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Cladding of Stellite-6/WC Composites Coatings by Laser Metal Deposition
摘要： This study aims to investigate the microstructure and hardness of multi-layered Stellite-6/WC metal-matrix composites coatings on metallic substrates cladded by laser metal deposition (LMD) for improvement of wear and corrosion resistances. As coating materials, Stellite-6 and WC-12wt.%Co powders were selected. Powder mixtures having various mixing-ratios of Stellite-6 and WC-12wt.%Co were provided vertically on S45C substrates by controlling powder feeding rates of the two powder feeders, individually. Stellite-6/WC composites which consist of three layers with different compositions were cladded on the S45C substrates by laser melting. Cross-sectional microstructure observation was carried out by using an optical microscope (OM). Vickers microhardness tests were conducted to evaluate hardness of the cladding layers and substrates. The experimental results demonstrate that hard multi-layered Stellite-6/WC metal-matrix composites coatings were successfully cladded on the S45C substrates. Property gradients in the Stellite-6/WC composites could be made due to the position-dependent chemical composition and microstructure made by controlling powder feeding rates of an LMD system.
关键词： Cladding,Additive Manufacturing (AM),Metal Matrix Composites (MMC),Laser Metal Deposition (LMD),Hard Materials
更新于2025-09-23 15:22:29
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Analysis of helium used as protective gas in Laser Metal Deposition of Ti6Al4V highly reactive material
摘要： Laser Material Deposition (LMD) is an additive manufacturing near-net-shape process. Metal powder particles are molten by means of a laser heat source and added to a substrate creating geometries. This technology offers the possibility of generating coatings, repairing damaged high-added-value parts, and manufacturing components with complex 3D geometries. Many industrial sectors, including aerospace and die and mold industries, are interested in this process due to its capabilities and the wide range of materials that can be used with it. Previous works aimed to develop a protective gas module to be able to process highly reactive materials without the necessity of a complete inert atmosphere inside the machine. Additionally, the influence of a mixture of argon and helium on the LMD process for other non-highly reactive alloys was evaluated. Hence, the present work aims to broaden the understanding of how the combination of these two factors may affect the LMD process, combining these previous studies and evaluating the phenomenon for a highly reactive alloy like Ti6Al4V. The study involves the melt-pool temperature measurement and characterization of the deposited clads variating not only the composition of the protective gas but also the flow rates.
关键词： LMD,Ti6Al4V,Helium,Titanium,Shielding gas,protective atmosphere,He,Laser Metal Deposition,Argon,Ar
更新于2025-09-23 15:21:01
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Surface based variable thickness slicing modeling for laser metal deposition
摘要： Laser metal deposition (LMD) has been a promising additive manufacturing technology widely used in mold rapid manufacturing. In order to improve the capacity of LMD for complex curved surface structures, a surface based variable thickness slicing (S-VTS) model is proposed to adaptively generate the LMD process based on the geometric characteristics of structures. Two deposition strategies in scanning and overlapping directions are designed to enable variable thickness of each cladding layer by dynamically adjusting the scanning speed and overlapping rate. To improve the surface quality and forming efficiency, the discrete particle swarm optimization (DPSO) algorithm is adopt to optimize the process parameters of S-VTS model, including the number of cladding layer, scanning pass, and segment per pass. Several experiments are conducted to form the cuboid samples with wavy and freeform surface and verify the feasibility of the S-VTS model. The results demonstrate that under the open-loop control condition, the geometric accuracy, surface quality, and efficiency of the proposed method is improved in comparison with uniform thickness slicing (UTS) deposition. Moreover, heterogeneous microstructure is always generated by the S-VTS method in terms of grain size and growth direction.
关键词： Variable thickness slicing (S-VTS),Laser metal deposition (LMD),Curved surface,Mold
更新于2025-09-23 15:19:57
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Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
摘要： Neglecting tissue heterogeneity during the analysis of microRNA (miRNA) levels results in average signals from an unknown mixture of different cell types that are difficult to interpret. Here we demonstrate the technical requirements needed to obtain high-quality, quantitative miRNA expression information from tumor tissue compartments obtained by laser microdissection (LMD). Furthermore, we show the significance of disentangling tumor tissue heterogeneity by applying the newly developed protocols for combining LMD of tumor tissue compartments with RT-qPCR analysis to reveal compartment-specific miRNA expression signatures. An important advantage of this strategy is that the miRNA signature can be directly linked to histopathology. In summary, combining LMD and RT-qPCR is a powerful approach for spatial miRNA expression analysis in complex tissues, enabling discovery of disease mechanisms, biomarkers and drug candidates.
