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[IEEE 2018 Photonics North (PN) - Montreal, QC, Canada (2018.6.5-2018.6.7)] 2018 Photonics North (PN) - Bloch Surface Wave Based Biosensing
摘要： An integrated biosensing platform exploiting the properties of Bloch surface waves on all-dielectric thin film stacks is described. It combines label-free sensing based on tracking with surface wave enhanced angular resonance fluorescence and detection. Detailed design considerations reveal a system with defined attenuation to improve resolution limits in both modes.
关键词： label-free detection,biosensor,Bloch surface wave,1D photonic crystal,surface wave enhanced fluorescence
更新于2025-09-04 15:30:14
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Dual?Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection
摘要： Ratiometric electrochemiluminescence (ECL) has attracted special focus in biological analysis field because it could eliminate the environmental interference to gain precise measurement. Herein, a dual?wavelength ratiometric ECL biosensor was designed for detection of cardiac troponin I (cTnI), where (4,4’?dicarboxylicacid?2,2’?bipyridyl) ruthenium(II) (Ru(dcbpy)3 2+) and Au nanoparticles loaded graphene oxide/polyethyleneimine (GPRu?Au) nanomaterial acts as an acceptor, and Au nanoparticles modified graphitic phase carbon nitride nanosheets composite (Au?CNN) acts as donor. Au?CNN shows high and steady ECL signal centered at 455 nm, which is well matched with the adsorption of GPRu?Au, thereby a high efficient electrochemiluminescent resonance energy transfer (ECL?RET) sensing platform is designed. AuNPs facilitate the immobilization of antibody on the nanomaterials through Au?N bond. The high surface area of graphene oxide/polyethyleneimine allows a large number of Ru(dcbpy)3 2+ to be loaded, which immensely amplify the ECL signal. This sensing platform exhibits outstanding analytical performance toward cTnI with a detection limit of 3.94 fg/mL (S/N=3). The high reliability, selectivity and sensitivity of this ratiometric ECL biosensor provides a versatile sensing platform for the bioanalysis.
关键词： carbon nitride nanosheets,ECL biosensor,graphene oxide,Ratiometric electrochemiluminescence,cardiac troponin I,Au nanoparticles
更新于2025-09-04 15:30:14
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Dual-Channel Photoelectrochemical Ratiometric Aptasensor with Up-converting Nanocrystals Using Spatial-Resolved Technique on Homemade 3D Printed Device
摘要： A near-infrared light (NIRL)-activated ratiometric photoelectrochemical (PEC) aptasensor was fabricated for detection of carcinoembryonic antigen (CEA) coupling with upconversion nanoparticles (UCNPs)-semiconductor nanocrystals-based spatial-resolved technique on a homemade 3D printing device in which a self-regulating integrated electrode was designed for dual signal readout. The as-prepared NaYF4:Yb, Er UCNPs@CdTe nanocrystals were initially assembled on two adjacent photoelectrodes, then CEA aptamer 1 (A1) and capture DNA (CA) were modified onto two working photoelectrodes (WP1 and WP2) through covalent binding, respectively, and then gold nanoparticle-labeled CEA aptamer 2 (Au NP-A2) were immobilized on the surface of functional WP2 for the formation of double-stranded DNA. Upon target CEA introduction, the various concentrations of CEA were captured on the WP1, whereas the binding of the CEA with Au NP-A2 could be released from the WP2 thanks to the highly affinity of CEA toward A2. The dual signal readout with the 'signal-off' of WP1 and 'signal-on' of WP2 were employed for the spatial-resolved PEC (SR-PEC) strategy to detect CEA as an analytical model. Combining NaYF4:Yb, Er UCNPs@CdTe nanocrystals with spatial-resolved model on 3D printing device, the PEC ratiometric aptasensor based on steric hindrance effect and exciton-plasmon interactions (EPI) exhibited a linear range from 10.0 pg mL-1 to 5.0 ng mL-1 with a limit of detection of 4.8 pg mL-1 under 980 nm illumination. The SR-PEC ratiometric strategy showed acceptable stability and reproducibility with a superior anti-interference ability. This approach can provide the guidance for the design of ratiometric, multiplexed and point-of-care biosensors.
关键词： 3D printing device,upconversion nanoparticles,Photoelectrochemical biosensor,ratiometric,spatial-resolved technique,carcinoembryonic antigen
更新于2025-09-04 15:30:14