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Simultaneous quantitative analysis of indomethacin and benzoic acid in gel using ultra-violet-visible spectrophotometry and chemometrics
摘要： BACKGROUND: In order to manufacture pharmaceutical products, real-time monitoring in the manufacturing process is necessary, but large equipment cost is required to achieve it. OBJECTIVE: The aim of this research is to use ultra-violet-visible spectroscopy along with chemometrics procedure to simultaneously quantitative analysis of indomethacin (IMC) and benzoic acid (BA) in the gel during pharmaceutical manufacturing process. METHODS: The gel preparations were contained of 0.1–1.5% IMC, 0.015–0.225% BA, 2% carbopol? 941 and 95% ethanol solution. The calibration models were constructed using the partial least square regression (PLS). RESULTS: The relationships of the measured and predicted concentrations for both IMC and BA had linear plots. The developed PLS calibration models were used to monitor the IMC and BA concentrations during mixing of the gels by the planetary centrifugal and conventional mixers, respectively. IMC and BA were gradually dispersed, dissolved and completely homogeneous within 30 min by the centrifugal mixer. In contrast, IMC and BA were slowly dispersed, dissolved and completely homogeneous at more than 60 min by the conventional mixer. CONCLUSIONS: The ultra-violet-visible spectrophotometric method couples with multivariate chemometric techniques for quantitative data analysis were successfully applied for the simultaneous determination of major component IMC and trace component BA in the gel.
关键词： benzoic acid,indomethacin,partial least square regression,Ultra-violet-visible spectroscopy,process analysis technology,process monitoring
更新于2025-09-23 15:22:29
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Preparation and cell imaging of nitrogen-doped graphene quantum dot conjugated indomethacin
摘要： The nitrogen-doped graphene quantum dot conjugated indomethacin (N-GQD-IDM) was synthesized by an amide reaction. The results of FTIR indicated that the synthesis of N-GQD-IDM was successful. It was then co-cultured with MCF-7 cells, and obvious fluorescence was observed under a laser confocal scanning microscope. With the increase of incubation time, the material accumulated significantly in the cells and the fluorescence intensity of the cells was slightly improved. This compound could be suggested as a promising fluorescent probe in cancer cell labeling.
关键词： indomethacin,fluorescent probe,cancer cell labeling,nitrogen-doped graphene quantum dots
更新于2025-09-16 10:30:52