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[IEEE 2018 IEEE 10th Sensor Array and Multichannel Signal Processing Workshop (SAM) - Sheffield (2018.7.8-2018.7.11)] 2018 IEEE 10th Sensor Array and Multichannel Signal Processing Workshop (SAM) - Subspace-Based Imaging Using Only Power Measurements
摘要： In this paper, we are interested in the high-resolution imaging of an unknown area based on only power measurements of a small number of wireless transceivers located on one side of the unknown area. In order to do so, we propose a framework that achieves a polynomial order reduction in the number of antennas required for high-resolution imaging. More specifically, we show that by spacing the antennas at multiples of the wavelength and applying subspace-based analysis, we can image M targets using only 2M +1 transmit/receive antennas (as compared to the state-of-the-art value of M 2 + 1 antennas). We then validate our framework using simulations in both noise-free and noisy environments.
关键词： Super-resolution imaging,Time Reversal MUSIC,Rytov Model
更新于2025-09-23 15:23:52
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Super-resolution imaging using nano-bells
摘要： In this paper we demonstrate a new scheme for optical super-resolution, inspired, in-part, by PALM and STORM. In this scheme each object in the field of view is tagged with a signal that allows them to be detected separately. By doing this we can identify and locate each object separately with significantly higher resolution than the diffraction limit. We demonstrate this by imaging nanoparticles significantly smaller than the optical resolution limit. In this case the “tag” we have used is the frequency of vibration of nanoscale “bells” made of metallic nanoparticles whose acoustic vibrational frequency is in the multi-GHz range. Since the vibration of the particles can be easily excited and detected and the frequency is directly related to the particle size, we can separate the signals from many particles of sufficiently different sizes even though they are smaller than, and separated by less than, the optical resolution limit. Using this scheme we have been able to localise the nanoparticle position with a precision of ~3 nm. This has many potential advantages - such nanoparticles are easily inserted into cells and well tolerated, the particles do not bleach and can be produced easily with very dispersed sizes. We estimate that 50 or more different particles (or frequency channels) can be accessed in each optical point spread function using the vibrational frequencies of gold nanospheres. However, many more channels may be accessed using more complex structures (such as nanorods) and detection techniques (for instance using polarization or wavelength selective detection) opening up this technique as a generalized method of achieving super-optical resolution imaging.
关键词： optical resolution limit,acoustic vibrational frequency,nanoparticles,nano-bells,super-resolution imaging
更新于2025-09-23 15:21:21
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Specialty probes give super-res imaging that special blink
摘要： Fluorescent probes light the way to cellular detail, but light can also get in the way. Because of the diffraction limit, structures closer to one another than 200 nanometers (nm) or so cannot be discerned. Unless you use probes with super-resolution imaging. These techniques, such as reversible saturable optical linear fluorescence transitions (RESOLFT) or photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM), use specialty probes, dyes and fluorescent proteins (FPs) that can switch from dark to light and from one color to another. 'We need the labels in combination with the microscope to overcome the diffraction barrier,' says Stefan Jakobs, who develops probes at University Medical Center G?ttingen and the Max Planck Institute for Biophysical Chemistry. In structured illumination microscopy (SIM), labs routinely achieve 100-nm resolution, he says. Scientists using stimulated emission depletion microscopy (STED), RESOLFT, PALM or STORM reach beyond 50-nm resolution. In principle, he says, the methods are diffraction unlimited.
关键词： STORM,STED,RESOLFT,super-resolution imaging,SIM,fluorescent probes,diffraction limit,PALM
更新于2025-09-23 15:21:21
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Large field-of-view super-resolution image obtained by manipulating submerged microsphere
摘要： Images with super-resolution can be obtained by dielectric microspheres with appropriate refractive index. However, in practical applications, the microsphere must be moved to the desired position and can scan the large specific area. Here we present a simple method of positioning a microsphere by using a four-dimensional precision translation stage. The simulations have been performed to confirm that 16 μmφ barium titanate glass (BTG) microsphere submerged in alcohol can obtain high quality images and the influence of the probe and the flowing liquid on imaging can be ignored. Therefore, by driving the translation stage, the submerged microsphere can scan the sample surface along the X and Y axis at the speed of 10 μm/s and the large field-of-view image can be achieved by stitching the pictures of different positions. This work has provided a new technique in super-resolution imaging and can be widely applied in field of scientific research.
关键词： photonic nanojet,scanning,super-resolution imaging,large field-of-view,microsphere
更新于2025-09-23 15:21:21
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Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium <em>Prochlorococcus</em>
摘要： Super-resolution microscopy has been widely used to study protein interactions and subcellular structures in many organisms. In photosynthetic organisms, however, the lateral resolution of super-resolution imaging is only ~100 nm. The low resolution is mainly due to the high autofluorescence background of photosynthetic cells caused by high-intensity lasers that are required for super-resolution imaging, such as stochastic optical reconstruction microscopy (STORM). Here, we describe a photobleaching-assisted STORM method which was developed recently for imaging the marine picocyanobacterium Prochlorococcus. After photobleaching, the autofluorescence of Prochlorococcus is effectively reduced so that STORM can be performed with a lateral resolution of ~10 nm. Using this method, we acquire the in vivo three-dimensional (3-D) organization of the FtsZ protein and characterize four different FtsZ ring morphologies during the cell cycle of Prochlorococcus. The method we describe here might be adopted for the super-resolution imaging of other photosynthetic organisms.
