. Before the second PDT treatment (72 hours after first PDT treatment), a slight increase of the bacterial load was observed in all ulcers. However, after the second PDT session, bacterial swab results were negative in all but two ulcers. The procedure was well tolerated in all but four patients, who reported a very severe pain at baseline, which increased during treatment. Conclusion: In this study, PDT was effective in reducing bacterial load in patients with infected vascular leg ulcers, and allowed successful skin grafting to take place in all patients. The treatment was generally well tolerated. Studies with a greater number of patients and a control group are planned to confirm these results.
关键词： infected ulcers,phthalocyanine,vascular ulcers,photodynamic therapy,skin grafting
更新于2025-09-04 15:30:14
-
[IEEE 2018 43rd International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz 2018) - Nagoya (2018.9.9-2018.9.14)] 2018 43rd International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz) - Experimental Characterization of Artificial Human Skin with Melanomas for Accurate Modelling and Detection in Healthcare Application
摘要： A preliminary investigation is carried out on the artificial human skin tissues with and without metastatic melanomas using Terahertz Time Domain Spectroscopy (THz-TDS). Both the refractive indexes and absorption coefficients of artificial skin with melanomas are higher than the normal artificial skin samples over the entire frequency range between 0.2 THz to 1.6 THz. The reason is that tumour cells degrade the in contraction of fibroblasts causing more water content malignant tissues. This study quantifies the impact of melanomas on the optical parameters of artificial skin tissue and can help in techniques that will diagnose and prevent tumours at the early stage.
关键词： artificial human skin,absorption coefficient,Terahertz Time Domain Spectroscopy,refractive index,melanomas
更新于2025-09-04 15:30:14
-
Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents- quantitative analysis using confocal Raman microscopy
摘要： Confocal Raman microscopy (CRM) has been used to measure depth-dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque? (300)) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 min. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 min), 33.1% for glycerol (30 min), 8.3% for Omnipaque? (60 min), and 4.4% for Omnipaque? (30 min), on average for the 40–200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5%, and 4.6%), strongly bound (50.4%, 33.0%, 7.9%, and 3.4%), tightly bound (63.6%, 42.3%, 26.1%, and 12.9%) and unbound (55.4%, 28.7%, 10.1%, and 5.9%) water types on average for the 40–200 μm depths, post application of glycerol (60 min), glycerol (30 min), Omnipaque? (60 min), and Omnipaque? (30 min), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA-induced water flux in the skin, and thus, are responsible for optical clearing efficiency.
关键词： dermis,glycerol,skin barrier,Omnipaque?,epidermis,iohexol,Raman spectroscopy
更新于2025-09-04 15:30:14
-
Spectrophotometric Measurement of Minimal Erythema Dose Sites after Narrowband Ultraviolet B Phototesting: Clinical Implication of Spetrophotometric Values in Phototherapy
摘要： Background: The spectrophotometer is well known to be a useful tool for estimating the objective minimal erythema dose (MED) during planning of phototherapy protocol. However, only a few spectrophotometric values are used to evaluate the erythema and pigmentation of the MED site during phototesting. Objective: To determine a new meaning of the relationships among spectrophotometric values during phototesting. Methods: Twenty-five patients with psoriasis and 23 patients with vitiligo were selected before undergoing narrowband ultraviolet B phototherapy. We interpreted the gross findings of erythema and measured the L*a*b* values using a spectrophotometer at each phototest spot. We compared MEDs, basic spectrophotometric values (L*a*b*), and b*/L* values separately according to skin type, and determined the correlation of each spectrophotometric value and the correlation between a* and b*/L* values. Results: Among L*a*b* values, only b* values showed a statistically significant difference between the type III and IV groups (p=0.003). There was a positive correlation only between MEDs and b* values (p＜0.05). The average b*/L* value in the type IV group was significantly higher than the type III group (p＜0.05). Conclusion: The higher b* values in type IV skin indicates that skin tanning develops more prominently than type III. The correlation between MEDs and b* values may signify that the skin pigmentation status is deepened with the higher MEDs. The difference in b*/L* values between type III and IV skin reflects that the b*/L* value is thought to be an index of tanning. The a* value, known as an index of erythema, does not influence the degree of tanning.
关键词： a*value,Skin phototype,Spectrophotometer,L*value
更新于2025-09-04 15:30:14
-
Integrating an ex-vivo skin biointerface with electrochemical DNA biosensor for direct measurement of the protective effect of UV blocking agents
摘要： Skin cancer is the most frequent kind of cancer in white people in many parts of the world. UV-induced DNA damage and genetic mutation can subsequently lead to skin cancer. Therefore development of new biosensing strategies for detection of UV-induced DNA damage is of great importance. Here we demonstrate a novel combination of an ex-vivo skin biointerface and an electrochemical DNA sensor for the direct detection of UV induced DNA damage and investigation the protective effect of various UV blockers (Zinc-oxide (ZnO), titanium-dioxide (TiO2) nanoparticles (NPs) and sunscreens) against DNA damage. A diazonium modified screen-printed carbon electrode immobilized with a DNA sequence related to the p53 tumour suppressor gene, the most commonly affected gene in human UV-induced skin cancer, was applied as an electrochemical DNA sensor. Electrochemical impedance spectroscopy (EIS) was employed for the detection of DNA damage induced by UV-A radiation by following the changes in charge transfer resistance (Rct). The protective effects of UV blockers applied onto a pig skin surface (a suitable model representing human skin) were successfully detected by the DNA sensor. We observed that the naked skin has little UV protection showing an 18.2% decreases in ?R/R values compared to the control, while applying both NPs and NP-formulated sunscreens could significantly reduce DNA damage, resulting in a decrease in ?R/R values of 67.1% (ZnO NPs), 77.2% (TiO2 NPs), 77.1% (sunscreen 1) and 92.4% (sunscreen 2), respectively. Moreover, doping moisturising cream with NPs could provide a similar DNA protective effect. This new method is a biologically relevant alternative to animal testing and offers advantages such as fast, easy and inexpensive processing, in addition to its miniaturised dimension, and could be used for a range of applications in other sources of DNA damage and the protective effect of different UV blocking agents and other topical formulations.
关键词： sunscreens,electrochemical DNA biosensor,skin biointerface,DNA damage,nanoparticles
更新于2025-09-04 15:30:14