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Shedding light on biocatalysis: photoelectrochemical platforms for solar-driven biotransformation
摘要： Redox biocatalysis has come to the forefront because of its excellent catalytic efficiency, stereoselectivity, and environmental benignity. The green and sustainable biotransformation can be driven by photoelectrochemical (PEC) platforms where redox biocatalysis is coupled with photoelectrocatalysis. The main challenge is how to transfer photoexcited electrons to (or from) the enzyme redox centers for effective biotransformation using solar energy. This review commences with a conceptual discussion of biocatalytic PEC platforms and highlights recent advances in PEC-based biotransformation through cofactor regeneration or direct transfer of charge carriers to (or from) oxidoreductases on enzyme-conjugated electrodes. Finally, we address future perspectives and potential next steps in the vibrant field of biocatalytic photosynthesis.
关键词： photoelectrochemical,cofactor regeneration,biotransformation,redox enzymes,solar-driven,biocatalysis,direct electron transfer
更新于2025-09-23 15:22:29
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Manipulating transition of a two-component Bose–Einstein condensate with a weak <i>δ</i> -shaped laser
摘要： Scyllo-inositol (SI), a stereoisomer of inositol, is regarded as a promising therapeutic agent for Alzheimer’s disease. Here, an in vitro cofactor-balance biotransformation for the production of SI from myo-inositol (MI) by thermophilic myo-inositol 2-dehydrogenase (IDH) and scyllo-inositol 2-dehydrogenase (SIDH) is presented. These two enzymes (i.e., IDH and SIDH from Geobacillus kaustophilus) are co-expressed in Escherichia coli BL21(DE3), and E. coli cells containing the two enzymes are permeabilized by heat treatment as whole-cell catalysts to convert MI to SI. After condition optimizations about permeabilized temperature, reaction temperature, and initial MI concentration, about 82 g L?1 of SI is produced from 250 g L?1 of MI within 24 h without any cofactor supplementation. This ?nal titer of SI produced is the highest to the authors’ limited knowledge. This study provides a promising method for the large-scale industrial production of SI.
关键词： whole-cell biotransformation,Scyllo-inositol,cofactor-free,cascade enzymes,Myo-inositol
更新于2025-09-23 15:19:57