- 标题
- 摘要
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- 实验方案
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Activity-based Near-Infrared Fluorogenic Probe Enables In Vitro and In Vivo Profiling of Neutrophil Elastase
摘要： Neutrophil elastase (NE), a typical hematopoietic serine protease, has significant roles in inflammatory and immune responses, and thus highly associated with various diseases such as acute lung injury (ALI) and lung cancer. Rapid and accurate measurement of NE activity in biological systems is particularly important for understanding the role of NE in inflammatory diseases, as well as the clinic diagnosis. However, the specific detection and non-invasive imaging of NE in vivo remains a challenge. To address this issue, a small-molecule substrate-based near-infrared fluorogenic probe (NEP) for NE was constructed via incorporating pentafluoroethyl as the recognition group with a hemicyanine dye-based fluorophore. This initially quenched probe possesses over than 25-fold red fluorescence enhancement upon the catalysis of human NE and the detection limit is about 29.6 ng/mL. In addition, the high specificity and the long emission wavelength (λemmax = 700 nm) of NEP allowed the direct monitoring of NE-trafficking, exogenous NE up taking and endogenous NE up-regulation at the cellular level. Moreover, the successfully spatiotemporal imaging of NE in ALI model mice also made it a promising new tool in clinical diagnosis for ALI and other lung diseases.
关键词： intracellular trafficking,Neutrophil elastase,acute lung injury.,fluorogenic probe
更新于2025-09-23 15:23:52
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A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging
摘要： A fluorogenic probe for bacteria imaging was reported. The binding with anionic bacterial surfaces disassembled the self-assembly probe to turn-on the fluorescence and shift pyrene monomer/excimer ratiometric signals.
关键词： Self-assembly,Pyrene,Bacteria,Fluorogenic probe,Imidazolium
更新于2025-09-23 15:21:21
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Fast response fluorogenic probe for visualizing hypochlorite in living cells and Zebrafishes
摘要： A novel fast response and visible fluorogenic probe (D1) for monitoring hypochlorite (ClO?) has been developed based on specific ClO? removal of a C=N bond. The response of the probe to ClO? showed increases linearly, and the fluorescence intensity heighten about 25 times. D1 responses to ClO? were observed with high selectivity and sensitivity within 10 s by naked eyes. D1 can not only detect the level of hypochlorite in vitro such as urine, but also monitoring hypochlorite content in extremely cold condition as low as -78oC. Meanwhile, the good biocompatibility guaranteed the use of D1 to detect intracellular ClO? under confocal microscopy. Moreover, D1 has been successfully applied to monitor the exogenous and endogenous ClO? in Zebrafishes with fluorescence imaging.
关键词： Zebrafishes,live cell imaging,hypochlorite,fast response,fluorogenic probe
更新于2025-09-23 15:21:01
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BODIPY with Tuned Amphiphilicity as Fluorogenic Plasma Membrane Probe
摘要： Staining of the plasma membrane (PM) is essential in bio-imaging as it delimits the cell surface and provides various information regarding the cell morphology and status. Herein, the lipophilicity of a green-emitting BODIPY fluorophore was tuned by gradual functionalization with anchors composed of zwitterionic and aliphatic groups, thus yielding three different amphiphilic dyes. We found that BODIPY bearing one or three anchors failed in efficiently staining the PM: derivative with one anchor showed low affinity to PM and exhibited strong fluorescence in water due to high solubility, whereas BODIPY with three anchors aggregated strongly in media and precipitated before binding to PM. In sharp contrast, the BODIPY bearing two anchors (B-2AZ, MemBright-488) formed virtually non-fluorescent soluble aggregates in aqueous medium that quickly de-aggregated in the presence of PM leading to bright soluble molecular form (quantum yield of 0.92). This fluorogenic response allowed for efficient probing of the PM at low concentration (20 nM) with high signal to background ratio images in mono- as well as two-photon excitation microscopy. B-2AZ proved to selectively stain the PM in a more homogeneous manner than the commercially available fluorescently labelled lectin WGA. Finally, it was successfully used in 3D-imaging to reveal fine intercellular tunneling nanotubes in KB cells and to stain the PM in glioblastoma cells in spheroids.
关键词： tunneling nanotubes,two-photon imaging,spheroids,Plasma membrane probe,fluorogenic probe
更新于2025-09-04 15:30:14
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Deep-red fluorogenic probe for rapid detection of nitric oxide in Parkinson’s disease models
摘要： Nitric oxide (NO), as one of important gaseous signaling molecule in human body, has been associated with a variety of physiologic processes. However, excessive production of NO has been profoundly implicated in the pathogenesis of neurodegenerative disorders in particular, Parkinson's disease (PD). Therefore, accurate and facile detection of NO is of great significance for investigating its functions in PD, and the subsequent diagnosis and/or treatment. Herein, we developed a deep-red fluorogenic probe (BT-NH), with high sensitivity and good selectivity to detect NO, which was successfully used to visualize exogenous/endogenous NO level in living cells, and further applied to in vitro and in vivo PD models.
关键词： Parkinson’s disease,Drosophila,live cell imaging,nitric oxide,fluorogenic probe
更新于2025-09-04 15:30:14
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Fast response two-photon fluorogenic probe based on Schiff base derivatives for monitoring nitric oxide levels in living cells and zebrafish
摘要： Numerous excellent fluorogenic probes for NO detection based on NO-mediated reactions have been developed. However, some of them still suffer from limitations such as low selectivity, slow response, and a short excitation wavelength (o500 nm). Herein, a novel two-photon fluorogenic probe (XNO1) based on a Schiff base derivative has been reported for the first time. This new mechanism with a Schiff base structure as the specific response moiety towards NO endows the probe with fast responsibility, high selectivity and pH-independent properties. Furthermore, XNO1 has been demonstrated to be lysosome-targeted and to successfully monitor exogenous/endogenous NO in living cells and zebrafishes with one- and two-photon fluorescence imaging.
关键词： living cells,nitric oxide,two-photon fluorogenic probe,zebrafish,Schiff base derivatives
更新于2025-09-04 15:30:14