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Assessment of focused multivector ultraviolet disinfection with shadowless delivery using 5-point multisided sampling of patient care equipment without manual-chemical disinfection
摘要： The aim of this study was to evaluate the performance of a focused multivector ultraviolet (FMUV) system employing shadowless delivery with a 90-second disinfection cycle for patient care equipment inside and outside the operating room (OR) suite without manual-chemical disinfection. A 5-point multisided sampling protocol was utilized to measure the microbial burden on objects inside and outside the OR environment in a 3-phase nonrandomized observational study. Surface sampling was performed pre- and postdisinfection in between cases (IBCs) to assess the performance of manual-chemical disinfection. FMUV system performance was separately assessed pre- and postdisinfection before the first case and IBCs. Additionally, visibly clean high-touch objects were sampled outside the OR, and the microbial burden reductions after FMUV disinfection were quantified without manual-chemical disinfection. Manual-chemical disinfection reduced the active microbial burden on sampled objects IBCs by 52.8%-90.9% (P < .05). FMUV reduced the active microbial burden by 92%-97.7% (P < .0001) before the first case and IBCs combined, and 96.3%-99.6% (P < .0001) on objects outside the OR without chemical disinfection. Five-point multisided sampling proved effective for assessing disinfection performance on all exterior sides of equipment. FMUV produced significant overall reductions of the microbial burden on patient care equipment in all study phases and independent of manual cleaning and chemical disinfection.
关键词： Disinfectants,Surface sampling techniques,Manual cleaning,Environmental disinfection,Ultraviolet germicidal irradiation,Infection prevention
更新于2025-09-10 09:29:36
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Pattern-Based Interpretation Criteria for 18F-Fludeoxyglucose Positron Emission Tomography/Computed Tomography in the Assessment of Pyogenic Spine Infection
摘要： 18F-?udeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) is the procedure of choice for investigation of pyogenic spine infection (PSI) when other imaging fails to provide a de?nitive diagnosis, and for assessment of treatment response when patients are not responding as expected to antibiotic therapy [1]. Interpretation criteria for 18F-FDG PET/CT in the initial diagnosis of spine infection have previously relied on the intensity of the 18F-FDG activity. Sites with activity greater than in normal marrow are generally considered actively infected. Results from different series are dif?cult to compare because of different imaging techniques and inclusion criteria. PET-only systems (without the bene?t of a localization CT) were used prior to the availability of hybrid PET/CT. Patient populations were heterogeneous and included spine and peripheral osteomyelitis cases, infection with pyogenic organisms and tuberculosis, and non-operated and postoperative patients with and without hardware. Despite these shortcomings, a review of the early literature [2] and more recent series [3e6] have all reported high sensitivity and speci?city in the diagnosis of spine infection. The localization of 18F-FDG is nonspeci?c and occurs with infection but also with non-septic in?ammation, fracture, or neoplasm. The references quoted above have consisted of random cases, or prospective series of cases, of suspected spine infection. The prevalence of 18F-FDG-avid non-septic conditions in these series is likely low, explaining the relatively high speci?cities reported. False-positive 18F-FDG PET/CT using intensity-based criteria may be more common in problem cases done for initial diagnosis because imaging other than 18F-FDG PET/CT failed to provide a de?nitive diagnosis. Non-septic activity is commonly seen if 18F-FDG PET/CT is done for assessment of antibiotic treatment response for PSI.
关键词： Antibiotic treatment response,18F-?udeoxyglucose positron emission tomography/computed tomography,Pyogenic spine infection,Discitis,Vertebral osteomyelitis
更新于2025-09-10 09:29:36
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Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection
摘要： We have engineered streptavidin-labeled fluorescent gold nanoclusters to develop a gold nanocluster immunoassay (GNCIA) for the early and sensitive detection of HIV infection. We performed computational simulations on the mechanism of interaction between the nanoclusters and the streptavidin protein via in silico studies and showed that gold nanoclusters enhance the binding to the protein, by enhancing interaction between the Au atoms and the specific active site residues, compared to other metal nanoclusters. We also evaluated the role of glutathione conjugation in binding to gold nanoclusters with streptavidin. As proof of concept, GNCIA achieved a sensitivity limit of detection of HIV-1 p24 antigen in clinical specimens of 5 pg/ml, with a detection range up to1000 pg/ml in a linear dose-dependent manner. GNCIA demonstrated a threefold higher sensitivity and specificity compared to enzyme-linked immunosorbent assay for the detection of HIV p24 antigen. The specificity of the immunoassay was 100% when tested with plasma samples negative for HIV-1 p24 antigen and positive for viruses such as hepatitis B virus, hepatitis C virus, and dengue. GNCIA could be developed into a universal labeling technology using the relevant capture and detector antibodies for the specific detection of antigens of various pathogens in the future.
