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A Red Emitting Fluorescent Probe for Imaging Mitochondrial Hydrogen Polysulfide in Living Cells and Tissues
摘要： Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.
关键词： Hydrogen polysulfide,Fluorescent probe,Lung tissues,Living cells
更新于2025-11-19 16:46:39
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Photostable Ratiometric Two-photon Fluorescent Probe for Visualizing Hydrogen Polysulfide in Mitochondria and Its application
摘要： Hydrogen polysulfide (H2Sn) has currently attracted much research interest because it not only plays important physiological function in many biological and health-related events, but also considered as a newfound potent signal transducer. Small-molecule based ratiometric fluorescent probes have advantages in sensitivity and bio-detections but such approaches that intentionally developed for H2Sn detection expected to be mitochondria-accessible are still lacking. In this work, due to that triphenylphosphine group introduced into the molecular scaffold of naphthalimide derivative, Mito-NRT-HP was successfully applied to visualize intracellular H2Sn in mitochondria with excellent aqueous solubility, super photobleaching resistance, favorable cellular membrane permeability and good biocompatibility. This one- and two-photon fluorescent probe with high selectivity and sensitivity (LOD = 0.01 μM) evinced 70-fold enhancement of fluorescence ratio (I546 nm/I478 nm) in the presence of H2Sn over other reactive sulfur species (RSS). The experimental results also give Mito-NRT-HP the potential for mapping the H2Sn distribution in mitochondria and evaluating the H2Sn roles in more biological processes and demonstrated the practical application possibility of Mito-NRT-HP in early diagnosis of LPS-induced acute organ injury.
关键词： mitochondria,fluorescent probe,hydrogen polysulfide,ratiometric,two-photon
更新于2025-11-14 15:29:11
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A Two-Photon Ratiometric Probe for Hydrogen Polysulfide (H2Sn): Increase in Mitochondrial H2Sn Production in a Parkinson’s Disease Model
摘要： Hydrogen polysulfide (H2Sn, n>1), which is primarily generated during the crosstalk between H2S and reactive species (ROS and RNS), is receiving increasing attention in biochemical research. H2Sn is mostly generated in the mitochondria, and abnormal mitochondrial function and oxidative stress are directly related to many disorders including Parkinson's disease (PD). We now report a two-photon fluorescent probe (SPS-M1) for in situ detection of H2Sn and its application to a PD model to account the H2Sn levels. The probe exhibited selective and fast response to H2Sn along with a marked blue-to-green color change. SPS-M1 is sensitive enough to quantitative detection of endogenous H2Sn content in mitochondria using two-photon microscopy (TPM). Ratiometric TPM imaging of live neurons and brain slices using SPS-M1 revealed that H2Sn production is increased to a greater extent in the A53T α-synuclein (α-syn) overexpressing model than in the wild-type control. These findings suggest that the interactions of H2S and the increased ROS caused by α-syn overexpression may generate more H2Sn. By employing our recently published TP probe for mitochondrial H2S, we also found the relationship between the H2Sn and H2S; increased H2Sn and decreased H2S levels, indicating that H2S and H2Sn may play a significant role in the pathogenesis of PD. This result may be useful to biomedical studies, including PD.
关键词： two-photon microscopy,fluorescent probe,Parkinson’s disease,Hydrogen polysulfide,mitochondria
更新于2025-09-04 15:30:14