关键词： laser microdissection (LMD),biomarker discovery,tumor microenvironment,drug discovery,microRNA profiling,tissue complexity
更新于2025-09-19 17:13:59
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Laser metal deposition of copper on diverse metals using green laser sources
摘要： Green laser sources are advantageous in the processing of copper due to the increase of absorptivity compared with more commonly available infrared lasers. Laser metal deposition of copper with a green laser onto various substrate metals namely copper, aluminium, steel and titanium alloy was carried out and observed through high-speed imaging. The effects of process parameters such as laser power, cladding speed and powder feed rate, and material attributes such as absorptivity, surface conditions and thermal conductivity are tied together to explain the size and geometry of the melt pool as well as the fraction of the power used for melting material. The copper substrate has the smallest melt pool with a high angle, followed by aluminium, steel and titanium alloy. The incorporation times for powder grains in the melt pools vary based on the substrate materials. Its dependency on material properties, including surface tension forces, melting temperatures and material density, is discussed. Oxide skins present on melt pools can affect powder incorporation, most significantly on the aluminium substrate. The lower limits of the fraction of power irradiated on the surface used purely for melting were calculated to be 0.73%, 2.94%, 5.95% and 9.78% for the copper, aluminium, steel and titanium alloy substrates, respectively, showing a strong dependence on thermal conductivity of the substrate material. For a copper wall built, the fraction was 2.66%, much higher than a single clad on a copper substrate, due to reduced workpiece heating. The results of this paper can be transferred to other metals with low absorptivity such as gold.
关键词： Multi-material,DED,High-speed imaging,LMD,Copper,Green 515-nm laser,Laser metal deposition,Powder grain incorporation,Additive manufacturing,Directed energy deposition,Absorptivity
更新于2025-09-19 17:13:59
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Application of Laser Metal Deposition for a New Model of Assembled Camshaft
摘要： This work introduces the invention of an alternative camshaft manufacturing method aimed at improving the efficiency of the process and providing a more flexible product. The innovation consists in the laser metal deposition (LMD) of a profile on the shaft in order to realize a coupling stretch where the cam is fixed by interference. Alternative profile materials (1.4404 steel and Inconel 625 alloy) are deposited on a 1.0421 steel tube at different laser power levels and powder flow rate. The effect of the process parameters on the deposited profile is investigated initially through the evaluation of indirect quality attributes, such as deposit dimensions, hardness values, and microstructure. Then, the coupling force resulting from the press-fit test is evaluated. Finally, the best configuration of this new assembled camshaft is tested with a torsion test rig. Performances of the new assembled camshaft are compared with those of traditional products, leading to the validation of this innovative solution. The preliminary feasibility of the LMD application to the production of an assembled camshaft is demonstrated.
关键词： additive manufacturing,cladding,hardness,testing,laser metal deposition (LMD),dilution
更新于2025-09-16 10:30:52
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[Laser Institute of America ICALEO? 2016: 35th International Congress on Applications of Lasers & Electro-Optics - San Diego, California, USA (October 16–20, 2016)] International Congress on Applications of Lasers & Electro-Optics - LMD process diagrams for blade tip repair
摘要： Laser Metal Deposition (LMD) is a technology often used for local repairs of e.g. high quality and high value its specific turbomachinery components. Due advantages such as low heat input into the work piece, low distortion, high reproducibility and the suitability for automation, LMD is especially used for blade tip-repair. In general, existing repair processes and results cannot be transferred to new repair applications without substantial adaptations. The development of new tip repair processes strongly depends on the part geometry and the specific materials. For the development of a new tip repair application, the LMD process parameters – such as laser power, scanning speed, powder mass flow as well as beam diameter – have to be adapted and require intensive experimental work. Although this approach is still state of the art, it is time consuming and cost intensive. LMD process diagrams can significantly reduce those experimental efforts and shorten the development time for new repair applications.
