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Fast simultaneous image super-resolution and motion deblurring with decoupled cooperative learning
摘要： In recent years, deep convolutional neural networks (CNNs) have been widely applied to handle low-level vision problems. However, most existing CNN-based approaches can either handle single degeneration each time or treat them jointly through feature entangling, thus likely leading to poor performance when the actual degradation is inconsistent with hypothetical degradation condition. Furthermore, feature coupling will bring a large amount of computation, which may make the methods impractical to real-time mobile scenarios. In order to address these problems, we propose a deep decoupled cooperative learning model which can not only develop the corresponding recover network to deal with each degradation, but also flexibly handle multiple degradations at the same time. Thus, our approach can achieve disentangling and synthesizing single image super-resolution and motion deblurring, which has high practicability. We evaluate the proposed approach on various benchmark datasets, covering both natural images and synthetic images. The results demonstrate its superiority, compared to the state-of-the-art, where image SR and motion deblurring can be accomplished effectively concurrently. The source code of the work is available at https://github.com/hengliusky/Cooperative-Learning-Deblur-SR.
关键词： Motion deblurring,Image super-resolution,Decoupled cooperative learning
更新于2025-09-23 15:21:01
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A Polymerizable Photoswitchable Fluorophore for Super-Resolution Imaging of Polymer Self-Assembly and Dynamics
摘要： Single-molecule super-resolution microscopy has become a standard imaging tool in the life sciences for visualizing nanostructures in situ, but the application of this technique in polymer science is much less explored. A key bottleneck is the lack of fluorophores and simple covalent attachment strategies onto polymer chains. Here, we report a functional diarylethene-based photoswitchable fluorophore that can be directly incorporated into polymer backbones through copolymerization, which significantly streamlines the labeling strategy, with no further postcoupling reactions or purifications needed. The attachment of fluorophores onto selectively labeled polymers enables super-resolution imaging of a series of model polymer blend systems with different nanostructures and chemical compositions. As each individual fluorophore is able to switch several times on average between its bright and dark state, multiple time-lapse images can be acquired to observe the dynamic nanostructural evolution of polymer blends upon solvent vapor annealing. With this demonstration of a universal, simplified labeling strategy and the ability to image polymer assembly under native conditions, this reported fluorophore may promote the widespread use of super-resolution microscopy in the polymer community.
关键词： diarylethene,polymer dynamics,super-resolution microscopy,photoswitchable fluorophore,polymer self-assembly
更新于2025-09-23 15:21:01
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Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium <em>Prochlorococcus</em>
摘要： Super-resolution microscopy has been widely used to study protein interactions and subcellular structures in many organisms. In photosynthetic organisms, however, the lateral resolution of super-resolution imaging is only ~100 nm. The low resolution is mainly due to the high autofluorescence background of photosynthetic cells caused by high-intensity lasers that are required for super-resolution imaging, such as stochastic optical reconstruction microscopy (STORM). Here, we describe a photobleaching-assisted STORM method which was developed recently for imaging the marine picocyanobacterium Prochlorococcus. After photobleaching, the autofluorescence of Prochlorococcus is effectively reduced so that STORM can be performed with a lateral resolution of ~10 nm. Using this method, we acquire the in vivo three-dimensional (3-D) organization of the FtsZ protein and characterize four different FtsZ ring morphologies during the cell cycle of Prochlorococcus. The method we describe here might be adopted for the super-resolution imaging of other photosynthetic organisms.
关键词： Prochlorococcus,photobleaching,FtsZ ring,Immunology and Infection,STORM,cell division,cyanobacterium,super-resolution imaging,three-dimensional,Issue 141
更新于2025-09-23 15:21:01
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European Microscopy Congress 2016: Proceedings || New Opportunities in multi-frame STEM Spectroscopy & Fractional Beam-current EELS
摘要： The paper discusses the application of multi-frame super-resolution techniques in the field of optoelectronics, specifically focusing on the enhancement of image quality through the use of advanced algorithms and hardware. It explores the limitations and potential improvements in current methodologies, presenting a novel approach to image processing that leverages both software and hardware innovations.
关键词： hardware acceleration,super-resolution,image processing,multi-frame,optoelectronics
更新于2025-09-23 15:21:01
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Increasing fluorescence lifetime for resolution improvement in STED nanoscopy
摘要： Super-resolution microscopy (SRM) has had a substantial impact on the biological sciences due to its ability to observe tiny objects less than 200 nm in size. Stimulated emission depletion (STED) microscopy represents a major category of these SRM techniques that can achieve diffraction-unlimited resolution based on a purely optical modulation of fluorescence behaviors. Here, we investigated how the laser beams affect fluorescence lifetime in both confocal and STED imaging modes. The results showed that with increasing illumination time, the fluorescence lifetime in two kinds of fluorescent microspheres had an obvious change in STED imaging mode, compared that in confocal imaging mode. As a result, the reduction of saturation intensity induced by the increase of fluorescence lifetime can improve the STED imaging resolution at the same depletion power. The phenomenon was also observed in Star635P labeled human Nup153 in fixed HeLa cells, which can be treated as a reference for the synthesis of fluorescent labels with the sensitivity to the surrounding environment for resolution improvement in STED nanoscopy.
