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Rapid Scanning of the Origin and Antioxidant Potential of Chilean Native Honey Through Infrared Spectroscopy and Chemometrics
摘要： Antioxidant compounds have the ability to trap free radicals; in honey, this capacity is related to the botanical origin of the sample, and therefore, there has been a growing interest in verifying the floral origin of beehive products and its relation with the polyphenolic compounds with potential antioxidant activity. A FTIR spectrum has been use to discriminate floral origin in Chilean monofloral samples and to predict their antioxidant capacity. Forty-nine honey samples from different geographical zones and botanical origin were classified according to melissopalynology analysis, and total phenolic and flavonoid contents were quantified by spectrophotometric methods. Discriminant analysis showed that Quillay (Quillaja saponaria), Corcolén (Azara petiolaris), and Tebo (Retanilla trinervia) honeys showed similarities related to their common geographical origin, while Ulmo (Eucryphia cordifolia) presents a differentiate behavior. The FTIR spectra were able to predict phenolic and flavonoid content, establishing the potential of spectroscopic tools for quality control in Chilean beehive industry.
关键词： PCA,Honeybee,Antioxidant,Melissopalynology,FTIR
更新于2025-11-14 15:18:02
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Suppression of Light-Induced Oxidative Stress in the Retina by Mitochondria-Targeted Antioxidant
摘要： Light-induced oxidation of lipids and proteins provokes retinal injuries and results in progression of degenerative retinal diseases, such as, for instance, iatrogenic photic maculopathies. Having accumulated over years retinal injuries contribute to development of age-related macular degeneration (AMD). Antioxidant treatment is regarded as a promising approach to protecting the retina from light damage and AMD. Here, we examine oxidative processes induced in rabbit retina by excessive light illumination with or without premedication using mitochondria-targeted antioxidant SkQ1 (10-(6’-plastoquinonyl)decyltriphenyl-phosphonium). The retinal extracts obtained from animals euthanized within 1–7 days post exposure were analyzed for H2O2, malondialdehyde (MDA), total antioxidant activity (AOA), and activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) using colorimetric and luminescence assays. Oxidation of visual arrestin was monitored by immunoblotting. The light exposure induced lipid peroxidation and H2O2 accumulation in the retinal cells. Unexpectedly, it prominently upregulated AOA in retinal extracts although SOD and GPx activities were compromised. These alterations were accompanied by accumulation of disulfide dimers of arrestin revealing oxidative stress in the photoreceptors. Premedication of the eyes with SkQ1 accelerated normalization of H2O2 levels and redox-status of lipids and proteins, contemporarily enhancing AOA and, likely, sustaining normal activity of GPx. Thus, SkQ1 protects the retina from light-induced oxidative stress and could be employed to suppress oxidative damage of proteins and lipids contributing to AMD.
关键词： SkQ1,superoxide dismutase,glutathione peroxidase,disulfide dimerization of proteins,visual arrestin,age-related macular degeneration,mitochondria-targeted antioxidant,antioxidant activity,light-induced retinal damage,oxidative stress
更新于2025-09-23 15:23:52
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Antioxidant functionalized silica-coated TiO2 nanorods to enhance the thermal and photo stability of Polypropylene
摘要： Rutile TiO2 is commonly used as UV shielding agent because of the absorption of UV light. In this research, we integrated the function of rutile TiO2 nanoparticles and antioxidants (AO), and prepared a kind of multifunctional TiO2 nanoparticles with the capacity of both UV absorption and antioxidation. Firstly, rutile TiO2 nanorods were prepared and encapsulated by SiO2 to decrease its photocatalytic activity. Then, antioxidant functionalized silica-coated TiO2 nanorods (AO-KH550-SiO2-TiO2) were successfully synthesized by using aminosilane coupling agent (KH550) as a bridge. By melt blending method, AO-KH550-SiO2-TiO2 were incorporated into PP matrix. The results indicated that AO-KH550-SiO2-TiO2 could improve both photo stability and thermal stability of PP. PP/AO-KH550-SiO2-TiO2 film broke after 220 h UV irradiation, and the broken time of PP/TiO2 film was only 80 h. And the oxidation induction time of PP/AO-KH550-SiO2-TiO2 sample was 4.7 min, while PP/TiO2 sample showed no antioxidation effect. In addition, AO-KH550-SiO2-TiO2 exhibited excellent anti-extraction property. This novel design of AO-KH550-SiO2-TiO2 may open up a new avenue for fabricating multifunctional nanoparticles and facilitating their practical application.
