- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Raspberry (Breeding, Challenges and Advances) || Use of Imaging Technologies for High Throughput Phenotyping
摘要： In this chapter we describe a high throughput phenotyping system that we have developed for raspberry and other soft fruit crops and its application to against individual (water stress regimes, vine weevil and Phytophthora root rot) and combined stresses. The term phenotype is used to describe the morphology, physiology, biochemistry and ontogeny of a plant, encompassing the diverse array of traits that contribute to the plant’s functional form. Plant phenotype is expressed as a consequence of the interaction between the plant genetic background (i.e. genotype) and the biotic and abiotic conditions experienced by the plant in its growing environment. A key focus of raspberry and other crop breeding is to understand the genetic control of desirable plant traits and the influence of environmental conditions on trait expression, which relies on the ability to collect quantitative information on target traits across genetically-characterised populations of plants. The process of characterising plant traits in detail, referred to as plant phenotyping, is a major challenge when relating plant genetic information to traits for plants in realistic growing environments.
关键词： high throughput phenotyping,raspberry,soft fruit crops,plant breeding,imaging technologies
更新于2025-09-10 09:29:36
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Computational screening of bulk materials with intrinsic intermediate band
摘要： Intermediate band compounds are promising high-efficiency materials for solar cells. Besides that, they also show unusual properties that make them interesting from a fundamental point of view. In this work, we create a series of filters capable of searching the AFLOW-ICSD materials database for compounds that show intrinsic intermediate bands. We analyse all available binary and ternary compounds, totaling 45,656 entries. The selected materials show at least one narrow band (maximum width of 1 eV) next to the energy gap. After removal of repeated entries and of wide bandgap insulators, we end up with a total of 660 materials that present intermediate bands. From this group, the majority has completely empty or filled intermediate bands or, during new calculations, changed to another state with a different type of intermediate band or no band of this type. Nevertheless, three compounds fulfill all criteria and continue to present a partially filled intermediate band (Bi2Rh2O7, Ca5FeN6, and OsTb6I10), showing potential for solar cells applications.
关键词： Intermediate band,Solar cells,Materials screening,High-throughput screening,DFT calculations
更新于2025-09-10 09:29:36
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A polarization sensitive light scattering unit for high throughput screening
摘要： A simple, effective light scattering prototype sensitive to both polarized and depolarized scattering was constructed, and its performance was tested on a variety of pure liquids and optically isotropic and anisotropic polymer solutions and colloidal suspensions. The results, performance, and means to further improvement are reported here. Because of its simplicity and low cost, many identical units can be produced to construct a simultaneous multiple sample light scattering platform that can be used to monitor polymer and colloid solution stability, phase changes, aggregation, degradation, etc. Measurable depolarization was found for a variety of organic liquids and suspensions of both poly?uoroethylene and latex spheres. No detectable depolarization was found for various polymers.
关键词： light scattering,depolarized scattering,colloidal suspensions,polymer solutions,high throughput screening,polarization sensitive
更新于2025-09-10 09:29:36
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[IEEE IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Valencia, Spain (2018.7.22-2018.7.27)] IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Implementation of UAV-Based Lidar for High Throughput Phenotyping
摘要： High throughput phenotyping is rapidly gaining widespread popularity due to its ability to non-destructively extract plant traits, such as plant height, canopy density, leaf and plant structure, and so on. In this study, we focus on developing a UAV-based LiDAR system to acquire accurate time-series 3D point clouds for monitoring two specific plant traits – plant height and canopy cover – which are integral for enhancing crop genetic improvement to meet the needs of future generations. Furthermore, the obtained estimates are validated by comparing the results with those obtained from wheel-based LiDAR data.
