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A sequential and reversibility fluorescent pentapeptide probe for Cu(II) ions and hydrogen sulfide detections and its application in two different living cells imaging
摘要： In this study, we report a sequential and reversibility fluorescent probe (DP5) based on pentapeptide conjugated with dansyl groups using the solid phase peptide synthesis (SPPS) technology. DP5 showed immediate “turn off” response toward Cu2+ ions at an excitation wavelength of 330 nm with detection limits of 23.5 nM. The 2:1 binding ratio between DP5 and Cu2+ were confirmed using Job's plot method and fluorescence titration study, and DP5-Cu complex was observed with an association constant of 6.76 × 108 M?2. As designed, DP5-Cu complex as a promising analytical probe exhibited highly selective for H2S detection in aqueous solutions. The detection limit for H2S was obtained to be 17.2 nM, and lower than EPA and WHO guidelines. In addition, the reversibility and cyclicity were imparted to the DP5 during the detection of Cu2+ and H2S, and cycle effect is very good. Furthermore, DP5 displayed better biocompatibility and low biotoxicity, and sequential fluorescence “on-off-on” responses of DP5 to Cu2+ and H2S were successfully applied in two different living cells.
关键词： Cu2+ ions,Pentapeptide,Fluorescent probe,Cell imaging,Hydrogen sulfide,Aqueous solutions
更新于2025-11-21 11:08:12
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Dual-Emitting Quantum Dot Complex Nanoprobe for Ratiometric and Visual Detection of Hg2+ and Cu2+ ions
摘要： Herein we report the use of a dual emitting quantum dot complex (QDC; composed of blue emitting metal-methylsalicylaldimine complex being on the surface of orange emitting Mn2+-doped ZnS quantum dot) for ratiometric and visual detection of Hg2+ and Cu2+ ions - following the alterations in luminescence color, intensity ratio, chromaticity and hue of the QDC.
关键词： Hg2+ ions,Cu2+ ions,ratiometric detection,quantum dot complex,visual detection
更新于2025-09-23 15:21:01
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AIE based “on-off” fluorescence probe for the detection of Cu2+ ions in aqueous media
摘要： A simple hydrazine carboxamide derivative with intramolecular charge transfer and aggregartion induced emission (AIE) properties have been designed and synthesized. The derivative (Probe 1) shows typical AIE characteristic emission in THF-water mixtures and emits yellowish orange color on reaching the water fraction 98%. The probe molecule show selective sensing response for Cu2+ ions via fluorescence turn-off mechanism in aqueous media over other metal ions. The emission changes towards Cu2+ ions could be clearly observed by the naked eyes under 365 nm UV lamp promote the probe molecule as a promising candidate for practical utilization. Furthermore, from the fluorescence titration data, the detection limit for Cu2+ ions was found to be 0.44 μM.
关键词： AIE,visual sensing,Cu2+ ions
更新于2025-09-23 15:21:01