Determination of cholic acid in body fluids by β?cyclodextrin-modified N-doped carbon dot fluorescent probes

摘要： An easy, dependable, and sensitive cholic acid activity experiment was designed based on β?cyclodextrin-modi?ed carbon dot (β?CD-CD) nanoprobes with speci?c host–guest recognizing ability and photoelectron transfer capability. The β?CD-CD nanoprobes were characterized by infrared, ultraviolet-visible, and ?uorescence spectroscopy and transmission electron microscopy. The ?uorescence of the probes under optimized conditions linearly responded to cholic acid concentration from 0 to 650 μmol·L?1 with a detection limit of 25 nmol·L?1. The probes also performed well in detecting cholic acid in serum and urine samples with an average recovery rate of 97.1%–103.4%. Thus, this study provides a reliable, rapid, and easy method of cholic acid detection in body ?uids that can be potentially applied in medical studies.