关键词： High throughput phenotyping,UAV,plant height,canopy cover,LiDAR system
更新于2025-09-09 09:28:46
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Recent Advances in Design of Fluorescence-based Assays for High-throughput Screening
摘要： Significant improvements in disease management have been achieved in the past few decades. Using cancer as an example, survival rates have increased dramatically in economically developed countries, according to the World Cancer Report published by WHO in 2014.1 One of the highlights is the average 5-year survival rate of children diagnosed before the age of 15 years in Britain increased from less than 30% in 1966-70 to almost 80% in 1996-2000. The improvement can be attributed to the establishment of early screening and awareness programs, identification of a large number of genetic or non-genetic risk factors or markers, and availability of diverse therapeutic strategies including surgery and treatments using radioactive reagents, small molecules, or biological drugs. Still, cancer remains one of the major causes of deaths worldwide, affecting millions of people. Survival rates in developing countries are poor, and the global and regional burden of cancer in terms of both incidence and mortality keep increasing along with the growing population and aging society. Further improvement heavily relies on early diagnosis, wide access to treatment options, and discovery of more effective biomarkers as well as anticancer drugs with high efficacy. Such demands are also applied to diagnosis and cure for all other diseases, calling for rapid advancements in high-throughput screening (HTS) technologies that can efficiently test a large number of targets and samples, monitor diverse enzymatic processes in situ, and effectively identify biologically active substances.2
关键词： Fluorescence-based assays,High-throughput screening,Disease diagnosis,Drug discovery,Biomarkers
更新于2025-09-09 09:28:46
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Development of a micelle-enhanced high-throughput fluorometric method for determination of niclosamide using a microplate reader
摘要： The present paper describes the development and validation of a simple and sensitive micelle‐enhanced high‐throughput fluorometric method for the determination of niclosamide (NIC) in 96‐microwell plates. The proposed method is based on the reduction of the nitro group of niclosamide to an amino group using Zn/HCl to give a highly fluorescent derivative that was developed simultaneously and measured at λem 444 nm after excitation at λex 275 nm. Tween‐80 and carboxymethylcellulose (CMC) have been used as fluorescence enhancers and greatly enhanced the fluorescence by factors of 100–150%. The different experimental conditions affecting the fluorescence reaction were carefully investigated and optimized. The proposed method showed good linearity (r2≥ 0.9997) over the concentration ranges of 1–5 and 0.5–5 μg/ml with lower detection limits of 0.01 and 0.008 μg/ml and lower quantification limits of 0.04 and 0.03 μg/ml on using Tween‐80 and or CMC, respectively. The developed high‐throughput method was successfully applied for the determination of niclosamide in both tablets and spiked plasma. The capability of the method for measuring microvolume samples made it convenient for handling a very large number of samples simultaneously. In addition, it is considered an environmentally friendly method with lower consumption of chemicals and solvents.
关键词： high‐throughput method,microplate reader,fluorescence,niclosamide determination
更新于2025-09-09 09:28:46
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Zebrafish larvae show negative phototaxis to near-infrared light
摘要： Zebrafish larvae (Danio rerio) are among the most used model species to test biological effects of different substances in biomedical research, neuroscience and ecotoxicology. Most tests are based on changes in swimming activity of zebrafish larvae by using commercially available high-throughput screening systems. These systems record and analyse behaviour patterns using visible (VIS) and near-infrared (NIR) light sources, to simulate day (VIS) and night (NIR) phases, which allow continuous recording of the behaviour using a NIR sensitive camera. So far, however, the sensitivity of zebrafish larvae to NIR has never been tested experimentally, although being a critical piece of information for interpreting their behaviour under experimental conditions. Here, we investigated the swimming activity of 96 hpf (hours post fertilization) and 120 hpf zebrafish larvae under light sources of NIR at 860 nm and at 960 nm wavelength and under VIS light. A thermal source was simultaneously presented opposite to one of the light sources as control. We found that zebrafish larvae of both larval stages showed a clear negative phototactic response towards 860 nm NIR light and to VIS light, but not to 960 nm NIR light. Our results demonstrated that zebrafish larvae are able to perceive NIR at 860 nm, which is almost identical to the most commonly used light source in commercial screening systems (NIR at 850 nm) to create a dark environment. These tests, however, are not performed in the dark from the zebrafish′s point of view. We recommend testing sensitivity of the used test organism before assuming no interaction with the applied light source of commonly used biosensor test systems. Previous studies on biological effects of substances to zebrafish larvae should be interpreted with caution.
