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- 摘要
- 关键词
- 实验方案
- 产品
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Manipulating Living Systems by Light; 生命現象の光操作技術の創出;
摘要： Complex gene networks are essential for diverse biological phenomena, such as cellular programming, metabolism, homeostasis, memory formation, and circadian rhythm. To understand these biological phenomena, including diseases, and to utilize or modify them, approaches that enable optical control of the genome are required. We developed new tools for targeted gene manipulation based on optical control of the CRISPR-Cas9 system and Cre-loxP system. These tools could greatly facilitate understanding of a variety of gene functions and prove useful in biomedical applications. Genome engineering technology and optogenetics technology have emerged as different technologies from each other so far. Our studies merge these emerging research fields together.
关键词： optogenetics,photoswitching protein,cell differentiation,Cre-loxP system,genome editing,CRISPR-Cas9 system
更新于2025-09-23 15:22:29
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Neuron-like cell differentiation of hADSCs promoted by a copper sulfide nanostructure mediated plasmonic effect driven by near-infrared light
摘要： Nerve is one of the most difficult tissues to repair due to the limited source of neural stem cells and the difficulty to promote the neural differentiation of mesenchymal stem cells by growth factors. Electromagnetic field has been proved to have the ability to regulate stem cells differentiation. Although some researches promoted neural differentiation of stem cells by external power source, it is still a big challenge to realize the nerve repair in bodies because of the unwieldiness and complexity of the power supply equipment. Surface plasmon (SP) is electromagnetic oscillation caused by the interaction of free electrons and photons on metal surface, and almost no one has used this localized electromagnetic oscillation to regulate stem cells differentiation. In this study, based on the concept proposed by our group that “Regulation of stem cell fate by nanostructure mediated physical signal”, the localized electromagnetic oscillation generated by the localized surface plasmon resonance (LSPR) of copper sulfide (CuS) nanostructure irradiated by near-infrared light has been proved to have positive regulation on stem cell maturation and neuron-like cell differentiation of human adipose-derived stem cells (hADSCs). This regulation method avoids the use of wire connection of external power source, which realizes the stem cell fate regulation by external field. In addition, this work demonstrated that it is promising to realize the light promoted nerve repair in bodies by using implantable plasmonic nanomaterial with absorption in near-infrared region within human “optical window”, which has important academic value and application prospect. As we know, this is the first time to use semiconductor nanostructures as a medium to regulate stem cells neuron-like cell differentiation by near-infrared light and LSPR of plasmonic nanomaterial, which will have great influence on biomedical engineering and attract broad attention from nanomaterials scientist, neurobiologist, and neurosurgeon.
关键词： neuron-like cell differentiation,plasmonic effect,near-infrared light,hADSCs,copper sulfide nanostructure
更新于2025-09-19 17:13:59
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Dual-Enhanced Raman Scattering-Based Characterization of Stem Cell Differentiation Using Graphene-Plasmonic Hybrid Nanoarray
摘要： Surface-enhanced Raman scattering (SERS) has demonstrated great potential to analyze a variety of bio/chemical molecular interactions within cells in a highly sensitive and selective manner. Despite significant advancements, it remains a critical challenge to ensure high sensitivity and selectivity, while achieving uniform signal enhancement and high reproducibility for quantitative detection of targeted biomarkers within a complex stem cell microenvironment. Herein, we demonstrate an innovative sensing platform, using graphene-coated homogeneous plasmonic metal (Au) nanoarrays, which synergize both electromagnetic mechanism (EM)- and chemical mechanism (CM)-based enhancement. Through the homogeneous plasmonic nanostructures, generated by laser interference lithography (LIL), highly reproducible enhancement of Raman signals could be obtained via a strong and uniform EM. Additionally, the graphene-functionalized surface simultaneously amplifies the Raman signals by an optimized CM, which aligns the energy level of the graphene oxide with the target molecule by tuning its oxidation levels, consequently increasing the sensitivity and accuracy of our sensing system. Using the dual-enhanced Raman scattering from both EM from the homogeneous plasmonic Au nanoarray and CM from the graphene surface, our graphene?Au hybrid nanoarray was successfully utilized to detect as well as quantify a specific biomarker (TuJ1) gene expression levels to characterize neuronal differentiation of human neural stem cells (hNSCs). Collectively, we believe our unique graphene?plasmonic hybrid nanoarray can be extended to a wide range of applications in the development of simple, rapid, and accurate sensing platforms for screening various bio/chemical molecules.
关键词： DNA detection,Surface-enhanced Raman scattering,biosensing,stem cell differentiation,2D nanomaterials
更新于2025-09-12 10:27:22