- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Microscopy of the Heart || Optical Sectioning Microscopy at ‘Temporal Super-Resolution’
摘要： Within the recent years several super-resolution microscopic methods were developed, where the super-resolution refers to bringing the optical resolution beyond the diffraction limit introduced by Ernst Abbe, which was believed to be a real limit for quite some time. The popularity of the method also in cardiac related research can be followed in the chapter ‘Quantitative super-resolution microscopy of cardiac myocytes’ in this book. In parallel to this spatial super-resolution progress, within the past two decades there was a dynamic development of high speed–high resolution imaging initially towards video-rate (30 frames per second, also referred to as ‘real time’-imaging) but soon to ever increasing frame rates reaching the kHz order of magnitude these days. Many processes, especially those in excitable cells such as neurons and cardiomyocytes [1] or cells in ?ow like erythrocytes or leukocytes [2], require even higher temporal resolution to elucidate the kinetics of processes like the Excitation-Contraction Coupling (ECC). Such ultra high speed recordings still require a diffraction limited spatial resolution to correlate function and subcellular structures [3]. Within this chapter we review optical sectioning microscopy and their application in cellular cardiology. In this approach we focus on methods that allow to access any part of the cell, i.e. we exclude methods that are intrinsically limited to surface investigations like total internal re?ection ?uorescence (TIRF) microscopy [4] or scanning near ?eld optical microscopy (SNOM) [5]. In similarity we exclude techniques that require several images to calculate an image section such as deconvolution microscopy [6] or structured illumination microscopy [7] (e.g., Apotome.2, Zeiss, Jena, Germany).
关键词： super-resolution microscopy,Excitation-Contraction Coupling,high-speed imaging,cardiomyocytes,optical sectioning
更新于2025-09-23 15:19:57
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Deep learning optical-sectioning method
摘要： Current optical-sectioning methods require complex optical system or considerable computation time to improve imaging quality. Here we propose a deep learning-based method for optical sectioning of wide-field images. This method only needs one pair of contrast images for training to facilitate reconstruction of an optically sectioned image. The removal effect of background information and resolution that is achievable with our technique is similar to traditional optical-sectioning methods, but offers lower noise levels and a higher imaging depth. Moreover, reconstruction speed can be optimized to 14 Hz. This cost-effective and convenient method enables high-throughput optical sectioning techniques to be developed.
关键词： wide-field imaging,optical sectioning,deep learning,convolutional neural network,background suppression
更新于2025-09-10 09:29:36
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Optical sectioning with a Wiener-like filter in Fourier integral imaging microscopy
摘要： Non-scanning, single-shot, 3D integral microscopy with optical sectioning is presented. The method is based on the combination of Fourier-mode integral microscopy with a 3D deconvolution technique. Specifically, the refocused volume provided by a regular back-projection algorithm is 3D deconvolved with a synthetic 3D impulse response function that takes into account the number and positions of the elemental images. The use of this hybrid technique provides a stack of true-color depth-refocused images with significant gain of optical sectioning. The stack can be used, among other applications, to inspect inside the thick microscope specimen, to calculate collections of perspective views with fine angular resolution and extended full parallax, and also to display 3D images in an integral monitor. The method here presented is validated with both simulation and experimental data.
关键词： Fourier-mode integral microscopy,optical sectioning,3D integral microscopy,3D deconvolution,synthetic 3D impulse response function
更新于2025-09-10 09:29:36
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Optical sectioning in multifoci Raman hyperspectral imaging
摘要： In this study, we compared the depth discrimination and speed performance of multifoci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator was employed for the generation of multifoci laser beams, and a digital micromirror device was used as a software‐configurable reflective pinhole array. The patterns of the laser foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance‐to‐size ratios were tested and compared. After optimization of the laser‐foci pattern, we demonstrated the feasibility of multifoci Raman hyperspectral microscopy for recording depth‐resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth discrimination and short acquisition times (20 min for single plane confocal image) were achieved.
关键词： single cell imaging,cross‐talk,confocal Raman imaging,multi‐beam,optical sectioning
更新于2025-09-04 15:30:14