关键词： Zebrafish larvae,near-infrared light,high-throughput screening systems,negative phototaxis,behavioral response
更新于2025-09-09 09:28:46
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Automated Behavioral Analysis of Large <em>C. elegans</em> Populations Using a Wide Field-of-view Tracking Platform
摘要： Caenorhabditis elegans is a well-established animal model in biomedical research, widely employed in functional genomics and ageing studies. To assess the health and fitness of the animals under study, one typically relies on motility readouts, such as the measurement of the number of body bends or the speed of movement. These measurements usually involve manual counting, making it challenging to obtain good statistical significance, as time and labor constraints often limit the number of animals in each experiment to 25 or less. Since high statistical power is necessary to obtain reproducible results and limit false positive and negative results when weak phenotypic effects are investigated, efforts have recently been made to develop automated protocols focused on increasing the sensitivity of motility detection and multi-parametric behavioral profiling. In order to extend the limit of detection to the level needed to capture the small phenotypic changes that are often crucial in genetic studies and drug discovery, we describe here a technological development that enables the study of up to 5,000 individual animals simultaneously, increasing the statistical power of the measurements by about 1,000-fold compared to manual assays and about 100-fold compared to other available automated methods.
关键词： nematode library,neurodegeneration,amyloid formation,Alzheimer's disease,Drug discovery,Issue 141,phenotype-based screening,high-throughput screening,Immunology and Infection,C. elegans,large population analysis
更新于2025-09-09 09:28:46
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Strength in Numbers: Development of a Fluorescence Sensor Array for Secondary Structures of DNA
摘要： High-throughput assessment of secondary structures adopted by DNA oligonucleotides is currently hampered by the lack of suitable biophysical methods. Fluorescent sensors hold great potential in this respect; however, the moderate selectivity that they display for one DNA conformation over the others constitutes a major drawback to the development of accurate assays. Moreover, the use of single sensors impedes a comprehensive classification of the tested sequences. Herein, we propose to overcome these limitations through the development of a fluorescence sensor array constituted by easily accessible, commercial dyes. Multivariate analysis of the emission data matrix produced by the array allows to explore the conformational preferences of DNA sequences of interest, either by calculating the probability of group membership in the six predefined structural categories (three G-quadruplex groups, double-stranded, and two groups of single-stranded forms), or by revealing their particular structural features. The assay enables rapid screening of synthetic DNA oligonucleotides in a 96-well plate format.
关键词： fluorescence sensor array,G-quadruplex,high-throughput conformational analysis,multivariate analysis,DNA secondary structures
更新于2025-09-09 09:28:46
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[Methods in Molecular Biology] BCL-2 Family Proteins Volume 1877 (Methods and Protocols) || Rapid Imaging of BCL-2 Family Interactions in Live Cells Using FLIM-FRET
摘要： The Bcl-2 proteins control cell death via interchanging interactions within the Bcl-2 family. Fluorescence lifetime imaging microscopy (FLIM) is used to detect F?rster resonance energy transfer (FRET) between two fluorescent-fusion proteins in live cells. FLIM-FRET has been used to detect specific interactions and their disruption, for Bcl-2 family proteins. To date, this has been possible only in low throughput, due to the time required for serial data acquisition. We developed an automated optical system with eight parallel detectors for rapid and efficient data collection. Here we describe how to use this system for FLIM-FRET imaging of Bcl-2 family protein interactions in a 384-well plate format.
关键词： BH3 mimetic,FLIM Hyperspectral,FLIM-FRET,Bcl-2 family,mCerulean3,High throughput,Fluorescence lifetime imaging microscopy
更新于2025-09-04 15:30:14