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A SERS aptasensor for sensitive and selective detection of bis(2-ethylhexyl)phthalate
摘要： Bis(2-ethylhexyl)phthalate (DEHP) is an endocrine disruptor commonly present in plastic products, such as PVC tubes and water bottles. In this work, a surface enhanced Raman spectroscopy (SERS) based aptasensor was developed and utilized for rapid, easy, sensitive, and specific detection of trace DEHP. A DEHP aptamer was immobilized on magnetic particles. Raman reporter molecule conjugated silver nanoparticles were clustered and coated with silica to provide a stable SERS signal. The SERS silica particle was then functionalized with 1,2,4-benzenetricarboxylic acid 1,2-bis(2-ethylhexyl) ester to increase its affinity to the DEHP aptamer. In the presence of a sample with DEHP, the high-affinity SERS silica particle competes with the DEHP molecule to bind with the aptamer on the magnetic particle. By measuring the signal of free SERS silica particles in the supernatant after magnetic separation, the concentration of DEHP in the sample was quantitatively determined. The developed DEHP aptasensor had a detection range from 0.008 to 182 nM and a limit of detection (LOD) of 8 pM. The aptasensor also showed high selectivity when exposed to interferents with analogous structures. The aptasensor was successfully tested for the detection of DEHP spiked in tap water, bottled water, and a carbonate beverage. The developed SERS-based aptasensor provides a rapid, sensitive, and easy-to-use method for the quantitative detection of DEHP in environmental and food analysis.
关键词： silica particles,SERS,competitive binding,detection,DEHP,aptasensor
更新于2025-09-19 17:15:36
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Adsorption of Trans-Zeatin on Laser-Ablated Gold Nanoparticles for Transport into Plant Cells and Growth Stimulation
摘要： Gold nanoparticles can serve as nanovectors for trans-zeatin, a natural cytokinin used in plant culture to stimulate growth and bud formation. Here, we have used Raman scattering, X-ray Photoelectron Spectroscopy, UV-vis absorption, Transmission Electron Microscopy and Density Functional Theory calculations to explain the role of citrate anions in both the formation and the reactivity of the gold colloidal nanoparticles obtained by laser ablation and to go deeper inside into the interaction between metal and zeatin. In particular, the SERS (surface-enhanced Raman scattering) spectroscopy has been employed to study the adsorption of zeatin on different gold colloidal substrates. It is established that the molecule links to gold as tautomer N7(H), predominant in the aqueous suspension, in close similarity with adenine adsorbed on gold. Moreover, the laser ablation of a gold target in citrate solution allows obtaining small, stable and reactive Au nanoparticles, able to transport zeatin inside plant cells, where it can act as a growth hormone.
关键词： gold colloid,DFT,zeatin,laser ablation,SERS
更新于2025-09-19 17:13:59
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A Highly Sensitive SERS and RRS Coupled Di-Mode Method for CO Detection Using Nanogolds as Catalysts and Bifunctional Probes
摘要： Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di‐mode method was developed for CO, based on gold nano‐enzyme catalysis and gold nanoprobes. CO can react with HAuCl4 to generate gold nanoparticles (AuNPs) in pH 5.2 HAc‐NaAc buffer. The generated AuNPs exhibited SERS activity at 1620 cm‐1 in the presence of Vitoria blue B (VBB) molecular probes, and an RRS peak at 290 nm. Based on the AuNP bifunctional probes, the increased SERS and RRS intensities respond linearly with the concentration of CO in the range of 100–1500 ng/mL and 30–5230 ng/mL, respectively. To improve the sensitivity, the produced AuNPs were used as nano‐enzyme catalysts for the new indicator reaction of HAuCl4‐ethanol (En) to amplify the signal. The sensitive SERS method was coupled with the accurate RRS method to develop a sensitive and accurate SERS/RRS di‐mode method for determination of 3.0–413 ng/mL CO, based on the AuNP‐HAuCl4‐En nanocatalytic reaction and its product of AuNPs as SERS and RRS bifunctional probes.
关键词： coupled di‐mode,SERS,nanocatalysis,CO,RRS
更新于2025-09-19 17:13:59
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Integrating SERS and PSI-MS with Dual Purpose Plasmonic Paper Substrates for On-Site Illicit Drug Confirmation
摘要： Forensic laboratory backlogs are replete with suspected drug samples. Shifting analysis towards the point of seizure would save significant time and public funds. Moreover, a two-tiered identification strategy for controlled substance testing that relies on two independent, discerning methods could entirely circumvent the need for forensic laboratory testing. To this end, we coupled Raman spectroscopy and paper spray ionization mass spectrometry (PSI-MS) on a single instrumental platform. Both methods are capable of ambient analysis with fieldable instruments, yet Raman is often limited to bulk analysis. Critical to this work is the development of a gold nanoparticle (AuNP) embedded paper swab to extend the capability of Raman spectroscopy to trace evidence via surface-enhanced Raman scattering (SERS). Plasmonic papers are characterized with respect to SERS signals and compatibility with PSI-MS analysis. Proof-of-principle is established with the identification of five representative drugs, and detection limits on the scale of 1 – 100 ng are achieved for both PSI-MS and SERS. The integrated SERS-PSI-MS system achieved 99.8% accurate chemical identification in a blind study consisting of 500 samples. Additionally, we demonstrate facile discrimination of several JWH-018 isomers via SERS even when MS and MS2 spectra are indistinguishable. Successful coupling of SERS and PSI-MS to enable on-site chemical analysis by two independent methods can potentially lead to a desirable paradigm shift in the handling of drug evidence.
