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Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules
摘要： Thermal plasmas and lasers are used in medicine to cut and ablate tissues and for coagulation. Non-equilibrium atmospheric pressure plasma (NEAPP) is a recently developed, non-thermal technique with possible biomedical applications. Although NEAPP reportedly generates reactive oxygen/nitrogen species, electrons, positive ions, and ultraviolet radiation, little research has been done into the use of this technique for conventional free radical biology. Recently, we developed a NEAPP device with high electron density. Electron spin resonance spin-trapping revealed ?OH as a major product. To obtain evidence of NEAPP-induced oxidative modifications in biomolecules and standardize them, we evaluated lipid peroxidation and DNA modifications in various in vitro and ex vivo experiments. Conjugated dienes increased after exposure to linoleic and α-linolenic acids. An increase in 2-thiobarbituric acid-reactive substances was also observed after exposure to phosphatidylcholine, liposomes or liver homogenate. Direct exposure to rat liver in saline produced immunohistochemical evidence of 4-hydroxy-2-nonenal- and acrolein-modified proteins. Exposure to plasmid DNA induced dose-dependent single/double strand breaks and increased the amounts of 8-hydroxy-2'-deoxyguanosine and cyclobutane pyrimidine dimers. These results indicate that oxidative biomolecular damage by NEAPP is dose-dependent and thus can be controlled in a site-specific manner. Simultaneous oxidative and UV-specific DNA damage may be useful in cancer treatment.
关键词： 8-OHdG,HNE-modified protein,non-equilibrium atmospheric pressure plasma,electron spin resonance spin-trapping,UV
更新于2025-09-23 15:22:29
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Formation of lipid bilayers on the pore walls of macroporous silicon
摘要： Electron paramagnetic resonance (EPR) spectroscopy was used to study the pore filling of macroporous silicon with lipid vesicles (liposomes), added with a spin label. Different EPR spectra were obtained with the magnetic field parallel and perpendicular to the macroporous silicon sample surface. These spectra could be well simulated with an admixture of the isotropic spectrum of liposomes, plus a simulated spectrum corresponding to a cylindrical distribution of lipid bilayers. This means that a portion of the liposomes were disrupted, and supported lipid bilayers were formed covering the inner surface of the pores. Diverse protocols can be explored in order to optimize the lipid covering of the pore walls, and to achieve an adequate lipid hydration. This system can be used as a platform to study lipid phase transitions in a confined environment, and to characterize membrane proteins.
关键词： Liposomes,Electron paramagnetic resonance/electron spin resonance,Supported lipid bilayers,Porous silicon,Spin labels
更新于2025-09-19 17:15:36
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Analysis of the charge transfer and separation in electrically doped organic semiconductors by electron spin resonance spectroscopy
摘要： We investigated the charge generation mechanism of electrically doped organic semiconductors (OSs) by electron spin resonance (ESR) analysis. ESR spectroscopy was used to successfully evaluate the radical density of p-doped OSs to estimate the charge transfer efficiency (CTE) of various doped systems. The results showed that the CTE is efficient close to 100% if the dopant molecules are homogenously dispersed and the energy difference (?E) between the highest occupied molecular orbital (HOMO) level of the host molecule and lowest unoccupied molecular orbital (LUMO) level of the p-dopant is large. The charge separation efficiency to form free carriers from the radicals is rather low (less than 12% in this study) and is a dominant factor controlling the charge generation efficiency (CGE). An organic dopant molybdenum tris[1,2-bis(trifluoromethyl)ethane-1,2-dithiolene] turns out to be an efficient dopant with the CGE of 9.7% due to high CTE originating from homogenous dispersion of the organic p-dopants and low LUMO level, i.e., large ?E.
关键词： charge generation efficiency,p-dopant,charge transfer,electron spin resonance spectroscopy,Organic semiconductors,charge separation
更新于2025-09-19 17:15:36
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Oxygen vacancy induced ferromagnetism in ball milled Zn0.97Ni0.03O: Confirmation through electron spin resonance
摘要： Among the semiconducting materials, ZnO is a versatile multifunctional candidate with a direct band gap of 3.37 eV at 300 K and large excitonic binding energy of 60 meV which can be useful for spintronics device applications. ZnO has high solubility for transition metals. The transition metals which have been used for DMS, Ni is the most efficient doping element to improve the electrical, magnetic properties of ZnO. Ni doped ZnO would be a good candidate to achieve ferromagnetic property with a high curie temperature i.e. above the room temperature. In this study, Zn1?xNixO (x = 0.03) powder sample was successfully synthesized by a ball milling technique. The X-ray diffraction analysis confirms the polycrystalline, hexagonal wurzite structure for 3% Ni doped ZnO nanoparticles. The substitution of Ni in the ZnO matrix has been confirmed by micro-Raman analysis with the observation of E2 (High) vibrational mode at 437 cm?1 which refers to the strongest mode of vibration in wurtzite crystal structure. A broadened peak observed at 570 cm?1 informs about the presence of clusters of oxygen vacancies. VSM measurement of the sample shows the ferromagnetic hysteresis loop at room temperature with saturation moment of 9 × 10?5 emu/g. The calculated ‘g’ value of 1.95 from electron spin resonance spectrum suggests that the observed ferromagnetism is due to the oxygen vacancy.
