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[IEEE 2018 IEEE Life Sciences Conference (LSC) - Montreal, QC, Canada (2018.10.28-2018.10.30)] 2018 IEEE Life Sciences Conference (LSC) - Assay Development and Storage for Fluorescence-Based Lateral Flow Immunoassay
摘要： Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.
关键词： storage,immunoassay,sensitivity,Lateral flow assay,fluorescence,point-of-care
更新于2025-09-23 15:22:29
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Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods
摘要： The presented data refer to optimization and quantitative characterization of a rapid lateral flow assay based on high-affinity bifunctional ligand and magnetic nanolabels, which was developed for detection of small molecules of thyroid hormones. The results were obtained by including the magnetic particle quantification method, spectral-correlation interferometry and spectral-phase interferometry, dynamic light scattering, enzyme linked immunosorbent assay. The long-term stability of "antibody – magnetic nanoparticle" conjugates is shown. The assay specificity is confirmed, and verification of successful combination of magnetic particles and antibodies is demonstrated. The kinetic and equilibrium dissociation constants are determined for interactions between thyroxine and monoclonal antibodies. The obtained data could be used for design of other platforms for detection of small molecules.
关键词： ELISA,Lateral flow assay,Small molecules,Ultrasensitive detection,Free thyroxine,Interferometry,Magnetic nanolabels,Dynamic light scattering
更新于2025-09-23 15:22:29
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Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
摘要： A novel lateral flow assay (LFA) was developed by introducing Eu (III)-doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05–10 ng/mL, respectively. The recovery of LFA for the detection of SM2 in raw milk was 96.1–108.2%. The proposed LFA provides a rapid and convenient strategy for fast and ultra-sensitive screening of SM2 in raw milk. EuNP-LFA may be a remarkable method for the detection of other targets at low concentrations to ensure food safety.
关键词： Lateral flow assay,sulfamethazine,raw milk,Eu (III)-doped polystyrene nanoparticle
更新于2025-09-23 15:21:21
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Signal amplification and quantification on lateral flow assays by laser excitation of plasmonic nanomaterials
摘要： Lateral flow assay (LFA) has become one of the most widely used point-of-care diagnostic methods due to its simplicity and low cost. While easy to use, LFA suffers from its low sensitivity and poor quantification, which largely limits its applications for early disease diagnosis and requires further testing to eliminate false-negative results. Over the past decade, signal enhancement strategies that took advantage of the laser excitation of plasmonic nanomaterials have pushed down the detection limit and enabled quantification of analytes. Significantly, these methods amplify the signal based on the current LFA design without modification. This review highlights these strategies of signal enhancement for LFA including surface enhanced Raman scattering (SERS), photothermal and photoacoustic methods. Perspectives on the rational design of the reader systems are provided. Future translation of the research toward clinical applications is also discussed.
关键词： gold nanoparticles,lateral flow assay,nanoparticle heating,signal amplification and quantification,SERS
更新于2025-09-23 15:19:57
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Feasibility of a point-of-care test based on quantum dots with a mobile phone reader for detection of antibody responses
摘要： We developed a novel and portable fluorescent sensor that integrates a lateral flow assay with a quantum dot (Qdots) label and a mobile phone reader for detection of specific antibodies in human serum. We evaluated the utility of this assay to test for antibodies to the Taenia solium rT24H antigen. It was a retrospective study by examining 112 positive human sera from patients with neurocysticercosis (NCC) including samples from patients with single viable cyst (n = 18), two or more viable cysts (n = 71), and subarachnoid (racemose) cysts (n = 23). These samples were collected from previous study subjects in Lima, Peru that conducted under an approved study protocol in Peru. The sera were made anonymous under a protocol approved by the CDC Institutional Review Board. Definitive diagnosis of the specimen was established by computed-tomography and/or magnetic resonance imaging. To test the specificity of the assay, we evaluated a panel of serum samples obtained from patients with other infections (n = 24), and serum samples from persons in the United States and Egypt who had not traveled outside their country, and therefore are presumed negative for cysticercosis (n = 128). The assay specificity in the negative panel was 99% (95–100%) while assay sensitivity was 89% (79–95%) in NCC patients with two or more viable cysts. Our assay has performance characteristics similar to those of traditional platforms for the detection of NCC and shows promise as a mobile phone reader-based point-of-care test for antibody detection.
