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Effect of the Photodynamic Therapy Applications with Potent Microalgae Constituents on Several Types of Tumor
摘要： Background: In recent years, microalgae (MA) have attracted much interest considering their possible therapeutic application. They contain active natural compounds or derivatives (extracts, pure or chemically modified compounds) that have increasing applications in the pharmaceutical industry. Methods: The present study aims to examine microalgae for new photosensitizers, with a potential to be used in the light-associated treatment of tumors. Semi-purified extracts of several microalgae strains were evaluated as photosensitizers for photodynamic therapy (PDT) applications. Four tumor cell lines (A549, LNCap, MCF-7, and MDA-MB 435) were used to assess 34 samples extracted by three methods: cellulase enzyme, lysozyme enzyme and ultra-sonication. The fluorescence measurements and the recorded images alongside the spectral intensities between 650–800 nm wavelengths provided characteristic features to some of the contents of the examined extracts. Results: Several microalgae constituents activated by blue light (BL), red light (RL) or both (in sequence) exhibited significant effects on the viability of the tumor cell lines, decreasing it as much as 95% for certain MA constituents. Majority of the MA constituents showed a higher phototoxicity after exposure to both blue and red lights than the photo-induced toxicity when exposed to a single light source. The viability of the tumor cells exhibited the dose dependent response with the MA constituents. Conclusion: The results clearly showed that MA constituents are potential photosensitizers that have a significant photo-damage effects on the tested cancer cells.
关键词： Anticancer agent,Microalgae,Enzymatic extraction,Photodynamic therapy,Photosensitizers
更新于2025-09-23 15:23:52
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[IEEE 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Cleveland, OH, USA (2018.10.17-2018.10.19)] 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Development of a Portable Intraoral Camera and a Smartphone Application for Oral Cancer PDT Treatment Guidance and Monitoring
摘要： Oral cancer presents a public health crisis, particularly in regions where widespread use of tobacco, betel quid and acacia extract lead to disproportionately high incidences of oral malignancies. Photodynamic therapy (PDT) has shown to work efficiently in early-stage oral cancer lesions; however, physicians require a method capable of assisting them during therapy delivery, so treatments can be more effective. Several studies explore how to take advantage of photosensitized fluorescent lesions to perform imaging with the purpose of guiding and monitoring patients undergoing PDT; however, these streamlined systems for intraoral PDT treatment guidance and monitoring are needed in order for this approach to achieve optimal outcomes. This paper proposes the development of a smartphone application capable of processing images taken with an endoscopy camera directly connected to the device. PDT guidance and monitoring can be simplified, and treatment could be delivered more efficiently, increasing the chance of achieving a complete tumor response.
关键词： intraoral camera,smartphone application,image processing,android development,photodynamic therapy,Oral cancer,fluorescence
更新于2025-09-23 15:23:52
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Physico-mechanical and antimicrobial properties of an orthodontic adhesive containing cationic curcumin doped zinc oxide nanoparticles subjected to photodynamic therapy
摘要： Background: Potential complications on the crown level during fixed orthodontic procedures are white spot, enamel demineralization and the tooth decay. This study evaluated the antimicrobial properties of an orthodontic adhesive incorporating cationic curcumin doped zinc oxide nanoparticles (cCur/ZnONPs), which can have the highest concentration of cCur/ZnONPs and shear bond strength (SBS) value simultaneously, on the against cariogenic bacteria including Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus acidophilus. Materials and methods: Following synthesis and confirmation of cCur/ZnONPs, SBS and adhesive remnant index (ARI) of the test adhesives containing cCur/ZnONPs (1.2, 2.5, 5, 7.5, and 10% wt.) were measured using universal testing machine and stereomicroscope, respectively. After continuously rinsed (up to 180 day), the residual antimicrobial ability of modified adhesives which can have the highest concentration of cCur/ZnONPs and SBS value simultaneously were determined by disc agar diffusion (DAD), biofilm formation inhibition, and metabolic activity assays following photo-activation using light-emitting diode (LED) for 5 min against multispecies cariogenic biofilm-producing bacteria. Results: Adhesive with 7.5% wt. cCur/ZnONPs showed the highest concentration of cCur/ZnONPs and SBS value (14.89 ± 3.26 MPa, P < 0.05) simultaneously. No significant differences in ARI scores were found between the modified adhesive and control (Transbond XT without the cCur/ZnONPs). 7.5% wt. cCur/ZnONPs following photo-activation was not colonized by the test microorganisms and suppressed 100% metabolic activity of the test microorganisms up to 90 day compared to the control group (cCur/ZnONPs free LED irradiation; P < 0.05). In DAD assay, the reduction of photodynamic disinfection of the 7.5% wt. cCur/ZnONPs against test bacteria was positively associated to the time, in such a way that it was decreased significantly after 60 day. From days 120 onwards, microbial biofilm formation and metabolic activity was progressively increased on 7.5% wt. cCur/ZnONPs adhesive discs compared to the control group (cCur/ZnONPs free LED irradiation). Conclusions: Our findings highlight the photo-activated 7.5% wt. cCur/ZnONPs can serve as an orthodontic adhesive additive for control the cariogenic multispecies biofilm, and also to reduce their metabolic activity.
