- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
A through-bond energy transfer-based ratiometric fluorescent pH probe: For extreme acidity and extreme alkaline detection with large emission shifts
摘要： A ratiometric fluorescence pH probe 1 based on through-bond energy transfer (TBET) with a 2-(2-hydroxyphenyl)benzoxazole (HBO) as donor and a Rhodamine derivative as acceptor is developed through simple condensation reaction. The probe exhibits a ratiometric fluorescence emission (I593/I422) characteristics and linear response to extreme acidity range of 5.00-2.88, and a ratiometric fluorescence emission (I555/I422) characteristics and linear response to extreme alkaline range of 10.00-13.78, respectively. Moreover, 1 possesses highly selective response to pH over metal ions, good reversibility and excellent photostability. Probe 1 is cellpermeable and can distinguish near pH 5.55 fluctuations in Hela cells. Furthermore, 1 can be immobilized on a test paper, which shows a rapid and reversible colorimetric response to HCl/NH3 vapor by the naked-eye.
关键词： ratiometric fluorescent response,pH sensing,large emission shifts,through-bond energy transfer,fluorescent probe
更新于2025-11-21 11:24:58
-
Design and synthesis of a new fluorescent probe for cascade detection of Zn <sup>2+</sup> and H <sub/>2</sub> PO <sub/>4</sub><sup>?</sup> in water and targeted imaging of living cells
摘要： Design and synthesis of new fluorescence probes with good water‐solubility is of great importance to better understanding the significant role of ions which are related to biology and the environment. As important ions, zinc ion (Zn2+) and dihydrogen phosphate ion (H2PO4?) display essential roles in living systems, and quantitative detection of these ions in water is still a challenge. In order to consider the significant role of the galactose moiety in the design of a water‐soluble fluorescence sensor, herein, we have developed a novel probe, Gal‐AQTF, for the cascade detection of Zn2+ and H2PO4? with excellent selectivity in water. Through the introduction of the galactose moiety onto the sensor AQTF, which has been reported earlier by us, the water‐solubility, cell compatibility and targeting ability were enhanced. Gal‐AQTF has been successfully applied in the imaging of the living cells of HepG2 and A549, and illustrated good selectivity for the HepG2 cells which overly express the asialoglycoprotein (ASGP) receptor.
关键词： targeted imaging,Galactose,Zn2+ and H2PO4?,in water,fluorescent probe
更新于2025-11-21 11:24:58
-
A sequential and reversibility fluorescent pentapeptide probe for Cu(II) ions and hydrogen sulfide detections and its application in two different living cells imaging
摘要： In this study, we report a sequential and reversibility fluorescent probe (DP5) based on pentapeptide conjugated with dansyl groups using the solid phase peptide synthesis (SPPS) technology. DP5 showed immediate “turn off” response toward Cu2+ ions at an excitation wavelength of 330 nm with detection limits of 23.5 nM. The 2:1 binding ratio between DP5 and Cu2+ were confirmed using Job's plot method and fluorescence titration study, and DP5-Cu complex was observed with an association constant of 6.76 × 108 M?2. As designed, DP5-Cu complex as a promising analytical probe exhibited highly selective for H2S detection in aqueous solutions. The detection limit for H2S was obtained to be 17.2 nM, and lower than EPA and WHO guidelines. In addition, the reversibility and cyclicity were imparted to the DP5 during the detection of Cu2+ and H2S, and cycle effect is very good. Furthermore, DP5 displayed better biocompatibility and low biotoxicity, and sequential fluorescence “on-off-on” responses of DP5 to Cu2+ and H2S were successfully applied in two different living cells.
关键词： Cu2+ ions,Pentapeptide,Fluorescent probe,Cell imaging,Hydrogen sulfide,Aqueous solutions
更新于2025-11-21 11:08:12
-
A red-emitting fluorescent probe with large Stokes shift for real-time tracking of cysteine over glutathione and homocysteine in living cells
摘要： Fluorescent probes with high quality for highly selective detection of cysteine (Cys) are still urgently in demand because of the indispensable roles Cys plays in the biological systems. Herein, a red-emitting fluorescent probe CP was developed for the highly selective detection of Cys over glutathione (GSH) and homocysteine (Hcy) by incorporating the recognition unit into the 2-(2-(4-hydroxystyryl)-6-methyl-4H-pyran-4-ylidene) malononitrile (P-OH) fluorophore which is characterized by red emission, noteworthy Stokes shift, and appreciable photostability. Basically, CP demonstrated appreciable sensing performance toward Cys including short response time of 4 min, high sensitivity with approximately 147-fold emission enhancement, low detection limit of 41.696 nM, and good selectivity both in the solution and living cells, indicating its promising potential of visualizing Cys in biological systems.