关键词： quantum dots,point-of-care test,mobile phone reader,lateral flow assay,neurocysticercosis
更新于2025-09-16 10:30:52
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[IEEE 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Honolulu, HI, USA (2018.7.18-2018.7.21)] 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Point-of-Care Assessment of Folate Status in Women of Reproductive Age Using a Fluorescence Lateral Flow Assay <sup>*</sup>
摘要： Folate is an essential vitamin to the development of a fetus in early pregnancy. Maternal folate supplementation around the time of conception has been shown to decrease the risk of neural tube defects (NTDs), a class of serious birth defects. The closure of the neural tube before the 28th day after conception necessitates that the folate intake must take place before most women know that they are pregnant. Therefore, screening women of reproductive age for folate status would allow for an improved understanding of the need for supplementation in women who could become pregnant as well as the effectiveness of current supplementation and fortification recommendations. Current folate assessment is limited to lab-based assays which require expensive equipment, trained personnel, and are time-intensive. Our point-of-care diagnostic test quantifies levels of folate in human serum with the use of a lateral flow assay and a portable imaging device. We have designed an assay which uses fluorescent particles, folate binding protein, and antibodies to measure serum folate. This test could be used in resource-limited settings, where access to laboratory infrastructure is limited and where knowledge of folate status in women of reproductive age is lacking. By increasing our understanding of folate status around the world, we can improve implementation of folic acid supplementation and fortification and therefore reduce the risk of NTDs.
关键词： portable imaging device,neural tube defects,point-of-care diagnostic,Folate,lateral flow assay
更新于2025-09-10 09:29:36
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Development of up-converting phosphor technology-based lateral flow assay for quantitative detection of serum PIVKA-II: Inception of a near-patient PIVKA-II detection tool
摘要： Background: Development of a test card based on up-conversion phosphor technology-based immune lateral flow (UPT-LF) assay as a near-patient detection tool for serum Prothrombin induced by vitamin K absence or antagonist II (PIVKA-II). Methods: Up-converted phosphor nanoparticles (UCPs) were used to bind to PIVKA-II monoclonal antibodies as labeled probes to develop the test card for detecting serum PIVKA-II. The UPT-LF test card was evaluated by the limit of detection, linearity, stability, recovery rate, precision and interference. Preliminary clinical validation was conducted by detection of 498 serum samples from 228 patients with hepatocellular carcinoma (HCC), 170 patients with liver benign lesion (LBL) and 100 healthy controls (HC). Additionally, the correlation of serum PIVKA-II detection between UPT-LF assay and Chemiluminescence enzyme immunoassay (CLEIA) assay were performed. Results: Modified and activated UCPs bound to monoclonal antibodies powerfully to form the luminescent labeled probes. Limit of detection and linear range of UPT-LF test card for serum PIVKA-II were 2.66 and 4.8–20,000 ng/ml, respectively. Test card had good 25 °C thermal and 4 °C validity period stability, 93.1%–99.2% of recovery rate, 2.6–5.8% and 5.4–8.9% of intra-assay and inter-assay CVs, and strong anti-interference ability for 8 common serum analytes. The sensitivity and specificity (vs LBL + HC group) of test card for HCC were 71.5% and 88.9%, respectively. The R2 between UPT-LF assay and CLEIA assay was 0.901. Conclusions: UPT-LF assay provides a reliable, rapid and convenient test for quantitative detection of serum PIVKA-II as well as diagnosis of HCC by a point of care testing way.
关键词： Point of care testing,Hepatocellular carcinoma,PIVKA-II,Lateral flow assay,Up-conversion phosphor technology
更新于2025-09-10 09:29:36
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A novel SERS-based lateral flow assay for differential diagnosis of wild-type pseudorabies virus and gE-deleted vaccine
摘要： Pseudorabies virus (PRV) is a pathogen that causes an acute infectious disease in pigs, which could lead to huge losses to the farming industry. The Bartha-K61 strain of PRV, commonly used as a gE-deleted vaccine, does not always protect against the wild-type virus infection effectively. Therefore, the prompt detection of viral infection in gE-deleted vaccine vaccinated pigs is crucial for in-time measures to prevent the spread of diseases. In this study, we developed a Surface-enhanced Raman spectroscopy(SERS) based lateral flow assay based on antigen-antibody reaction to meet the demand. Our method was rapid (15 min), sensitive (LOD: 5 ng mL?1), selective for wild-type PRV detection, and quantitatively or semi-quantitatively (DLR: 41-650 ng mL-1) compatible. The detection results from this method were consistent with results from the gE-specific PCR, indicating that this SERS-based lateral flow assay could be used as a new tool to differentially diagnose wild-type PRV and gE-deleted vaccine.
关键词： differential diagnosis,AuAg4-ATP@AgNPs,wild-type pseudorabies virus,gE-deleted vaccine,lateral flow assay,Surface-enhanced Raman spectroscopy
更新于2025-09-10 09:29:36