关键词： PSI-MS,plasmonic paper,on-site analysis,illicit drug confirmation,SERS
更新于2025-09-19 17:13:59
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Plasmonic Molybdenum Tungsten Oxide Hybrid with Surface-Enhanced Raman Scattering Comparable to that of Noble Metals
摘要： The Surface-enhanced Raman Scattering (SERS) research is in full swing owing to its high sensitivity and high selectivity, however, the substrate with a superexcellent performance for SERS is largely confined to noble metals (Au and Ag, etc.). Although the SERS active substrate has been extended to semiconductor and transition metal, it is frustrating that their sensitivity is insufficient for widespread practical application. Here we report the plasmonic molybdenum tungsten oxide hybrid (MWO) nanomaterials (NMs) which can be used as a high-performance substrate with noble metal-comparable SERS. MWO NMs can achieve the trace detection of rhodamine 6G (R6G), basic fuchsin (BF), and oil red O (ORO). The detection limit concentration for R6G is 10-8 M with the maximum enhancement factor is up to 6.09×107. The superexcellent SERS performance was put down to the cooperative enhancement effect of electromagnetic enhancement mechanism (EM) and the charge transfer mechanism (CT). Moreover, in the proposed system, the EM and CT contribution was distinguished by employing the PVP, which serves as a barrier layer to prevent the CT process from MWO NMs to R6G. This remarkable MWO NMs can be obtained with a facile method and this research shows a new insight on the non-noble metal-based SERS substrate.
关键词： Molybdenum tungsten oxide hybrid,R6G,Noble metal-comparable,SERS,Plasmonic
更新于2025-09-19 17:13:59
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Overcurrent Electrodeposition of Fractal Plasmonic Black Gold with Broad-Band Absorption Properties for Excitation-Immune SERS
摘要： The dependence of plasmon resonance on the size, shape, and interparticle spacing of single, isolated nanostructures inherently limits their light-harvesting capability to a narrow spectral band. Here, we report a facile overcurrent electrodeposition strategy to prepare fractal plasmonic black gold (B-Au) with broad-band absorption properties (over 80% throughout the range of 300?1800 nm). The broad-band absorption properties are attributed to the excitation of multiple plasmons in the B-Au, which results in strong light?matter interaction over a broad-band spectral window. Consequently, the B-Au can produce strong broad-band surface-enhanced Raman scattering (SERS) regardless of the excitation light used. These ?ndings demonstrate that the fractal B-Au allows e?cient utilization of broad spectral photons and opens up exciting opportunities for highly sensitive SERS detection, photocatalysis, and photovoltaic devices.
关键词： broad-band absorption,surface-enhanced Raman scattering,fractal plasmonic black gold,photocatalysis,SERS,photovoltaic devices
更新于2025-09-19 17:13:59
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Porous POM/PLLA membranes decorated with gold nanoparticles as flexible and efficient plasmonic substrates for surface-enhanced Raman scattering
摘要： In this work, we have developed a flexible and efficient Surface-enhanced Raman scattering (SERS) substrate by a facile way of fabricating gold nanoparticles (NPs) decorated Poly(oxymethylene)/poly(L-lactic acid) (POM/PLLA) membranes. Firstly, POM/PLLA membranes were aminolyzed by1,6-hexanediamine to render the membranes with amino groups and porous structure simultaneously. The membranes were then immersed in tetrachloroauric acid (H4AuCl4) solution, where the AuCl4 anions were able to complex with the free amine groups of POM/PLLA, followed by in situ reduction by sodium borohydride to immobilize Au NPs on POM/PLLA membranes. The POM/PLLA/Au membranes exhibited high SERS sensitivity as well as good Raman signal reproducibility for rhodamine 6G (R6G). The minimum detection concentration was 10?14 M and the enhancement factor was calculated to be 1.54 × 109.
