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Further Development of Near-Infrared Mediated Quantum Dots and Paclitaxel Co-loaded Nanostructured Lipid Carrier System for Cancer Theragnostic
摘要： Of colloidal systems, ceteris paribus, nanostructured lipid carriers are second to none in offering a single-unit platform for multifunctional benefits. Quantum dots are known to possess unique properties that make them ideal for imaging purpose and that they may be used for cancer detection. For several decades, paclitaxel has been the most effective drug against a wide range of solid tumours. Theragnostic nanomedicine provides a platform to monitor, evaluate, and individualize treatment in real time. Evaluation of cancer treatment outcome at an early stage therapy is key to increase survival prospects of a patient. Previously, a novel co-loaded nanostructured lipid carriers’ theragnostic system for parenteral administration was developed. The aim of this study was to further investigate the co-loaded nanostructured lipid carriers in order to provide interpretation necessary for preclinical elucidation of the formulation, in part. The co-loaded nanostructured lipid carriers were prepared by oil/water emulsification-solvent evaporation technique. In this study, stability and co-loaded nanostructured lipid carriers’ internalization by MCF 7 and HepG2 cells were investigated. The co-loaded nanostructured lipid carriers was stable at 4(cid:2)C for 1 month. The formulation was successfully internalized by MCF-7 and HepG2 cells. Nevertheless, the co-loaded nanostructured lipid carrier was more apt for MCF-7 cells. This finding affirms the formulation to be the most appropriate for breast cancer treatment. In addition, if taken correctly by a patient for a month, the formulation would give true reflection of the contents’ amounts, the factor paramount to appropriate changes in treatment protocol. It can therefore safely be concluded that the co-loaded nanostructured lipid carrier formulation may be potentially an effective theragnostic translational system.
关键词： translational system,cellular uptake,theragnostic,stability study,co-loaded nanostructured lipid carrier
更新于2025-09-23 15:21:01
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Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with <sup>111</sup> In: A Dual Tool for Biodistribution and Stability Studies
摘要： This work aims to provide an effective and novel dual tool for the biodistribution studies of biopolimeric nanoparticles by using modified silk fibroin nanoparticles as a model. This is an indispensable step in the evaluation of the applicability of biopolymeric nanoparticles as drug delivery systems. In this paper, we report a new facile method for radiolabeling silk fibroin nanoparticles conjugated to the chelating agent diethylenetriamine pentaacetic acid (DTPA) and tagged with fluorescein isothiocyanate (FITC). Nanoparticles were characterized by means of dynamic light scattering (DLS), scanning electron microscopy (SEM), infrared and fluorescence spectroscopy. The in vitro studies included the stability in biological media and the evaluation of the cytotoxicity of the nanoparticles in a cell culture. The in vivo study was focused in the scintigraphic study over 24 h conducted on New Zealand rabbits, after intra-articular injection of [111In]In-nanoparticles containing 8.03 ± 0.42 MBq. Biodistribution of the nanoparticles was assessed also ex vivo by fluorescence microscopy of post mortem biopsied organs. This radiolabeling method was reproducible and robust with high radiolabeling efficiency (~80 %) and high specific activity suitable for the in vivo studies. Radiolabeled nanoparticles, having hydrodynamic radius of 113.2 ± 2.3 nm, a polydispersity index (PdI) of 0.101 ± 0.015 and Z-potential of -30.1 ± 2.0 mV, showed and optimum retention in the articular space, without activity clearance up to 24 h post injection. Thus, an easy and robust radiolabeling method has been developed, and its applicability is demonstrated in vitro and in vivo studies, showing its value for future investigation of silk fibroin nanoparticles as versatile and stable (steady) local drug delivery systems for consideration as a therapeutic option, particularly in the treatment of joint disorders.
关键词： biodistribution,theragnostic,radiolabeling,mesenchymal stem cells culture,FITC,silk fibroin nanoparticles,Indium-111,stability
更新于2025-09-23 15:19:57