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Glutaraldehyde non-conjugated chitosan polymer fluorophores for selective determination of picric acid via fluorescence resonance energy transfer strategy
摘要： Water-dispersed glutaraldehyde (GA) non-conjugated chitosan polymer fluorophores (GCPF) with quantum yield of 16 % is synthesized by stirring chitosan and GA for 6 h at room temperature in the present work. It is a facile and mild method and fluorescent GCPF can be stabled for two months. Owing to the spectral overlap of fluorescent spectrum of GCPF and absorption spectrum of picric acid (PA), a novel sensitive fluorescent method using fluorescent GCPF for PA detection from 10 nM to 50 μM via fluorescence resonance energy transfer (FRET) strategy is established. The distance between donor of GCPF and acceptor of PA (R0 value) is calculated to be 3.5 nm. FRET method using fluorescent GCPF possesses high sensitivity (LOD of 2.8 nM), and selectivity and fast response within 2 min. Moreover, fluorescent GCPF is also utilized in visual analysis of PA using cotton swabs. Fluorescence quenching effect can be observed by eyes irradiated with 365 nm ultraviolet light at cotton swabs and using GCPF solid on quartz glasses, which paves an effect and wide way for the application of fluorescent GCPF in our daily life.
关键词： Glutaraldehyde non-conjugated chitosan polymer fluorophores (GCPF),picric acid (PA),fluorescence resonance energy transfer (FRET),glutaraldehyde (GA),chitosan
更新于2025-11-14 15:23:50
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A sustainable solution for removal of glutaraldehyde in saline water with visible light photocatalysis
摘要： Glutaraldehyde (GA) is the most common biocide used in unconventional oil and gas production. Photocatalytic degradation of GA in brine simulating oil and gas produced water using Ag/AgCl/BiOCl composite as a photocatalyst with visible light was investigated. Removal of GA at 0.1 mM in 200 g/L NaCl solution at pH 7 was 90% after 75 min irradiation using 5 g/L of the photocatalyst. The GA removal followed pseudo-first order reaction with a rate constant of 0.0303 min?1. At pH 5 or at 300 g/L NaCl, the photocatalytic removal of GA was almost completely inhibited. Similar inhibitions were observed when adding dissolved organic carbon (from humic acid) at 10 and 200 mg/L, or Br? at 120 mg/L to the system. The removal rate of GA markedly increased with increasing pH (5-9), photocatalyst loading (2-8 g/L) and under 350 nm UV (compared to visible light). On the contrary, the removal rate of GA markedly decreased with increasing NaCl and initial GA concentrations (0-300 g/L for NaCl and 0.1-0.4 mM for GA). A quenching experiment was also conducted; electron holes (h+) and superoxide (O2?-) were found as the main reactive species responsible for the removal of GA while ?OH had a very limited effect.
关键词： saline,Glutaraldehyde,produced water,hydraulic fracturing fluid,visible light photocatalysis
更新于2025-09-23 15:23:52
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A robust covalent coupling scheme for the development of FRET aptasensor based on amino-silane modified graphene oxide
摘要： In recent years, numerous aptamers have been physisorbed on graphene oxide (GO) to develop FRET based aptasensors based on the high fluorescence quenching efficiency of GO. However, physisorbed aptasensors show poor signal reversibility and reproducibility as well as nonspecific probe displacement and thereby, are not suitable for many analytical applications. To overcome these problems when working with complex biological samples, we developed a facile and robust covalent surface functionalization technique for GO-based fluorescent aptasensors using a well studied adenosine triphosphate (ATP) binding aptamer (ABA). In the scheme, GO is first modified with amino-silane, and further with glutaraldehyde to create available carbonyl groups for the covalent attachment of a fluorophore and an amino dual modified ABA. The surface modification method was characterized by zeta potential, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The linearity, sensitivity, selectivity and reversibility of the resulting GO based covalent aptasensor was determined and systematically compared with the physisorbed aptasensor. While both sensors showed similar performance in terms of sensitivity and linearity, better selectivity and higher resistance to nonspecific probe displacement was achieved with the developed covalent ABA sensor. The surface modification technique developed here is independent from the aptamer sequence and therefore could be used universally for different analytical applications simply by changing the aptamer sequence for the target biomolecule.
关键词： aptamer,physisorption,EDC/NHS,fluorescent aptasensor,Graphene oxide,glutaraldehyde,amino-silane,covalent conjugation
更新于2025-09-23 15:21:01