- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Applications of Molecular Spectroscopy to Current Research in the Chemical and Biological Sciences || Using Fluorescence Spectroscopy to Diagnose Breast Cancer
摘要： Optical spectroscopy methods have had considerable impact in the field of biomedical diagnostics, providing novel methods for the early or noninvasive diagnosis of various medical conditions. Among them, fluorescence spectroscopy has been the most widely explored mainly because fluorescence is highly sensitive to the biochemical makeup of tissues. It has been shown that tumors were easily detected on account of altered fluorescence properties with respect to fluorescence of ordinary tissue. Breast cancer is one of the most commonly diagnosed cancers among women in the world and also it is one of the leading causes of deaths from cancer for the female population. However, when detected in early stage, it is one of the most treatable forms of cancer. Therefore, fluorescence technologies could be highly beneficial in early detection and timely treatment of cancer. This chapter presents main results and conclusions that have been reported on the use of fluorescence spectroscopy for the investigation of breast cancer. It also gives an overview on the instruments and methodology of measurements, on the main endogenous fluorophores present in tissues, on the tissue fluorescence, and on the statistical methods that aid interpretations of fluorescence spectra. Finally, examples of using various fluorescence techniques, such as excitation, emission and synchronous spectroscopy, excitation-emission matrices, and lifetimes, for the breast cancer diagnosis are presented.
关键词： fluorescence,cancer diagnosis,breast cancer,fluorophores,tissue fluorescence
更新于2025-09-10 09:29:36
-
Ultrasound-Switchable Fluorescence Imaging via an EMCCD Camera and a Z-Scan Method
摘要： The spatial resolution of the conventional fluorescence imaging in centimeter-deep tissue is limited due to high light scattering. Ultrasound-switchable fluorescence (USF) imaging was recently developed to achieve high resolution in deep tissue. However, previous frequency-domain USF systems adopted a fiber bundle to collect the emitted fluorescence photons, which led to a few limitations, such as, first, low photon-collection efficiency; second, low efficiency for scanning a sample with an uneven surface; third, low imaging speed, because of the adoption of a raster scan and the long wait time for the tissue to cool down before starting the data acquisition at the next location. In this study, we proposed a camera-based USF imaging system to overcome these limitations. Thanks to the spatial information provided by the camera, a new scan method, Z-scan, was developed, and the imaging speed was improved four times over the raster scan. The USF imaging using the Z-scan for tissue samples was realized. The results were validated by a commercial micro-CT system.
关键词： deep-tissue fluorescence imaging,Ultrasound-switchable fluorescence,biomedical imaging
更新于2025-09-04 15:30:14