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- 实验方案
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A Speckle-Free Angular Interrogation SPR Imaging Sensor Based on Galvanometer Scan and Laser Excitation
摘要： A speckle-free fast angular interrogation surface plasmon resonance imaging (SPRi) sensor based on a diode-pumped all-solid-state laser and galvanometer is reported in this work. A bidirectional scan using a galvanometer realizes the fast scanning of the incidence angle. The experimental results showed that the time needed for completing an SPR dip measurement was decreased to 0.5 s. And through cascading an immovable diffuser and two diffusers rotating in opposite directions, laser speckle was eliminated. The dynamic detection range and the sensitivity reached 4.6 × 10?2 and 1.52 × 10?6 refractive index unit (RIU), respectively, in a 2D array sensor when the angle scanning range was set as 7.5°. More importantly, the results demonstrated that the angular interrogation SPR imaging sensor scheme had the capability to perform fast and high-throughput detection of biomolecular interactions at 2D sensor arrays.
关键词： Speckle-free,Biomolecule interaction,Angular interrogation,Surface plasmon resonance imaging sensor
更新于2025-11-25 10:30:42
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Rapid label-free detection of intact pathogenic bacteria <i>in situ via</i> surface plasmon resonance imaging enabled by crossed surface relief gratings
摘要： The unique plasmonic energy exchange occurring within metallic crossed surface relief gratings (CSRGs) has recently motivated their use as biosensors. However, CSRG-based biosensing has been limited to spectroscopic techniques, failing to harness their potential for integration with ubiquitous portable electronics. Here, we introduce biosensing via surface plasmon resonance imaging (SPRi) enabled by CSRGs. The SPRi platform is fully integrated including optics and electronics, has bulk sensitivity of 613 Pixel Intensity Unit (PIU)/Refractive Index Unit (RIU), a resolution of 10?6 RIU and a signal-to-noise ratio of ～33 dB. Finite-Difference Time-Domain (FDTD) simulations confirm that CSRG-enabled SPRi is supported by an electric field intensity enhancement of ～30 times, due to plasmon resonance at the metal-dielectric interface. In the context of real-world biosensing applications, we demonstrate the rapid (<35 min) and label-free detection of uropathogenic E. coli (UPEC) in PBS and human urine samples for concentrations ranging from 103 to 109 CFU mL?1. The detection limit of the platform is ～100 CFU mL?1, three orders of magnitude lower than the clinical detection limit for diagnosis of urinary tract infection. This work presents a new avenue for CSRGs as SPRi-based biosensing platforms and their great potential for integration with portable electronics for applications requiring in situ detection.
关键词： crossed surface relief gratings,portable electronics,biosensing,pathogenic bacteria detection,surface plasmon resonance imaging
更新于2025-09-23 15:19:57
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Development of an optoelectronic nose based on surface plasmon resonance imaging with peptide and hairpin DNA for sensing volatile organic compounds
摘要： Nowadays, the analysis of volatile organic compounds (VOCs) is very important in various domains. In the last decades, electronic noses have emerged as promising alternatives to traditional analytical methods. Nevertheless, their wide use is still limited by their performances such as low selectivity. Herein, we developed an optoelectronic nose using virtually screened peptides and hairpin DNA (hpDNA) with improved selectivity as sensing materials and surface plasmon resonance imaging (SPRi) as the detection system. Thanks to the complementarity of their binding properties towards target VOCs, the obtained optoelectronic nose has very good selectivity, being able to discriminate not only between VOCs of different chemical families, but also VOCs of the same family with only 1-carbon difference. The combination of these sensing materials with SPRi is relevant for the development of optoelectronic nose with large sensor arrays and improved performances.
关键词： Volatile organic compounds,Surface plasmon resonance imaging,E-nose,Peptides,Optoelectronic nose,Hairpin DNA
更新于2025-09-19 17:13:59
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[IEEE 2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) - Fukuoka, Japan (2019.5.26-2019.5.29)] 2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) - Odorant-binding protein-based optoelectronic tongue and nose for sensing volatile organic compounds
摘要： We developed an array of odorant-binding protein mutants with various binding properties. The same design is suitable for the detection and identification of volatile organic compounds (VOCs) both in the liquid phase and in the gas phase by surface plasmon resonance imaging. The obtained optoelectronic tongue is highly selective at low concentrations of VOCs with a low detection limit, but a narrow linear range. In comparison, the optoelectronic nose gives a much higher signal to noise ratio, but the discrimination of VOCs from different chemical classes requires kinetic data to get rid of non-specific signals. This work shows that these optoelectronic tongue and nose are promising for numerous applications, each system having its own advantages.
关键词： electronic tongue,surface plasmon resonance imaging,electronic nose,Odorant-binding proteins,volatile organic compound
更新于2025-09-19 17:13:59