关键词： HIV infection,immunoassay,gold nanoclusters,early diagnosis,fluorescent sensors
更新于2025-09-10 09:29:36
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Environmental disinfection with photocatalyst as an adjunctive measure to control transmission of methicillin-resistant Staphylococcus aureus: a prospective cohort study in a high-incidence setting
摘要： Background: Environmental disinfection with continuously antimicrobial surfaces could offer superior control of surface bioburden. We sought to decide the efficacy of photocatalyst antimicrobial coating in reducing methicillin-resistant Staphylococcus aureus (MRSA) acquisition in high incidence setting. Methods: We performed prospective cohort study involving patients hospitalized in medical intensive care unit. A titanium dioxide-based photocatalyst was coated on high touch surfaces and walls. Five months of pre-intervention data were compared with five months of post-intervention data. The incidence rates of multidrug-resistant organism acquisition and the rates of hospital-acquired blood stream infection, pneumonia, urinary tract infection, and Clostridium difficile–associated diseases were compared using Cox proportional hazards regression analysis. Results: In total, 621 patients were included. There was significant decrease in MRSA acquisition rate after photocatalyst coating (hazard ratio, 0.37; 95% confidence interval, 0.14–0.99; p = 0.04). However, clinical identification of vancomycin-resistant Enterococcus spp. and multidrug-resistant Acinetobacter baumannii did not decrease significantly. The hazard of contracting hospital-acquired pneumonia during the intervention period compared to baseline period was 0.46 (95% confidence interval, 0.23–0.94; p = 0.03). Conclusions: In conclusion, MRSA rate was significantly reduced after photocatalyst coating. We provide evidence that photocatalyst disinfection can be an adjunctive measure to control MRSA acquisition in high-incidence settings. Trial registration: ISRCTN Registry (ISRCTN31972004). Registered retrospectively on November 19, 2018.
关键词： Environmental disinfection,Hospital-acquired infection,MRSA,Photocatalyst
更新于2025-09-09 09:28:46
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Automated Behavioral Analysis of Large <em>C. elegans</em> Populations Using a Wide Field-of-view Tracking Platform
摘要： Caenorhabditis elegans is a well-established animal model in biomedical research, widely employed in functional genomics and ageing studies. To assess the health and fitness of the animals under study, one typically relies on motility readouts, such as the measurement of the number of body bends or the speed of movement. These measurements usually involve manual counting, making it challenging to obtain good statistical significance, as time and labor constraints often limit the number of animals in each experiment to 25 or less. Since high statistical power is necessary to obtain reproducible results and limit false positive and negative results when weak phenotypic effects are investigated, efforts have recently been made to develop automated protocols focused on increasing the sensitivity of motility detection and multi-parametric behavioral profiling. In order to extend the limit of detection to the level needed to capture the small phenotypic changes that are often crucial in genetic studies and drug discovery, we describe here a technological development that enables the study of up to 5,000 individual animals simultaneously, increasing the statistical power of the measurements by about 1,000-fold compared to manual assays and about 100-fold compared to other available automated methods.
关键词： nematode library,neurodegeneration,amyloid formation,Alzheimer's disease,Drug discovery,Issue 141,phenotype-based screening,high-throughput screening,Immunology and Infection,C. elegans,large population analysis
更新于2025-09-09 09:28:46
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Label-Free Digital Holo-tomographic Microscopy Reveals Virus-Induced Cytopathic Effects in Live Cells
摘要： Cytopathic effects (CPEs) are a hallmark of infections. CPEs are difficult to observe due to phototoxicity from classical light microscopy. We report distinct patterns of virus infections in live cells using digital holo-tomographic microscopy (DHTM). DHTM is label-free and records the phase shift of low-energy light passing through the specimen on a transparent surface with minimal perturbation. DHTM measures the refractive index (RI) and computes the refractive index gradient (RIG), unveiling optical heterogeneity in cells. We find that vaccinia virus (VACV), herpes simplex virus (HSV), and rhinovirus (RV) infections progressively and distinctly increased RIG. VACV infection, but not HSV and RV infections, induced oscillations of cell volume, while all three viruses altered cytoplasmic membrane dynamics and induced apoptotic features akin to those caused by the chemical compound staurosporine. In sum, we introduce DHTM for quantitative label-free microscopy in infection research and uncover virus type-specific changes and CPE in living cells with minimal interference.