关键词： Cariogenic bacteria,Orthodontic adhesive,Antimicrobial photodynamic therapy,Cationic curcumin,Zinc oxide nanoparticles
更新于2025-09-23 15:23:52
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Comparison of different non-invasive diagnostic techniques used for HMME-PDT in the treatment of port wine stains
摘要： Background: Hemoporfin-mediated photodynamic therapy (HMME-PDT) is an effective method for treating port wine stains (PWS). While, it still lacks methods to evaluate the treatment of HMME-PDT for PWS effectively and objectively. Objective: This study aimed to compare the value of different non-invasive diagnostic techniques used for HMME-PDT treatment evaluation in PWS. Methods: Thirty-one lesions of 22 patients with PWS were treated with HMME-PDT. Four non-invasive diagnostic techniques including VISIA-CRTM system, dermoscopy, high-frequency ultrasound (HFUS) and laser speckle imager (LSCI) were used to obtain standard radiographic data containing skin color, skin thickness, blood vessel morphology, blood vessel distribution and blood perfusion from lesions and normal skin surrounded before and after HMME-PDT. Results: The standard image pattern of VISIA-CRTM system showed color change of the lesions of PWS after HMME-PDT. RBX-red image of VISIA-CRTM system showed that erythema was highly aggregated even on invisible lesions at baseline but decreased after HMME-PDT. The erythema index reduced value d was related to the efficacy rating (γ=0.631, P＜0.05). Dermoscopy showed that the number of spot-like and irregular linear vessels increased correlated with increase of clinical classification. After HMME-PDT, vascular rupture was observed by dermoscopy. The response rate of lesions with vascular rupture was 100.00% (20/20). And the response rate of lesions without vascular rupture was 63.64% (7/11). Vascular rupture sign was correlated with better efficacy (P＜0.05). High-frequency ultrasound showed that dermis of PWS thickened and arranged loosely with scattered linear hypoechoic signal. After HMME-PDT, the dermal layer of the lesions became thinner with the linear hypoechoic signal decreased. The response rate of lesions with linear hypoechoic signal was 76.92% (10/13), and that without linear hypoechoic signal was 94.44% (17/18). The lesions without linear hypoechoic signal in dermis showed better efficacy (P＜0.05). In some lesions, Laser speckle contrast showed high blood perfusion signal in PWS lesions and a blood perfusion reduction after HMME-PDT. Conclusion: VISIA-CRTM system can be used to observe not only visible but also invisible lesions of PWS. Moreover, lesions fading after HMME-PDT treatment can be described objectively by VISIA-CRTM system. Dermoscopy played an important role in the clinical classification of PWS, including assessing vascular injury after HMME-PDT, guiding the adjustment of therapeutic dose, and selecting the end point of treatment. HFUS and LSCI can be both used to assist the treatment response evaluation of HMME-PDT.