关键词： TiO2,antiaging,PP,antioxidant,SiO2
更新于2025-09-23 15:22:29
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Effects of light-emitting diodes on thermally-induced oxidative stress in the bay scallop <i>Argopecten irradians</i>
摘要： Water temperature is an important stressor that affects the physiological and biochemical responses of scallops. In this study, we investigated the effect of different light-emitting diodes (LEDs; red, green and blue) on oxidative stress in Argopecten irradians. PCR revealed MnSOD mRNA expression in the digestive diverticula, gill, adductor muscle and eye. CAT and HSP70 mRNA were expressed in the digestive diverticula, gill and adductor muscle. Additionally, we measured the changes in the expression of HSP70, MnSOD and CAT as well as H2O2 levels during thermal/laboratory stress. In the digestive diverticula, gill and adductor muscle, the mRNA expressions and activities and H2O2 levels significantly increased in response to thermal changes. The gene expressions and activities and H2O2 levels were significantly lower in scallops that received green LED light than in those that received no mitigating treatment. A comet assay revealed that thermal change groups had increased rates of nuclear DNA damage; however, treatment with green LED reduced the frequency of damage. The results indicated that low or high water temperature conditions induced oxidative stress in A. irradians but that green LED significantly reduced this stress.
关键词： short wavelength light,bivalves,Antioxidant,water temperature change
更新于2025-09-23 15:19:57
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Effect of a topical antioxidant serum containing vitamin C, vitamin E, and ferulic acid after Qa??switched 1064a??nm Nd:YAG laser for treatment of environmenta??induced skin pigmentation
摘要： Background: Extrinsic skin aging caused by atmospheric pollutants is associated with a sustained inflammatory response which is a significant risk factor for lentigines and melasma. Aims: The aim of this study was to evaluate the efficacy of topical application of combination formulation of vitamin C, vitamin E, and ferulic acid as an adjuvant to Q-switched Nd:YAG (QSNY) lasers treatment in individuals with lentigines and melasma. Methods: A single blinded, prospective, randomized split-face trial was conducted. Eighteen men and women between 26 and 53 years old were treated with a combination antioxidant serum on one randomized side of their face immediately after QSNY laser and twice daily for 2 weeks. Patients were evaluated using digital photography and spectrometry to assess the melanin index and erythema index. Melasma severity score and global improvement scores also were assessed. Results: The treated side of the face exhibited a significantly greater reduction in the melanin index. There was no significant difference in post-treatment erythema. More clinical improvement was observed on the treated side compared with the untreated side. Conclusions: Our study suggests that topical application of a combination vitamins C, E, and ferulic acid antioxidant formula may be effective as an adjuvant option in QSNY lasers.
关键词： air pollution,lentigo,QS Nd:YAG laser,vitamin C,antioxidant
更新于2025-09-23 15:19:57
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Alleviating the toxicity of quantum dots to Phanerochaete chrysosporium by sodium hydrosulfide and cysteine
摘要： Quantum dots (QDs) have caused large challenges in clinical tests and biomedical applications due to their potential toxicity from nanosize effects and heavy metal components. In this study, the physiological responses of Phanerochaete chrysosporium (P. chrysosporium) to CdSe/ZnS QDs with either an inorganic sulfide NaHS or an organic sulfide cysteine as antidote have been investigated. Scanning electron microscope analysis showed that the hyphal structure and morphology of P. chrysosporium have obviously changed after exposure to 100 nM of COOH CdSe/ZnS 505, NH2 CdSe/ZnS 505, NH2 CdSe/ZnS 565, or NH2 CdSe/ZnS 625. Fourier transform infrared spectroscopy analysis indicated that the existence of hydroxyl, amino, and carboxyl groups on cell surface could possibly conduct the stabilization of QDs in an aqueous medium. However, after NaHS or cysteine treatment, the cell viability of P. chrysosporium exposed to CdSe/ZnS QDs increased as compared to control group, since NaHS and cysteine have assisted P. chrysosporium to alleviate oxidative damage by regulating lipid peroxidation and superoxide production. Meanwhile, NaHS and cysteine have also stimulated P. chrysosporium to produce more antioxidant enzymes (superoxide dismutase and catalase), which played significant roles in the defense system. In addition, NaHS and cysteine were used by P. chrysosporium as sulfide sources to promote the glutathione biosynthesis to relieve CdSe/ZnS QDs-induced oxidative stress. This work revealed that sulfide sources (NaHS and cysteine) exerted a strong positive effect in P. chrysosporium against the toxicity induced by CdSe/ZnS QDs.
