- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Multiplexed determination of intracellular messenger RNA by using a graphene oxide nanoprobe modified with target-recognizing fluorescent oligonucleotides
摘要： A multiplexed graphene oxide (GO) fluorescent nanoprobe is described for quantification and imaging of messenger RNAs (mRNAs) in living cells. The recognizing oligonucleotides (with sequences complementary to those of target the fluorescence of the recognizing mRNAs) were labeled with different fluorescent dyes. If adsorbed on GO, oligonucleotides is quenched. After having penetrated living cells, the oligonucleotides bind to target mRNAs and dissociate from GO. This leads to the recovery of fluorescence. Using different fluorescent dyes, various intracellular mRNAs can be simultaneously imaged and quantified by a high content analysis within a short period of time. Actin mRNA acts as the internal control. This GO-based nanoprobe allows mRNA mimics to be determined within an analytical range from 1 to 400 nM and a detection limit as low as 0.26 nM. Up to 3 intracellular mRNAs (C-myc, TK1, and actin) can be detected simultaneously in a single living cell. Hence, this nanoprobe enables specific distinction of intracellular mRNA expression levels in cancerous and normal cells. It can be potentially applied as a tool for detection of cancer progression and diagnosis.
关键词： Fluorescence resonance energy transfer (FRET),Cancer biomarker,Actin mRNA,Fluorometric detection,High content analysis,Cancer diagnosis
更新于2025-09-10 09:29:36
-
Optically-Active Nanocrystals for Inner Filter Effect-Based Fluorescence Sensing: Achieving Better Spectral Overlap
摘要： Among various photophysical processes for fluorescence modulation, inner filter effect (IFE) is well-known for its simplicity and no requirement of the distances between the fluorophores and the corresponding modulators. Theoretically, the key to maximize the sensitivity of IFE-based fluorescent assays is to enlarge the overlap between the absorption of the absorber and the excitation/emission of the fluorophores. However, it is difficult to achieve perfect spectral overlap for IFE in conventional organic fluorophores with constant excitation/emission. The emergence of various optically-active nanocrystals greatly revolutionize the IFE-based fluorescent sensing. Therefore in this critical review, we first made an introduction to IFE from the viewpoint of its photophysical process. Particularly, the similarities and differences of IFE and FRET for better understanding of IFE are discussed, and the general rules for verification of IFE are proposed. The use of various optically-active nanocrystals for enhanced IFE-based sensing is especially concerned in this review.
关键词： fluorescence resonance energy transfer,Inner filter effect,spectral overlap,fluorescent sensors,optically-active nanocrystals,tunable spectra
更新于2025-09-10 09:29:36
-
A 1,8 Naphthalimide anchor Rhodamine B Based FRET Probe for Ratiometric Detection of Cr3+ion in Living Cells
摘要： A 2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)acetaldehydeanchored rhodamine B based probe, RDNAP detects Cr3+ ion by fluorescence resonance energy transfer (FRET) process in aqueous buffered acetonitrile media (7:3, v/v). Conversely, conjugation of 2-(1,3-dioxo-6-(piperidin-1-yl)-1H-benzo[de]isoquinolin-2(3H)-yl)acetaldehyde with rhodamine B provides another probe, RDNAP-PY that undergoes Cr3+assisted ratiometric fluorescence and colorimetric change in the same media. RDNAP-PY provides higher FRET efficiency and detects as low as 1.81×10?6 M Cr3+with an association constant, 15.9 × 104 M-1. Other common ions do not interfere. RDNAP-PY efficiently images intracellular Cr3+ in live Hep3B, MCF-7, HeLa, SiHa and HEK 293T cells under fluorescence microscope in a ratiometric and time dependent manner. 1H NMR titration and DFT studies strongly support experimental findings.
关键词： 1,8 Naphthalimide,Live cell imaging,Ratiometric probe,fluorescence resonance energy transfer,DFT calculation
更新于2025-09-09 09:28:46