关键词： dermoscopy,port-wine stains,laser speckle contrast imaging,hemoporfin-mediated photodynamic therapy,VISIA-CRTM system,high-frequency ultrasound
更新于2025-09-23 15:23:52
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Plum-blossom needle assisted photodynamic therapy: The therapy option for the treatment of oral potentially malignant disorder in the elderly
摘要： Oral leukoplakia (OLK) is one of the most common oral potentially malignant disorders (OPMD) with complex causes, a long disease course and a high tendency for recrudescence. Although a variety of methods exist for treating this disease, canceration rates remain high. Herein, we described a case of 72-year-old male patient with OLK of the palatine mucous membrane who had achieved complete remission after being treated with five sessions of plum-blossom needle (PBN) assisted 5-aminolevulinic acid-photodynamic therapy (ALA-PDT). The patient had since been subsequently placed under close observation (>12 mo). To date, there has been no recurrence. PBN assisted PDT might be suitable for the treatment of OPMDs in patients presenting with epithelial hyperkeratosis.
关键词： Plum-blossom needle,Oral potentially malignant disorders,Photodynamic therapy,Aminolevulinic acid,Hyperkeratosis,Oral leukoplakia
更新于2025-09-23 15:23:52
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Novel scintillating nanocomposite for X-ray induced photodynamic therapy
摘要： We synthesize a novel prospective nanocomposite material for X-ray induced photodynamic therapy. ZnO:Ga core is coated by the SiO2 shell facilitating a functionalization by a photosensitizer, protoporphyrin PpIX layer. By steady-state and time resolved spectroscopy we confirm the presence of energy transfer between the core and the photosensitizer, an essential requirement to induce a cytotoxic effect. Using the commercial chemical probe we also demonstrate the ability of prepared ZnO:Ga@SiO2-PpIX nanocomposite to generate singlet oxygen. Presented results show that this nanocomposite can be considered as a good candidate for potential application in X-ray induced photodynamic therapy.
关键词： Scintillator,Nanocomposite,Zinc oxide,Photodynamic therapy,Singlet oxygen
更新于2025-09-23 15:23:52
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ROS mediated antibacterial activity of photoilluminated riboflavin: A photodynamic mechanism against nosocomial infections
摘要： Nosocomial infections are a major threat to modern therapeutics. The major causative agent of these infections is multidrug-resistant gram-negative bacteria, which impart high morbidity and mortality rate. This has led to an urge for the development of new antibiotics. Antimicrobial photodynamic therapy is a promising strategy to which till date no resistant strain has been reported. Since the efficacy of photodynamic therapy largely depends on the selection and administration of an appropriate photosensitizer, therefore, the realization of clinically active photosensitizers is an immediate need. Here, by using E. coli as a study model we have demonstrated the antimicrobial photodynamic potential of riboflavin. Intracellular ROS formation by DCFH-DA assay, lipid peroxidation, protein carbonylation, LDH activity was measured in treated bacterial samples. Enzymatic (SOD, CAT, GSH) antioxidants and non-enzymatic (GSH) was further evaluated. Bacterial death was confirmed by colony forming assay, optical microscopy and scanning electron microscopy. The treated bacterial cells exhibited abundant ROS generation and marked increment in the level of oxidative stress markers as well as significant reduction in LDH activity. Marked reduction in colony forming units was also observed. Optical microscopic and SEM images further confirmed the bacterial death. Thus, we can say that photoilluminated riboflavin renders the redox status of bacterial cells into a compromised state leading to significant membrane damage ultimately causing bacterial death. This study aims to add one more therapeutic dimension to photoilluminated riboflavin as it can be effectively employed in targeting bacterial biofilms occurring on hospital wares causing several serious medical conditions.