关键词： Detoxification,Cysteine,CdSe/ZnS quantum dots,Sodium hydrosulfide,Antioxidant enzymes,Oxidative stress
更新于2025-09-23 15:19:57
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Phytochemical properties and antioxidant activity of Echinops persicus plant extract: green synthesis of carbon quantum dots from the plant extract
摘要： Echinops persicus is a species of Echinops that can be found in Europe and western Asia especially in Iran. In this paper, the phytochemical properties of Echinops persicus were thoroughly investigated and studied. In this way, total amounts of flavonoid, tannins, phenols, carotenoids, saponins, alkaloids, and anthocyanin were measured by various analytical methods. The major phenolic and flavonoid content was found in the root acetone and flower ethanolic extract of the plant. Also, major saponins and carotenoid contents was found in root and leaf of the plant. These measurements were also performed for root, leaf, and flower of the plant in various solvent extracts. The results indicated that Echinops persicus is a rich source of secondary metabolites. Also, the plant extract demonstrated a considerable antiradical activity, which evaluated qualitatively as well as quantitatively. The largest capacity to neutralize DPPH radicals was found for root water extract of Echinops persicus plant. Finally, the possibility of the plant extract was evaluated for the green preparation of carbon quantum dots (CQDs). As-prepared CQDs were characterized by TEM, HRTEM, UV–Vis, XRD, and EDX analyses.
关键词： Antioxidant,Echinops persicus,Secondary metabolites,Green preparation of CQDs,Carbon quantum dots
更新于2025-09-23 15:19:57
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Differential effects of high light duration on growth, nutritional quality, and oxidative stress of hydroponic lettuce under red and blue LED irradiation
摘要： In this study, effects of high light irradiation (HLI, 500 μmol m?2·s?1) duration in the middle of light period (16 h, 150 μmol m?2·s?1) provided by red (R) and blue (B) LEDs (4R:1B) on the growth, nutritional quality, and oxidative stress of hydroponic purple leaf lettuce grown in an environmentally controlled plant factory were examined on the 10th and 20th day after treatment. Set up five treatments with 0 h (CK), 0.5 h (HL0.5), 1 h (HL1), 2 h (HL2), and 4 h (HL4) HLI, respectively. We found that the shoot fresh weight of lettuce increased first and then decreased with the prolongation of HLI duration, whether in the early or late growth stage. On the 10th day after treatment, the root fresh weight and specific leaf weight of lettuce increased linearly with the prolongation of HLI duration, while the shoot/root ratio decreased linearly. On the 20th day after treatment, the lettuce of HL1 had the highest shoot fresh weight, leaf area, and shoot dry weight, followed by HL2. Besides, HL4 promoted the accumulation of soluble sugar, anthocyanin, flavonoid, and total phenolic simultaneously decreased the maximum quantum efficiency compared with others on the 10th day after treatment. On the 20th day after treatment, HL0.5 and HL4 stimulated the accumulation anthocyanin, flavonoid, total phenolic, and ascorbic acid compared with other treatments. The results showed that short-term HLI promotes biomass production and secondary metabolite accumulation, but HL4 caused photo-oxidative damage to lettuce. Therefore, lettuce growth and qualities can be purposely adjusted by adopting different duration of HLI provided by red and blue light in the plant factory, also these responses depended on lettuce growth stage closely.
关键词： Secondary metabolite accumulation,Hydroponic lettuce,High light irradiation,Antioxidant,LED
更新于2025-09-23 15:19:57
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Detection/quantification of amyloid aggregation in solution using the novel fluorescent benzofuranone-derivative compounds as amyloid fluorescent probes: synthesis and in vitro characterization
摘要： Neurodegenerative diseases are characterized by the presence of amyloid deposition. Thioflavin T (ThT) has been one of the molecules of choice to attempt the detection of the amyloid deposits, but ThT is unable to cross blood–brain barrier, due to its low lipophilicity. Therefore, there is strong motivation to design and develop new compounds for in vitro fibril detection as well as for in vivo amyloid imaging. Additionally, the importance and critical role of oxidative stress in the onset/progression of some neurodegenerative disorders, and therefore, the efficacy of aurone compounds in inhibiting the resulting toxicity have been frequently reported. In this study, we report the synthesis of some benzofuranone compounds and examine their antioxidant inhibitory property. Furthermore, to establish the potential detection of synthesized compounds to amyloid aggregates, their in vitro binding to some non-disease related amyloidogenic proteins were characterized. Analyses of the in vitro binding studies showed that compounds 3 and 4 bind to the fibril structures successfully while compounds 1, 2 and 5 indicated a low affinity binding to amyloid. Additionally, compounds 3 and 4 exhibited very good antioxidant properties. Furthermore, these compounds have a great potential as fluorescent probes for detecting amyloid aggregation for further investigations.
关键词： BSA,β-Lactoglobulin,Antioxidant activity,Amyloid determination
更新于2025-09-19 17:15:36
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Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid
摘要： Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay.
关键词： Periodate,C-dots,Antioxidant,Nitrone,Hydrothermal synthesis,Uricase
更新于2025-09-19 17:15:36