关键词： confocal microscopy,super-resolution,fluorescence lifetime,fluorescence microscopy
更新于2025-09-23 15:21:01
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In Vivo Confocal Imaging of Fluorescently Labelled Microbubbles: Implications for Ultrasound Localization Microscopy
摘要： We report the time kinetics of fluorescently labelled microbubbles in capillary-level microvasculature as measured via confocal microscopy and compare these results to ultrasound localization microscopy. The observed 19.4 ± 4.2 microbubbles per confocal field-of-view (212 μm x 212 μm) is in excellent agreement with the expected count of 19.1 microbubbles per frame. The estimated time to fully perfuse this capillary network was 193 seconds, which corroborates the values reported in literature. We then modeled the capillary network as an empirically determined discrete-time Markov chain with adjustable microbubble transition probabilities though individual capillaries. Monte Carlo random walk simulations found perfusion times ranging from 24.5 seconds for unbiased Markov chains up to 182 seconds for heterogeneous flow distributions. This pilot study confirms a probability-derived explanation for the long acquisition times required for super-resolution ultrasound localization microscopy.
关键词： Super-resolution,Ultrasound Microvessel Imaging,Microbubble tracking,Microvasculature
更新于2025-09-23 15:19:57
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Graphene-Enabled, Spatially Controlled Electroporation of Adherent Cells for Live-Cell Super-resolution Microscopy
摘要： The incorporation of exogenous molecules into live cells is essential for both biological research and therapeutic applications. In particular, for the emerging field of super-resolution microscopy of live mammalian cells, it remains a challenge to deliver tailored, often cell-impermeable, fluorescent probes into live cells for target labeling. Here, utilizing the outstanding mechanical, electrical, and optical properties of graphene, we report a facile approach that enables both high-throughput delivery of fluorescent probes into adherent mammalian cells and in situ super-resolution microscopy on the same device. ~90% delivery efficiencies are achieved for free dyes and dye-tagged affinity probes, short peptides, and whole antibodies, thus enabling high-quality super-resolution microscopy. Moreover, we demonstrate good spatiotemporal controls, which, in combination with the ready patternability of graphene, allow for the spatially selective delivery of two different probes for cells at different locations on the same substrate.
关键词： electroporation,graphene,intracellular delivery,live-cell labeling,super-resolution microscopy
更新于2025-09-23 15:19:57
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Microscope laser assisted photooxidative activation of bioorthogonal ClickOx probes
摘要： A photoactivatable fluorogenic tetrazine-rhodaphenothiazine probe was synthesized and studied in light-assisted, bioorthogonal labeling schemes. Experimental results revealed that the bioorthogonally conjugated probe efficiently sensitizes 1O2 generation upon illumination with green or orange light and undergoes self-oxidation leading to an intensely fluorescent sulfoxide product. An added value of the present probe is that it is also suitable for STED super-resolution microscopy using a 660 nm depletion laser.
关键词： tetrazine-rhodaphenothiazine,fluorogenic,STED,super-resolution microscopy,photoactivatable,bioorthogonal,labeling
更新于2025-09-23 15:19:57
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Electrochemical Polymerization: An Emerging Approach for Fabricating High-Quality Luminescent Films and Super-Resolution OLEDs
摘要： Currently, the fabrication of high-resolution OLEDs remains great limitations. Electrochemical polymerization is an efficient way to fabricate conducting polymer films. However, the control of the structures and dedoping of films is difficult, thus making the films rough and incapable with flat-panel devices. In this review, the molecular design and the fabrication of high-quality electrochemical polymerization films and super-resolution RGB displays are introduced. Inspired by the unique electrochemical features of N-alkyl carbazoles, highly luminescent precursors are designed with the spatially separated emissive cores and side carbazoles. The films with low doping levels, high luminescent efficiencies and smooth morphology are prepared by optimizing potential windows, electrolyte solutions, scan rates and post-washing process. The luminous efficiency of OLEDs where the films as light-emitting layers exceeds that of most OLEDs prepared by conventional methods. Then electrocopolymerization and multilayer electropolymerization techniques are developed, in which the components and thickness of films can be precisely controlled, endowing the films and OLEDs with superior stability. Finally, RGB active-matrix OLEDs and super-resolution displays of 2822 ppi are achieved. The above works demonstrate electrochemical polymerization is an emerging technique in RGB super-resolution displays and has great commercial potential.
关键词： OLEDs,Electrochemical polymerization,RGB,N-alkyl carbazoles,super-resolution displays
更新于2025-09-23 15:19:57
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Super-resolution imaging of self-assembled nanocarriers using quantitative spectroscopic analysis for cluster extraction
摘要： Self-assembled nanocarriers have inspired a range of applications for bioimaging, diagnostics, and drug delivery. Non-invasive visualization and characterization of nanocarriers are important for understanding their structure to function relationship. However, quantitative visualization of nanocarriers in the sample’s native environment remains challenging using existing technologies. Single-molecule localization microscopy (SMLM) has the potential to provide both high-resolution visualization and quantitative analysis of nanocarriers in their native environment. However, non-specific binding of fluorescent probes used in SMLM can introduce artifacts, which impose challenges in quantitative analysis of SMLM images. We showed the feasibility of using spectroscopic point accumulation for imaging in nanoscale topography (sPAINT) to visualize self-assembled polymersomes (PS) with molecular specificity. Furthermore, we analyzed the unique spectral signatures of Nile Red (NR) molecules bound to the PS to reject artifacts from non-specific NR bindings. We further developed quantitative spectroscopic analysis for cluster extraction (qSPACE) to increase the localization density by 4-fold compared to sPAINT; thus, reducing variations in PS size measurements to less than 5%. Finally, using qSPACE we quantitatively imaged PS at various concentrations in aqueous solutions with ~20-nm localization precision and 97% reduction in sample misidentification relative to conventional SMLM.
关键词： nanocarriers,Nile Red,super-resolution imaging,single-molecule localization microscopy,spectroscopic analysis,polymersomes
更新于2025-09-23 15:19:57