关键词： Prochlorococcus,photobleaching,FtsZ ring,Immunology and Infection,STORM,cell division,cyanobacterium,super-resolution imaging,three-dimensional,Issue 141
更新于2025-09-23 15:21:01
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Super-resolution imaging of self-assembled nanocarriers using quantitative spectroscopic analysis for cluster extraction
摘要： Self-assembled nanocarriers have inspired a range of applications for bioimaging, diagnostics, and drug delivery. Non-invasive visualization and characterization of nanocarriers are important for understanding their structure to function relationship. However, quantitative visualization of nanocarriers in the sample’s native environment remains challenging using existing technologies. Single-molecule localization microscopy (SMLM) has the potential to provide both high-resolution visualization and quantitative analysis of nanocarriers in their native environment. However, non-specific binding of fluorescent probes used in SMLM can introduce artifacts, which impose challenges in quantitative analysis of SMLM images. We showed the feasibility of using spectroscopic point accumulation for imaging in nanoscale topography (sPAINT) to visualize self-assembled polymersomes (PS) with molecular specificity. Furthermore, we analyzed the unique spectral signatures of Nile Red (NR) molecules bound to the PS to reject artifacts from non-specific NR bindings. We further developed quantitative spectroscopic analysis for cluster extraction (qSPACE) to increase the localization density by 4-fold compared to sPAINT; thus, reducing variations in PS size measurements to less than 5%. Finally, using qSPACE we quantitatively imaged PS at various concentrations in aqueous solutions with ~20-nm localization precision and 97% reduction in sample misidentification relative to conventional SMLM.
关键词： nanocarriers,Nile Red,super-resolution imaging,single-molecule localization microscopy,spectroscopic analysis,polymersomes
更新于2025-09-23 15:19:57
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0.1THz super-resolution imaging based on 3D printed confocal waveguides
摘要： The paper reports a waveguide-based lens-free terahertz (THz) imaging method. It not only inherits the advantages of traditional confocal imaging, but also realizes super-resolution in THz band. The waveguides prepared by a 3D printing and metal-cladding technology can replace the traditional lens to transmit and focus THz wave effectively. For verification, two hollow waveguides (8 mm inner diameter, 60 mm length) were fabricated and a 0.1 THz confocal waveguides imaging system was built. High quality THz images with a minimum resolution of 1.41 mm (less than 1/2 of the wavelength) were obtained by placing the imaging targets at the waveguide’s focus and performing two-dimensional scanning. The focusing mechanism and transmission characteristics of THz in the waveguide are simulated and analyzed. The simulations are in agreement with the experiments.
关键词： terahertz waveguide,3D printed,confocal system,Super-resolution imaging
更新于2025-09-12 10:27:22
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Faster, sharper, and deeper: structured illumination microscopy for biological imaging
摘要： Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
关键词： Structured illumination microscopy,resolution,biological imaging,super-resolution imaging,imaging speed,depth
更新于2025-09-09 09:28:46
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A 128-Pixel System-on-a-Chip for Real-Time Super-Resolution Terahertz Near-Field Imaging
摘要： This paper presents a fully integrated system-on-a-chip for real-time terahertz super-resolution near-field imaging. The chip consists of 128 sensing pixels with individual cross-bridged double 3-D split-ring resonators arranged in a 3.2 mm long 2 × 64 1-D array. It is implemented in 0.13-μm SiGe bipolar complementary metal–oxide–semiconductor technology and operated at around 550 GHz. All the functions, including sensor illumination, near-field sensing, and detection, are co-integrated with a readout integrated circuit for real-time image acquisition. The pixels exhibit a permittivity-based imaging contrast with a worst case estimated relative permittivity uncertainty of 0.33 and 10–12-μm spatial resolution. The sensor illumination is provided with on-chip oscillators feeding four-way equal power divider networks to enable an effective pixel pitch of 25 μm and a dense fill factor of 48% for the 1-D sensing area. The oscillators are equipped with electronic chopping to avoid 1/f-noise-related desensitization for the SiGe-heterojunction bipolar transistor power detectors integrated at each pixel. The chip features both an analog readout mode and a lock-in-amplifier-based digital readout mode. In the analog readout mode, the measured dynamic range (DR) is 63.8 dB for a 1-ms integration time at an external lock-in amplifier. The digital readout mode achieves a DR of 38.5 dB at 28 f/s. The chip consumes 37–104 mW of power and is packaged into a compact imaging module. This paper further demonstrates real-time acquisition of 2-D terahertz super-resolution images of a nickel mesh with 50-μm feature size, as well as a biometric human fingerprint.
关键词： terahertz,system-on-a-chip (SoC),SiGe heterojunction bipolar transistor (HBT),split-ring resonator (SRR),near-field array,3-push Colpitts oscillator,super-resolution imaging,near-field scanning optical microscopy (NSOM),power detector
更新于2025-09-09 09:28:46