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Light-Induced Thickening of Photoreceptor Outer Segment Layer Detected by Ultra-High Resolution OCT Imaging
摘要： PURPOSE. We examined if light induces changes in the retinal structure that can be observed using optical coherence tomography (OCT). METHODS. Normal C57BL/6J mice (age 3–6 months) adapted to either room light (15 minutes to ~5 hours, 50–500 lux) or darkness (overnight) were imaged using a Bioptigen UHR-OCT system. Confocal histologic images were obtained from mice killed under light- or dark-adapted conditions. RESULTS. The OCT image of eyes adapted to room light exhibited signi?cant increases (6.1 6 0.8 lm, n ? 13) in total retina thickness compared to the same eyes after overnight dark adaptation. These light-adapted retinal thickness changes occurred mainly in the outer retina, with the development of a hypore?ective band between the RPE and photoreceptor-tip layers. Histologic analysis revealed a light-evoked elongation between the outer limiting membrane and Bruch’s membrane from 45.8 6 1.7 lm in the dark (n ? 5) to 52.1 6 3.7 lm (n ? 5) in the light. Light-adapted retinas showed an increase of actin staining in RPE apical microvilli at the same location as the hypore?ective band observed in OCT images. Elongation of the outer retina could be detected even with brief light exposures, increasing 2.1 6 0.3 lm after 15 minutes (n ? 9), and 4.1 6 1.0 lm after 2 hours (n ? 6). Conversely, dark-adaptation caused outer retinal shortening of 1.4 6 0.4 lm (n ? 7) and 3.0 6 0.5 lm (n ? 8) after 15 minutes and 2 hours, respectively. CONCLUSIONS. Light-adaption induces an increase in the thickness of the outer retina and the appearance of a hypore?ective band in the OCT image. This is consistent with previous reports of light-induced ?uid accumulation in the subretinal space.
关键词： optical coherence tomography,subretinal space,retinal pigment epithelium,light/dark adaptation,outer segments
更新于2025-09-10 09:29:36
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Macular Pigment and Visual Performance in Low-Light Conditions
摘要： By reducing rod intrusion and improving efficiency of neural signaling throughout the visual system, macular pigment (MP) could improve many aspects of visual performance in low-light level conditions. Our study examined this possibility for a variety of visual performance parameters, including spatial resolution, dark adaptation kinetics, and color detection.
关键词： light/dark adaptation,macular pigment,lutein,visual acuity,visual performance
更新于2025-09-04 15:30:14