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A selective diaminomaleonitrile-based dual channel emissive probe for Al3+ and its application in living cell imaging
摘要： A selective diaminomaleonitrile-based fluorescent probe for Al3+(L) has been synthesized and characterized. The sensing behavior of L towards different metal ions was investigated by fluorescent spectra and competition experiment. The results showed that L exhibited a dual channel emission (λem 522 & 558 nm) with yellow fluorescence in the presence of Al3+ over other metal ions in EtOH solution. The probe L was bound to Al3+ in a 1: 2 stoichiometric manner as supported by Job’s plot analysis. The binding constant was 9.55×108 M-2 with the detection limit of 10.3 nM. The turn-on response was attributed to the inhibition of PET and C=N isomerization after binding with Al3+ while the dual emissions were due to ESIPT and ICT processes. In addition, the application of L for imaging Al3+ in living Hela cells was achieved.
关键词： diaminomaleonitrile-based,Al3+,living cell imaging,selective,dual channel
更新于2025-09-23 15:22:29
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Bridge between Temperature and Light: Bottom-Up Synthetic Route to Structure-Defined Graphene Quantum Dots as a Temperature Probe In Vitro and in Cells
摘要： Owing to their unique superiorities in chemical and photoluminescence (PL) stability, low toxicity, biocompatibility, and easy functionalization, graphene quantum dots (GQDs) were widely used in cell imaging, probes, and sensors. However, further development and deeper research of GQDs were restricted by their imprecise and complex structure and accompanying controversial PL mechanism. In this work, two kinds of structure defined water-soluble GQDs, with different oxidation degree, were synthesized from molecules by using bottom-up syntheses methods. After studied by a serial of characterizations, their optical properties, functional groups, molecular weight, and structural information were obtained. The optical properties of GQDs could be optimized by controlling their oxidation degree. PL mechanism of GQDs was investigated by comparing their structure and properties. Furthermore, robust, stable, and precise temperature probes were designed by using the GQDs, which exhibited an excellent wide responding range, which covered the whole physiology temperature range, from 0 ℃ to 60 ℃ in water. Moreover, the GQDs were successfully applied as temperature responsive fluorescence probe in Hela cell line. These works put forward a solid foundation for the applications of biological thermo probes and selectively temperature detectors in vitro cellular and in vivo.
关键词： structure controllable,living cell imaging,temperature probe,bottom-up synthesis,mechanism,graphene quantum dots
更新于2025-09-23 15:19:57
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Construction of acetaldehyde-modified g-C3N4 ultrathin nanosheets via ethylene glycol-assisted liquid exfoliation for selective fluorescence sensing of Ag+
摘要： We successfully prepared acetaldehyde-modified g-C3N4 ultrathin nanosheets (ACNNSs) by a simple ethylene glycol-assisted liquid exfoliation method. The introduction of acetaldehyde regulated the surface energy of g-C3N4 to better match with that of water, which improved the exfoliation efficiency. Moreover, the acetaldehyde introduces defects into the g-C3N4 structure, which can act as excitation energy traps and cause considerable variation in the fluorescence emission. Benefiting from the stable photoluminescence (PL) emission, good water solubility and biocompatibility, the obtained ACNNSs showed a selective fluorescent response to Ag+ both in aqueous solution and living cells. The strong absorption and intimate contact with Ag+, and its appropriate redox potential of ACNNSs contributed to this excellent fluorescent response. A simple and environmental friendly approach was proposed to simultaneously achieve modification and exfoliation of g-C3N4 in aqueous solution. These findings might lead to wider applications of carbon-based nanomaterials as active materials for fluorescence detection in the environment.
关键词： Graphitic carbon nitride nanosheets,Living-cell imaging,Glycol-assisted exfoliation,Fluorescent sensor,Acetaldehyde-modified,Ag+
更新于2025-09-10 09:29:36