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Aprotinin Encapsulated Gold Nanoclusters: A Fluorescent Bioprobe with Dynamically Nuclear Targeting and Selective Detection of Trypsin and Heavy Metal
摘要： Fluorescence imaging has currently emerged as one of the most frequently used noninvasive imaging technology to selectively monitor biological processes in living systems. In past decades, gold nanoclusters (Au NCs) received increasing attraction because of their intrinsic fluorescence and their inherent biocompatibility. As a stabilizing and reducing agent, an abundant, sustainable and widely used polypeptide derived drug molecule, aprotinin (Ap) is selected for the synthesis of Au nanoclusters (Ap-Au NCs) due to characteristic bioactivity, excellent biocompatibility, biodegradability, nonallergenic character. Herein, Ap encapsulated Au NCs exhibiting desirable red fluorescence feature was facilely produced for the first time, which were subsequently used for cell imaging and detection of various analytes. Much interestingly, dynamically subcellular localization of Ap-Au NCs from the cytoplasm to the nucleus in Hela cells was observed. Afterward, it has shown the selective and quantitative detection of trypsin by using Ap encapsulated Au NCs. Lastly, Ap-Au NCs were readily used for detection of mercury and copper quantitatively. The photoluminescence of the Ap-Au NCs was quenched with the addition of metioned analytes. This study opens crucial insights on the integration of biomolecule with metal nanoclusters, also discusses a multifunctional nanomaterial platform for cell imaging, subcellular targeting, biosensing and drug delivery.
关键词： trypsin detection,gold nanoclusters,aprotinin,cell imaging,heavy metal detection,Fluorescence imaging
更新于2025-09-23 15:21:01
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Optical Fiber Refractometer Based Metal Ion Sensors
摘要： Research into optical fiber refractometers yielded remarkable results over the past decade. Numerous sensing schemes were proposed and demonstrated, which possessed different advantages while facing unique limitations. On top of their obvious applications in measuring refractive index changes of the ambient environment, several studies reported advanced applications of such sensors in heavy metal ion detection by means of surface coating of the refractometers with heavy metal ion sensitive materials. This paper surveys the effort these optical fiber metal ion sensors based on surface coated optical fiber refractometer, discusses different technologies and methods involved, and highlights recent notable advancements.
关键词： optical fiber technology,heavy metal detection,optical fiber sensors,chemical sensing
更新于2025-09-23 15:19:57