关键词： Large Stokes shift,Fluorescence imaging,Cysteine detection,Fluorescent probe
更新于2025-11-19 16:56:35
-
Two pH-responsive fluorescence probes based on indole derivatives
摘要： Two new pH ?uorescent probes 2,8-bis((E)-2-(1,1-dimethyl-1H-benzo[e]indol-2-yl)vinyl)-6H,12H-5,11-methanodibenzo[b,f][1,5]diazocine (1) and 2,8-bis((E)-2-(3,3-dimethyl-3H-indol-2-yl)vinyl)-6H,12H-5,11-methanodibenzo[b,f] [1,5]diazocine (2) were designed and synthesized via ethylene bridging of the Tr?ger's Base (TB) framework and indole derivatives. As the protonation of nitrogen atoms of indole derivatives, both probes displayed the signi?cant pH-dependent spectral properties. The probing behavior toward pH variations indicated that probes exhibited excellent pH dependent behavior in the pH ranges of 2.07–7.01 (1) and 1.96–7.01 (2), especially possessing good linear response in the pH ranges of 2.50–4.00. The emissions of 1 can be reversibly switched between brilliant yellow and dark states by protonation and deprotonation. Also, the yellow-green ?uorescence emission of 2 could be quenched upon protonation. Such behaviors enable them to work as turn-o? ?uorescent pH sensors in the solution state. Furthermore, protonation and deprotonation on indole moiety nitrogen of the probe 1 and 2 provided a sensing mechanism for pH changes. In addition, both probes performed good photostability, high selectivity and excellent reversibility.
关键词： Sensors,Fluorescent probe,pH detection,Luminescence,Indole derivatives
更新于2025-11-19 16:56:35
-
An AND logic gate-based fluorescence probe for the detection of homovanillic acid, an indicator of the tumor
摘要： Homovanillic acid (HVA) is one of the major metabolites of catecholamine in human. An elevated level of HVA in urine and blood has been associated with many diseases, including catecholamine-secreting tumors. Consequently, detection of the biomarker HVA in urine is an effective strategy to detect cancer precursors and early stage cancers. In this study, a fluorescence probe is designed and fabricated by integrating lanthanide ions with metal-organic frameworks (MOFs). The fluorescence probe shows distinguished response toward HVA and exhibits ratiometric detection of HVA, rendering it an excellent candidate for sensing devices. Thus, we have designed logic gate operation integrating the probe with logical operations for the intelligent sensing of HVA. Furthermore, this type of luminesce based sensors integrated with logic gate augur for the further application in real-time early diagnosis of tumors in the future.
关键词： Rare earth,Homovanillic acid,Fluorescent probe,Logic gate,Ratiometric sensing,Metal-organic frameworks
更新于2025-11-19 16:56:35
-
A novel rhodamine-based turn-on probe for fluorescent detection of Au3+ and colorimetric detection of Cu2+
摘要： In this work, we design and synthesize the novel probe RC through introduction the 1-aza-4,13-dithia-15-crown-5 ring into the structure of rhodamine 6G hydrazide, where the N atom of crown ring is responsible for quenching of rhodamine fluorescence. The compound obtained behaves as multifunctional cation sensor providing selective fluorescent response to Au3+ and selective colorimetric response to Cu2+ ions in aqueous acetonitrile (1/1, v/v) at pH 7.0. The use of 10-5 M RC solution allowed reliable determination of target cations in the presence of a wide range of environmentally relevant ions with detection limits of 2×10-6 M and 5×10-7 M for gold and copper, respectively.
关键词： Colorimetric probe,Cu,Sensor,Rhodamine,Fluorescent probe,Au
更新于2025-11-19 16:46:39
-
A Red Emitting Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfide in Living Cells and Tissues
摘要： Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.
关键词： Hydrogen polysulfide,Fluorescent probe,Lung tissues,Living cells
更新于2025-11-19 16:46:39
-
FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H2O2/H2S formation through multicolor images
摘要： We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H2S and H2O2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λabs 390 nm) and fluorescence emission (λem 460 nm) of probe PHS1 in the presence of H2O2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H2S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H2O2, H2S, and H2O2/H2S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H2S, H2O2) in cancer cells.
关键词： FRET-based fluorescent probe,Naphthalimide,Endogenous H2O2,Endogenous H2S,Coumarin
更新于2025-11-19 16:46:39
-
A fluorescent probe for sequential sensing of MnO <sub/>4</sub><sup>?</sup> and Cr <sub/>2</sub> O <sub/>7</sub><sup>2?</sup> ions in aqueous medium based on a UCNS/TMB nanosystem
摘要： This paper reports the successful design of an UC fluorescence nanosystem for distinguishable and sequential detection of MnO4? and Cr2O72? based on the inner filter effect (IFE) between Gd2O3:Yb,Er upconversion nanospheres (UCNS) and 3,3’,5,5’-tetramethylbenzidine (TMB). The detection of MnO4? was realized by a stoichiometric oxidation reaction between TMB and Mn(VII), which resulted in quenching of the upconversion luminescence (UCL) by the blue product through IFE. The assay of Cr2O72?, in contrast, was based on the formation of complexes and the consumption of the oxidation product of the previous reaction. The sensing of MnO4? and Cr2O72? do not interfere with each other when Pb2+ was added as a masking agent. This provides a new strategy for sensitive and selective detection of MnO4? and Cr2O72?. The detection of MnO4? showed not only a low LOD of 0.243 μM but also high selectivity over other possible interfering ions. The sensitivity assay of MnO4? can be performed in the linear range of 1.85 to 69 μM. An excellent linear relationship was also found in the sensing of Cr2O72?, with a linear range of 18.3 to 250 μM and a LOD of 2.95 μM. The selectivity towards Cr2O72? was also proved to be good. Based on these properties, the UCNS–TMB nanoplatform is a potential chemosensor for both MnO4? and Cr2O72? ions in practical assays for waste water.
关键词： upconversion nanospheres,aqueous medium,UCNS/TMB nanosystem,Cr2O72?,MnO4?,TMB,fluorescent probe,inner filter effect,sequential sensing
更新于2025-11-14 17:03:37