关键词： Poly(L-lactic acid),Flexible,SERS substrate,Membranes,Poly(oxymethylene)
更新于2025-09-19 17:13:59
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SERS-based molecularly imprinted plasmonic sensor for highly sensitive PAH detection
摘要： A novel hybrid plasmonic platform based on the synergetic combination of a molecularly imprinted polymer (MIP) thin film with Au nanoparticles (NPs) assemblies, noted as Au@MIP, was developed for surface-enhanced Raman scattering (SERS) spectroscopy recognition of polycyclic aromatic hydrocarbons (PAHs). While the MIP trapped the PAH close to the Au surface, the plasmonic NPs enhanced the molecule Raman signal. The Au@MIP fabrication comprises a two-step procedure, first, the layer-by-layer deposition of Au NPs on glass and their further coating with a uniform MIP thin film. Profilometry analysis demonstrated that the thickness and homogeneity of the MIP film could be finely tailored by tuning different parameters such as prepolymerization time or spin-coating rate. Two different PAH molecules, pyrene or fluoranthene, were used as templates for the fabrication of pyrene- or fluoranthene-based Au@MIP substrates. The use of pyrene or fluoranthene, as a template molecule to fabricate the Au@MIP thin films, enabled its ultradetection in the nM regime with a 100-fold improvement compared with the non-imprinted plasmonic sensors (Au@NIPs). The SERS data analysis allows to estimate the binding constant of the template molecule to the MIP. The selectivity of both pyrene- and fluoranthene-based Au@MIPs was analyzed against three PAHs of different sizes. The results displayed the important role of the template molecule used for the Au@MIPs fabrication in the selectivity of the system. Finally, the practical applicability of pyrene-based Au@MIPs is shown by performing the detection of pyrene in two real samples: creek water and seawater. The design and optimization of this type of plasmonic platform pave the way for the detection of other relevant (bio)molecules in a broad range of fields such as environmental control, food safety or biomedicine.
关键词： Molecularly imprinted polymers,plasmonic sensors,polycyclic aromatic hydrocarbons,SERS,hybrid nanostructures,environmental analysis
更新于2025-09-19 17:13:59
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Plasmonic cellulose textile fiber from waste paper for BPA sensing by SERS
摘要： Flexible plasmonic Surface-enhanced Raman scattering (SERS) substrates were fabricated using cellulose textile fibers, in which the textile fibers were recycled from waste paper in an eco-friendly way. The Glycidyltrimethylammonium chloride (GTAC) with positive charges was grafted onto the surface of the cellulose textile fibers through cationization. Plasmonic silver nanoparticles (Ag NPs) with negative charges were decorated onto the cellulose textile fibers via electrostatic interactions. After cationization, the variation range of the diameter of the cellulose textile fibers was significantly increased because part of the cellulose was dissolved under alkaline condition, leading to more ‘hot spots’ for SERS during the shrinking process. The cellulose textile fiber-Ag NPs nanocomposite was employed for monitoring bisphenol A (BPA) in water and soft drink by SERS and the sensitivity of BPA detection achieved 50 ppb. The recovery values of BPA in soda water samples were from 96% to 105%. These results illustrate that the cellulose textile fiber-Ag NPs nanocomposite can be used as flexible, high sensitivity SERS substrates for detecting harmful ingredients in food or environment.
关键词： flexible sensor,BPA,Cellulose textile fiber,SERS,plasmonic NPs
更新于2025-09-19 17:13:59
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Hierarchical Laser-Patterned Silver/Graphene Oxide Hybrid SERS Sensor for Explosive Detection
摘要： We demonstrate an ultrafast laser-ablated hierarchically patterned silver nanoparticle/graphene oxide (AgNP/GO) hybrid surface-enhanced Raman scattering (SERS) substrate for highly sensitive and reproducible detection of an explosive marker 2,4-dinitrotoluene (2,4-DNT). A hierarchical laser-patterned silver sheet (Ag?S) is achieved by ultrafast laser ablation in air with pulse energies of 25, 50, and 100 μJ. Multiple laser pulses at a wavelength of 800 nm and a pulse repetition rate of 50 fs at 1 kHz are directly focused on Ag?S to produce and deposit AgNPs onto Ag?S. The surface morphology of ablated Ag?S was evaluated using atomic force microscopy, optical pro?lometry, and ?eld emission scanning electron microscopy (FESEM). A rapid increase in the ablation rate with increasing laser energy was observed. Selected area Raman mapping is performed to understand the intensity and size distribution of AgNPs on Ag?S. Further, GO was spin-coated onto the AgNPs produced by ultrafast ablation on Ag?S. The hierarchical laser-patterned AgNP/GO hybrid structure was characterized using FESEM, high-resolution transmission electron microscopy, X-ray di?raction, Fourier transform infrared spectroscopy, and Raman spectroscopy. Further, hierarchical laser-patterned AgNP/GO hybrid structures have been utilized as SERS-active substrates for the selective detection of 2,4-DNT, an explosive marker. The developed SERS-active sensor shows good stability and high sensitivity up to picomolar (pM) concentration range with a Raman intensity enhancement of ～1010 for 2,4-DNT. The realized enhancement of SERS intensity is due to the cumulative e?ect of GO coated on Ag?S as a proactive layer and AgNPs produced by ultrafast ablation.
关键词： silver nanoparticle/graphene oxide (AgNP/GO) hybrid,ultrafast laser ablation,explosive detection,surface-enhanced Raman scattering (SERS),2,4-dinitrotoluene (2,4-DNT)
更新于2025-09-19 17:13:59