关键词： process diagrams,blade tip repair,Laser Metal Deposition,turbomachinery components,LMD
更新于2025-09-16 10:30:52
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In-process height control during laser metal deposition based on structured light 3D scanning
摘要： Laser metal deposition is an additive manufacturing process capable of layer by layer construction of solid parts. An incorrect deposition distance between nozzle and substrate can result in height deviations from the ideal building path. The error can be propagated along layers, leading to an irreversible defect. Maintaining an optimal distance between the nozzle and the substrate is therefore a critical aspect. This paper introduces an off-line geometric control system for in-process measurement and build path correction. A structured light based 3D scanner measures the build height from an external, fixed position, providing more precise results than moving measuring systems. The deposition is stopped in certain stages of the process and the piece is scanned generating a three-dimensional point cloud. Processing of the collected data allows the build height determination. A control algorithm was developed to take corrective actions in accordance with the measured error. The system enables an accurate control of the generated geometry and permits the comparison with the original computer design. This approach reduces the required human supervision during the process and the occurrence of non-valid parts. The effectiveness of the method was proved through the contrast of parts built with and without control, showing a more accurate reproduction of the generated solid parts when corrections are applied.
关键词： monitoring,Laser Metal Deposition (LMD),additive manufacturing,control,metrology,structured light,3D scanning
更新于2025-09-12 10:27:22
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Numerical approach for the estimation of the deposited material on the laser material deposition process
摘要： The Laser Material Deposition (LMD) is an additive manufacturing technique that has become one of the most noteworthy methods for coating and repairing components. It is also a key process in hybrid manufacturing systems that combine LMD and machining operations. One of the main difficulties of LMD is the large number of relevant parameters and the complex interdependencies that must be considered. Thus, process fitting is usually based on trial and error techniques. The present work proposes a series of developments based on the numerical modeling of the LMD process, with the aim of reducing the experimental tests to setup the process.
关键词： Additive Manufacturing,CFD,Laser Material Deposition,LMD,Numeric Model,Powder
更新于2025-09-12 10:27:22
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A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
摘要： Background: Plant development is controlled by the action of many, often connected gene regulatory networks. Differential gene expression controlled by internal and external cues is a major driver of growth and time specific differentiation in plants. Transcriptome analysis is the state-of-the-art method to detect spatio-temporal changes in gene expression during development. Monitoring changes in gene expression at early stages or in small plant organs and tissues requires an accurate technique of tissue isolation, which subsequently results in RNA of sufficient quality and quantity. Laser-microdissection enables such accurate dissection and collection of desired tissue from sectioned material at a microscopic level for RNA extraction and subsequent downstream analyses, such as transcriptome, proteome, genome or miRNA. Results: A protocol for laser-microdissection, RNA extraction and RNA-seq was optimized and verified for three distant angiosperm species: Arabidopsis thaliana (Brassicaceae), Oryza sativa (Poaceae) and Eschscholzia californica (Papaveraceae). Previously published protocols were improved in processing speed by reducing the vacuum intensity and incubation time during tissue fixation and incubation time and cryoprotection and by applying adhesive tape. The sample preparation and sectioning of complex and heterogenous flowers produced adequate histological quality and subsequent RNA extraction from micro-dissected gynoecia reliably generated samples of sufficient quality and quantity on all species for RNA-seq. Expression analysis of growth stage specific A. thaliana and O. sativa transcriptomes showed distinct patterns of expression of chromatin remodelers on different time points of gynoecium morphogenesis from the initiation of development to post-meiotic stages. Conclusion: Here we describe a protocol for plant tissue preparation, cryoprotection, cryo-sectioning, laser microdissection and RNA sample preparation for Illumina sequencing of complex plant organs from three phyletically distant plant species. We are confident that this approach is widely applicable to other plant species to enable transcriptome analysis with high spatial resolution in non-model plant species. The protocol is rapid, produces high quality sections of complex organs and results in RNA of adequate quality well suited for RNA-seq approaches. We provide detailed description of each stage of sample preparation with the quality and quantity measurements as well as an analysis of generated transcriptomes.
关键词： Non-model species,Cryosectioning,Evo-devo,Development,RNA-seq,Laser microdissection (LMD)
更新于2025-09-12 10:27:22