关键词： ROS,Antibacterial,Riboflavin,Photodynamic therapy,Nosocomial infection
更新于2025-09-23 15:23:52
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Hyperbaric oxygen combined with 5-aminolevulinic acid photodynamic therapy inhibited human squamous cell proliferation
摘要： The photodynamic therapy (PDT) depends on the presence of molecular oxygen. Thus, the efficiency of PDT is limited in anoxic regions of tumor tissue and vascular shutdown. It is reported the use of hyperbaric oxygen (HBO) may enhance the efficiency of PDT. However, there are rarely studies about utilizing HBO plus PDT for treatment with human squamous cell carcinoma (SCC). Therefore, this study aimed to investigate and compare the therapeutic effect of combined therapy and PDT alone treatment. Multiple cellular and molecular biology techniques were used in the current study such as CCK-8, western blotting, flow cytometry, MDC staining and immunofluorescence assay. The results of combination index indicated that HBO combination with PDT synergistically inhibited A431 cells proliferation in vitro. In addition, we found that HBO significantly enhanced PDT-induced cell apoptosis via increasing the active caspase-3, active caspase-9, Apaf-1 and Bax levels and down-regulating Bcl-2. Meanwhile, the result of MDC and immunofluorescence assay confirmed that HBO increased PDT-induced autophagosome formation in A431 cells. Interestingly, autophagy inhibitor 3-methyladenine (3-MA) further increased combination-induced cell apoptosis by increasing the levels of active-caspase 9 and Apaf-1. Our results showed that HBO combined with PDT markedly induced A431 cells apoptosis and autophagy. Nevertheless, autophagy play a pro-survival role against apoptosis. Thus, HBO combination with PDT may constitute a promising approach to treat human squamous cell carcinoma in the future.
关键词： human squamous cell carcinoma,5-aminolevulinic acid photodynamic therapy,apoptosis,autophagy,hyperbaric oxygen
更新于2025-09-23 15:23:52
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Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells
摘要： Methylene blue (MB) is a widely used dye and photodynamic therapy (PDT) agent that can produce reactive oxygen species (ROS) after light exposure, triggering apoptosis. However, it is hard for the dye to penetrate through the cell membrane, leading to poor cellular uptake; thus, drug carriers, which could enhance the cellular uptake, are a suitable solution. In addition, the defective vessels resulting from fast vessel outgrowth leads to an enhanced permeability and retention (EPR) effect, which gives nanoscale drug carriers a promising potential. In this study, we applied poly(12-(methacryloyloxy)dodecyl phosphorylcholine), a zwitterionic polymer-lipid, to self-assemble into liposomes and encapsulate MB (MB-liposome). Its properties of high stability and fast intracellular uptake were confirmed, and the higher in vitro ROS generation ability of MB-liposomes than that of free MB was also verified. For in vivo tests, we examined the toxicity in mice via tail vein injection. With the features found, MB-liposome has the potential of being an effective PDT nano agent for cancer therapy.
关键词： methylene blue,zwitterion,liposome,photodynamic therapy,breast cancer cell
更新于2025-09-23 15:23:52
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Photodynamic therapy versus endoscopic submucosal dissection for management of patients with early esophageal neoplasia: a retrospective study
摘要： Background: Photodynamic therapy (PDT) and endoscopic submucosal dissection (ESD) have been proposed as a treatment for early esophageal neoplasia. The objective of this study is to compare between the clinical outcome after ESD and PDT to reach the best management for early esophageal neoplasia. Methods: All patients undergoing ESD or PDT for early esophageal neoplasia between 2014 and 2015 were eligible for the study. A retrospective analysis for comparison between the results of ESD and PDT was done. Results: 36 patients underwent ESD and Thirty PDT. No significant difference was found between the two groups regarding the demographic or pathologic data. Also, there was no significant difference regarding the length of hospital stay, presence of hydrothorax, fever, and pain. Operative time was significantly longer in ESD than in PDT (72 vs. 8 minutes, P<0.001). In addition, bleeding was significantly lower in ESD than PDT (12 vs. 2, P<0.05). There was a significant difference regarding stricture and cost which were less in ESD (6 vs. 15, P<0.05). However, perforation was much more in ESD (6 vs. 0, P<0.05). There was no significant difference between the two groups regarding the disease free survival (DFS), but it was observed that patients who underwent PDT had more favorable 2-year DFS rates than patients received ESD. Conclusions: The PDT may be comparable to the ESD. With the exception of esophageal stenosis, PDT could reduce many complications and have longer DFS in comparison with ESD. PDT is feasible for patients with early esophageal neoplasia confined to the mucosal layer without regional lymph nodal or distant metastasis.
关键词： Photodynamic therapy (PDT),early esophageal neoplasia,endoscopic submucosal dissection (ESD)
更新于2025-09-23 15:23:52