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Aminoboronic acid-functionalized graphitic carbon nitride quantum dots for the photoluminescence multi-chemical sensing probe
摘要： This paper reports a highly sensitive photoluminescence glucose sensor based on aminoboronic acid-functionalized carbon nitride quantum dots (g-CNQDs/3APBA) fabricated using melamine and 3-aminophenylboronic acid via a facile two-step synthesis process. By introducing the covalent bonds between g-CNQDs and boronic acid groups, it can be effectively used as “on-off-on” based multi-chemical sensor. The g-CNQDs/3APBA exhibited quantum yields (QYs) as high as 78.5%, which is the highest QYs among fluorescence sensors based on g-CNQDs reported thus far. The material showed a wide linear range of 0 – 10 mM and a detection limit as low as 42 nM with excellent selectivity. In addition, it exhibited comparable performance compared to those of a commercial glucometer in a real blood test. Owing to the excellent bio-imaging properties and low cytotoxicity, g-CNQDs/3APBA is a promising candidate as a sensing material for biomedical and clinical applications.
关键词： quantum dots,glucose sensor,multi-chemical sensor,fluorescence,Graphitic carbon nitride,3-aminophenylboronic acid
更新于2025-11-20 15:33:11
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Fluorometric determination of the activity of alkaline phosphatase and its inhibitors based on ascorbic acid-induced aggregation of carbon dots
摘要： The authors describe a fluorometric method for determination of the activity of alkaline phosphatase (ALP) and its inhibitors. Nitrogen and boron co-doped carbon dots (C-dots) with excitation/emission peaks at 490/540 nm act as the fluorescent probe. The C-dots were prepared by hydrothermal carbonization starting from 3-aminophenylboronic acid as the sole precursor. On the basis of the boronic acid-triggered specific reaction with cis-diols, the boronic acid modified C-dots can bind to ascorbic acid that is generated by ALP-catalyzed hydrolysis of ascorbic acid 2-phosphate. This results in particle aggregation and quenching of fluorescence. If the ALP inhibitor Na3VO4 is introduced into the system, the activity of ALP is reduced and the fluorescence of C-dots recovers. This fluorometric method allows for the determination of ALP activity in the range from 0.2 to 6.0 mU mL?1 with a detection limit of 0.16 mU mL?1. The IC50 value for the inhibitor Na3VO4 is 3.6 μM. The method is convenient and cost-effective. It does not require complicated operations and in our perception widens the scope of applications of C-dots in bioanalytical sciences.
关键词： cis-Diols,3-Aminophenylboronic acid,Enzyme inhibition,Fluorometry,Ascorbic acid,Ascorbic acid 2-phosphate,Sodium orthovanadate
更新于2025-11-19 16:46:39