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Facile method for iodide ion detection via the fluorescence decrease of dihydrolipoic acid/beta-cyclodextrin protected Ag nanoclusters
摘要： In this work, novel photoluminescent Ag nanoclusters (Ag NCs) with red emission are synthesized and successfully used for detecting iodide ion (I?). The dihydrolipoic acid (DHLA) is used as the stabilizing agent and beta-cyclodextrin (β-CD) is used as the auxiliary stabilizing agent. DHLA and β-CD are combined with Ag atoms by the formation of Ag-S bonds and hydrophobic interaction, respectively. Functionalization of β-CD endows good photoluminescent properties and solubility in water to the Ag NCs. The obtained DHLA and β-CD-protected Ag NCs (DHLA/β-CD-Ag NCs) are spherical and display a dispersed state. However, the DHLA/β-CD-Ag NCs are aggregated in the presence of I?, accompanied by the decrease in their fluorescence intensity. Because the integrity of β-CD cavities is retained on the surface of DHLA/β-CD-Ag NCs, which preserves their capability for I? host–guest recognition, the DHLA/β-CD-Ag NCs combine with I? through the formation of inclusion complexes. Based on this phenomenon, the prepared DHLA/β-CD-Ag NCs can be designed as a novel fluorescent probe for I? detection. The limit of detection (LOD) is calculated as 0.06 μM, indicating that it is an ideal probe for I? detection in practical applications.
关键词： Ag nanoclusters,Fluorescent probe,Beta-cyclodextrin,Dihydrolipoic acid,Iodide ion
更新于2025-09-23 15:23:52
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Pharmacokinetic Properties of Fluorescently Labelled Hydroxypropyl-Beta-Cyclodextrin
摘要： 2-Hydroxypropyl-beta-cyclodextrin (HPBCD) is utilized in the formulation of pharmaceutical products and recently orphan designation was granted for the treatment of Niemann–Pick disease, type C. The exact mechanism of HPBCD action and side effects are not completely explained. We used fluorescently labelled hydroxypropyl-beta-cyclodextrin (FITC-HPBCD) to study its pharmacokinetic parameters in mice and compare with native HPBCD data. We found that FITC-HPBCD has fast distribution and elimination, similar to HPBCD. Interestingly animals could be divided into two groups, where the pharmacokinetic parameters followed or did not follow the two-compartment, first-order kinetic model. Tissue distribution studies revealed, that a significant amount of FITC-HPBCD could be detected in kidneys after 60 min treatment, due to its renal excretion. Ex vivo fluorescent imaging showed that fluorescence could be measured in lung, liver, brain and spleen after 30 min of treatment. To model the interaction and cellular distribution of FITC-HPBCD in the wall of blood vessels, we treated human umbilical vein endothelial cells (HUVECs) with FITC-HPBCD and demonstrated for the first time that this compound could be detected in the cytoplasm in small vesicles after 30 min of treatment. FITC-HPBCD has similar pharmacokinetic to HPBCD and can provide new information to the detailed mechanism of action of HPBCD.
关键词： endocytosis,pharmacokinetics,fluorescence,hydroxypropyl-beta-cyclodextrin,HUVECs
更新于2025-09-16 10:30:52