关键词： tomography,virus infection,apoptosis,herpes simplex virus,live-cell microscopy,membrane blebbing,cell volume,refractive index,cell contraction,rhinovirus,label-free microscopy,vaccinia virus
更新于2025-09-09 09:28:46
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Extended Release of an Anti–Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection
摘要： PURPOSE. To prolong the release of a heparan sulfate binding peptide, G2-C, using a commercially available contact lens as a delivery vehicle and to demonstrate the ability of the released peptide to block herpes simplex virus-1 (HSV-1) infection using in vitro, ex vivo, and in vivo models of corneal HSV-1 infection. METHODS. Commercially available contact lenses were immersed in peptide solution for 5 days prior to determining the release of the peptide at various time points. Cytotoxicity of the released samples was determined by MTT and cell cycle analysis, and the functional activity of the released samples were assessed by viral entry, and viral spread assay using human corneal epithelial cells (HCE). The ability to suppress infection in human and pig cornea ex vivo and mouse in vivo models were also assessed. RESULTS. Peptide G2-C was released through the contact lens. Following release for 3 days, the peptide showed significant activity by inhibiting HSV-1 viral entry and spread in HCE cells. Significant suppression of infection was also observed in the ex vivo and in vivo experiments involving corneas. CONCLUSIONS. Extended release of an anti–HS peptide through a commercially available contact lens can generate significant anti–HSV-1 activity and provides a new and effective way to control corneal herpes.
关键词： virus infection,corneal epithelium,peptide,contact lens
更新于2025-09-09 09:28:46
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Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
摘要： We describe here a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and machine learning. Identification of lymphocyte subtypes is important for the study of immunology as well as diagnosis and treatment of various diseases. Currently, standard methods for classifying lymphocyte types rely on labeling specific membrane proteins via antigen-antibody reactions. However, these labeling techniques carry the potential risks of altering cellular functions. The protocol described here overcomes these challenges by exploiting intrinsic optical contrasts measured by 3D quantitative phase imaging and a machine learning algorithm. Measurement of 3D refractive index (RI) tomograms of lymphocytes provides quantitative information about 3D morphology and phenotypes of individual cells. The biophysical parameters extracted from the measured 3D RI tomograms are then quantitatively analyzed with a machine learning algorithm, enabling label-free identification of lymphocyte types at a single-cell level. We measure the 3D RI tomograms of B, CD4+ T, and CD8+ T lymphocytes and identified their cell types with over 80% accuracy. In this protocol, we describe the detailed steps for lymphocyte isolation, 3D quantitative phase imaging, and machine learning for identifying lymphocyte types.
关键词： lymphocyte identification,machine learning,holotomography,immune cell,immunology,Immunology and Infection,Quantitative phase imaging,optical diffraction tomography,holographic microscopy,label-free imaging
更新于2025-09-04 15:30:14
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Acute subretinal abscess in <i>Staphylococcus aureus</i> septicaemia with endophthalmitis showcased by multimodal retinal imaging and with 2-year follow-up
摘要： A middle-aged man presented to emergency services with central vision loss in the setting of flu-like illness with fever. A striking subfoveal abscess was observed in the right fundus. Focal acute chorioretinal inflammation was noted in the asymptomatic fellow eye. Staphylococcus aureus septicaemia was subsequently diagnosed. He presented with undiagnosed HIV infection and latent syphilis. Serial high-definition multimodal retinal imaging showcased resolution of the dome-shaped subretinal abscess following treatment with intravenous flucloxacillin. A chorioretinal scar swiftly replaced the subfoveal abscess. Peripheral right vision and full left vision was retained. Vision loss due to endogenous endophthalmitis in systemic sepsis is an emergency requiring prompt multidisciplinary care. Sight and life are at risk—thus this is not a diagnosis to miss! Early recognition is paramount to health and in retaining vision. We briefly review relevant literature and portray how multimodal imaging guided response to treatment of acute subretinal abscess.
关键词： endophthalmitis,Staphylococcus aureus septicaemia,multimodal retinal imaging,HIV infection,acute subretinal abscess,latent syphilis
更新于2025-09-04 15:30:14