关键词： Diluted magnetic semiconductor,Electron spin resonance,Defects,Ferromagnetism,ZnO,Nanoparticles
更新于2025-09-19 17:13:59
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Anti-inflammatory effect of photodynamic therapy using guaiazulene and red lasers on peripheral blood mononuclear cells
摘要： To evaluate the anti-inflammatory effect of photodynamic therapy using guaiazulene and a red laser in peripheral blood mononuclear cells (PBMCs). Guaiazulene solutions (1, 2, 5, 25, 35, and 100 μM in 99.8% methanol) were irradiated with red laser light (625 nm, 151.5 mW/cm2) in continuous mode at 0, 4, and 8 J/cm2 in black 96-well plates. ROS were measured using spin trapping technique with electron spin resonance (ESR) spectroscopy and fluorescence. The two highest concentrations were tested using cell viability (PrestoBlue?) and anti-inflammation (RANTES and PGE2 ELISA) assay kits. Kruskal-Wallis and Dunn Bonferroni tests were used for statistical analyses with significant differences at p-value < 0.05. Guaiazulene solutions between 2 and 5 μM exposed to red laser light at 4-8 J/cm2 generated significantly more singlet oxygen compared to the no guaiazulene group (p < 0.01) and reduced RANTES and PGE2 levels in TNF-α-inflamed peripheral blood mononuclear cells without affecting cell viability. Photodynamic activation of guaiazulene generated singlet oxygen and suppressed inflammatory markers in PBMCs.
关键词： Electron spin resonance spectrometer,RANTES,Fluorescence probe,Singlet oxygen,PGE2,Guaiazulene
更新于2025-09-19 17:13:59
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[IEEE 2018 Conference on Optoelectronic and Microelectronic Materials and Devices (COMMAD) - Perth, Australia (2018.12.9-2018.12.13)] 2018 Conference on Optoelectronic and Microelectronic Materials and Devices (COMMAD) - Isotopically Pure Silcon-28 Whispering Gallery Mode Resonators: A Host for Narrow Linewidth Spin Ensembles
摘要： Single crystal isotopically pure 28Si cylindrical Whispering Gallery (WG) mode resonators have been machined from a rod of isotopically pure crystal. Before machining, the rod was loaded into a cavity with the best Bragg confined modes exhibiting Q-factors above a million for frequencies between 10 and 15 GHz. Electron Spin Resonance spectroscopy revealed a very narrow linewidth spin transition, with g-factor of 1.995±0.008. Analysis determined an upper limit to the linewidth of 7 kHz and a concentration of less than 1011 spins/cm3 (10 parts per trillion). After machining into WG mode resonators, the measured frequencies of the fundamental mode families were used to determine the relative permittivity of the material near 4 K and 20 mK to be 11.488±0.024, with the precision limited only by the dimensional accuracy of the resonator. However, the Q-factors were degraded to below 40,000. Raman spectroscopy revealed strain induced broadening on the radial surface of the crystal as a result of the machining. After an acid clean and etch, followed by annealing, the surface damage was repaired. Subsequently, high Q-factors were also restored. The next step will be to purposefully implant ions to try and realise narrow linewidth spin ensembles with clock transitions, which will couple to high-Q WG modes inside the crystal.
关键词： Narrow linewidth spin ensembles,Isotopically pure silicon-28,Q-factors,Electron Spin Resonance spectroscopy,Whispering Gallery Mode Resonators
更新于2025-09-11 14:15:04
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Point defect chemistry of donor-doped bismuth titanate ceramic
摘要： This communication reports on the defect chemistry at room temperature of barium (Ba) doped bismuth titanate (Bi4Ti3O12), emphasizing the influence of the point defects on its electrical properties. Pure and Ba doped Bi4Ti3O12 were prepared by a conventional solid-phase reaction technique. The addition of Ba into the crystal structure of Bi4Ti3O12 was monitored by X-ray diffraction measurements combined with Rietveld refinement studies where it was determined that Ba occupies the bismuth (Bi) lattice sites as well as the presence of oxygen vacancies (V??O). The characterization of the point defects was carried out using impedance and electron spin resonance spectroscopies where the results support the models of compensation mechanisms dominated by electrical positive charges (h?) and oxygen vacancies (V??O).
关键词： defect chemistry,X-ray diffraction,Rietveld refinement,impedance spectroscopy,solid-phase reaction,barium doped bismuth titanate,electron spin resonance spectroscopy,electrical properties,oxygen vacancies
更新于2025-09-04 15:30:14