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High-Resolution Label-Free Studies of Molecular Distribution and Orientation in Ultrathin, Multicomponent Model Membranes with Infrared Nano-Spectroscopy AFM-IR
摘要： Biological membranes are undoubtedly very interesting systems. Unfortunately, their analysis is very complicated and therefore simplified artificial models, like Langmuir monolayers, are frequently applied. In this work a novel method of label-free monomolecular films analysis based on infrared nano-spectroscopy (AFM-IR) is presented. In order to verify applicability of this approach well-defined referential system of sphingomyelin (SM), cholesterol (Chol) and cyclosporin A (CsA) is applied. AFM-IR method allows to directly and chemoselectively map the distribution of components in model lipid membranes. Additionally the orientation of Chol and SM molecules in the monolayer is determined by application of two perpendicular infrared laser polarizations. This paper is the first report of using AFM-IR to analyze LB films with extremely high sensitivity.
关键词： phase separation,lipid rafts,Langmuir-Blodgett films,Cyclosporin A,AFM-IR
更新于2025-09-19 17:15:36
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Self‐assembly of lipid rafts revealed by fluorescence correlation spectroscopy in living breast cancer cells
摘要： In the study, imaging this polarisation Lipids and proteins in the plasma membrane are laterally heterogeneous and formalised as lipid rafts featuring unique biophysical properties. However, self-assembly mechanism of lipid raft cannot be revealed even its physical properties and components were determined in specific physiological two-photon processes. generalised and fluorescence correlation spectroscopy were used to study the fusion of lipid rafts through the membrane phase and the lateral diffusion of lipids in living breast cancer cells. A self-assembly model of lipid rafts and lipid associated with to phase was membrane demonstrate the lipid sorting ability of lipid rafts in the plasma membrane. The results showed that the increased proportion of slow subdiffusion of GM1-binding cholera toxin B-subunit (CT-B) is accompanied with an increased liquid-ordered domain during the β-estradiol-induced fusion of lipid rafts. And slow subdiffusion of CT-B was vanished with the depletion of lipid rafts. Whereas the dialkylindocarbocyanine (DiIC18) diffusion was not specifically regulated by lipid rafts. This study will open up a new insight for uncovering the self-assembly of lipid rafts in specific pathophysiological processes.
关键词： Lipid rafts,Self-assembly,Membrane phase,Fluorescence correlation spectroscopy,Anomalous diffusion
更新于2025-09-11 14:15:04