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Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urine
摘要： 3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this paper, on the basis of anti-3-NT specific antibody, we developed a label-free indirect competitive surface plasmon resonance (SPR) immunosensor and ELISA for the detection of 3-NT. Under the optimized conditions, the SPR immunosensor can obtain a linear range of 0.17–6.07 μg/mL and a limit of detection (LOD) of 0.12 μg/mL while the ELISA can reach 0.33–9.94 μg/mL and a LOD of 0.24 μg/mL.The selectivity of 3-NT was also testified by six kinds of amino acid analogues. Besides, the developed SPR immunosensor was compared thoroughly with a conventional ELISA in spiked analysis of urine samples. Good recoveries and correlation between these two methods were observed (R2 = 0.964). Therefore, it is concluded that the automated SPR platform can be applied to quantify 3-NT in biological samples with its sensitivity, accuracy, and real-timing.
关键词： ELISA,Human urine,3-nitrotyrosine,Surface plasmon resonance (SPR)
更新于2025-09-09 09:28:46
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Specific electron-transfer and surface plasmon resonance integrated boosting visible-light photoelectrochemical sensor for 4-chlorophenol
摘要： Emerging analytical technologies are being developed to provide advanced methods for monitoring 4-chlorophenol (4-CP) in the environment. Herein, a label-free, sensitive, and rapid photoelectrochemical (PEC) 4-CP detection system was constructed based on ternary composites of Ag nanoparticles, graphitic carbon nitride (GCN) and carbon spheres (CS) (Ag/GCN/CS). The proposed ternary composites were synthesized by a simple hydrothermal method. In this process, ascorbic acid (AA) played a crucial role in reducing Ag+ to metallic Ag, and provided a carbon source for CS. Due to synergistic promotion by the surface plasmon resonance (SPR) effect of Ag nanoparticles and electron-transfer behavior of CS, the ternary composites exhibited a broad visible light response and fast charge transfer, leading to a tremendously enhanced PEC response. Based on the promotion of the PEC response, a PEC sensor for 4-CP was designed by detecting the photocurrent signals of the PEC electrode after adding 4-CP to solution. Under optimal conditions, the PEC sensor exhibited a wide linear range for 4-CP from 16 to 1104 ng mL–1, with a limit of detection (LOD) of 5.33 ng mL–1. In particular, the present work may provide assist in better understanding the synergistic effect between the SPR effect of Ag nanoparticles and electron-transfer properties of CS. The results can also be applied to other PEC and photocatalytic systems in conjunction with high-performance photoactive materials.
关键词： PEC sensor,Carbon materials,Electron-transfer,SPR,Ternary composite
更新于2025-09-04 15:30:14
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Research on the performance of a novel SPR sensor based on graded index multimode fiber
摘要： Purpose – This paper aims to propose and demonstrate a novel surface plasmon resonance (SPR)-sensing approach by using the fundamental mode beam based on a graded index multimode fiber (GIF). The proposed SPR sensor has high sensitivity and controllable working dynamic range, which expects to solve the two bottlenecks of fiber SPR sensor, including low sensitivity and the difficulty in multichannel detection. Design/methodology/approach – The low-order mode of the GIF to SPR sense, which keeps the sensitivity advantage of the single-mode fiber SPR sensor, is used. By using this new SPR sensor, the effect of light incident angle and gold film thickness on working dynamic range was studied. According to the study results, the smaller is the incident angle, the larger is the SPR working dynamic range and the longer is the resonance wavelength with a fixed gold film thickness; the larger is the gold film thickness, the longer is the resonance wavelength with a fixed grinding angle. After the parameter optimization, the sensitivity of these two parameter-adjusting methods reach 4,442 and 3031 nm/RIU. Originality/value – When the grinding angle of the GIF increases, the dynamic range of the resonance wavelength increases and has a redshift, sensitivity increases, and the resonance valley becomes more unobvious with a fixed gold film thickness. Similarly, when gold film thickness increases, the dynamic range of the resonance wavelength increases and has a redshift, sensitivity increases, and the resonance valley becomes more unobvious with a fixed grinding angle. These adjusting performance aforementioned lay the foundation for solving of the fiber-based SPR multichannel detection and increasing of the fiber-based SPR sensor sensitivity, which has a good application value.
关键词： Dynamic range,Sensitivity,GIF,SPR sensor
更新于2025-09-04 15:30:14
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Multiplex body fluid identification using surface plasmon resonance imaging with principal component analysis
摘要： Body ?uid identi?cation is a key component in forensic casework, providing important information for the reconstruction of criminal events. Body ?uid identi?cation in combination with DNA analysis allows the linking of individuals to criminal acts and can therefore be of great importance in determining the outcome of criminal court cases. However, none of the current body ?uid identi?cation methods meet all forensic requirements, such as a high sensitivity, a high speci?city and the ability to analyse multiple body ?uids in a single run. In this pilot-study, we explore, for the ?rst time, surface plasmon resonance imaging (SPRi) with antibody-based detection to serve as a novel multiplex body ?uid identi?cation assay for blood, semen, saliva, urine and sweat using minimal sample preparation. A training set consisting of ten donors per body ?uid was analysed to determine whether body ?uid speci?c response signals could be obtained. Principal component analysis (PCA) was performed as a statistical tool to cluster the body ?uid samples by response signal pattern reduction and to uncover the sources of variation between the body ?uids. Four principal components allowed complete clustering of all body ?uid types. Blind testing of body ?uid samples revealed that ?ve out of eight unknown samples could correctly be clustered to their corresponding group, three out of eight samples were identi?ed as inconclusive. Although optimization of the current SPRi method is required for use in the forensic ?eld, this pilot-study demonstrates the feasibility of SPRi to di?erentiate ?ve forensically relevant body ?uids.
关键词： Body ?uid identi?cation,Antibody,Forensic science,Surface plasmon resonance (SPR),Principal component analysis (PCA)
更新于2025-09-04 15:30:14