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Phage-based assay for rapid detection of bacterial pathogens in blood by Raman spectroscopy
摘要： Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. In this study, from random M13 phage display libraries, we selected phage clones that specifically bind surface of Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli. Then, commercial magnetic beads were functionalized with phage clones through covalent bond and used as capture and concentrating of pathogens from blood. We found that phage-magnetic beads complex represents a network which enables a cheap, high sensitive and specific detection of the bacteria involved in sepsis by micro-Raman spectroscopy. The enter process required 6 h and has the limit of detection of 10 Colony Forming Units on 7 ml of blood (CFU/7 ml).
关键词： Phage-capture system,Raman spectroscopy,M13 filamentous phage,Sepsis,Phage display
更新于2025-09-23 15:23:52
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Is Greenlight laser photoselective vaporization of the prostate a safe technique? Results from a wide monocentric series complications analysis, with a focus on postoperative fever and sepsis
摘要： GreenLight laser Photoselective Vaporization of the Prostate (PVP) is an effective surgical treatment for Benign Prostatic Hyperplasia (BPH). Moreover, its high coagulative power makes GreenLight reliable in high-risk patients (pts). Despite this, post-operative complications still represent a source of concern when surgery is performed in the most fragile pts. The aim of our study was to retrospectively review a large cohort of pts underwent GreenLight PVP and perform a complications analysis, with a focus on post-operative fever and sepsis.
关键词： sepsis,Benign Prostatic Hyperplasia,postoperative complications,Photoselective Vaporization of the Prostate,GreenLight laser,fever
更新于2025-09-19 17:13:59
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Rapid and Digital Detection of Inflammatory Biomarkers Enabled by a Novel Portable Nanoplasmonic Imager
摘要： New point-of-care diagnostic devices are urgently needed for rapid and accurate diagnosis, particularly in the management of life-threatening infections and sepsis, where immediate treatment is key. Sepsis is a critical condition caused by systemic response to infection, with chances of survival drastically decreasing every hour. A novel portable biosensor based on nanoparticle-enhanced digital plasmonic imaging is reported for rapid and sensitive detection of two sepsis-related inflammatory biomarkers, procalcitonin (PCT) and C-reactive protein (CRP) directly from blood serum. The device achieves outstanding limit of detection of 21.3 pg mL?1 for PCT and 36 pg mL?1 for CRP, and dynamic range of at least three orders of magnitude. The portable device is deployed at Vall d’Hebron University Hospital in Spain and tested with a wide range of patient samples with sepsis, noninfectious systemic inflammatory response syndrome (SIRS), and healthy subjects. The results are validated against ultimate clinical diagnosis and currently used immunoassays, and show that the device provides accurate and robust performance equivalent to gold-standard laboratory tests. Importantly, the plasmonic imager can enable identification of PCT levels typical of sepsis and SIRS patients in less than 15 min. The compact and low-cost device is a promising solution for assisting rapid and accurate on-site sepsis diagnosis.
关键词： imaging biosensors,nanoplasmonics,point-of-care diagnostics,sepsis,gold nanoparticles
更新于2025-09-12 10:27:22
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Photoacoustic microscopy for evaluating a lipopolysaccharide-induced inflammation model in mice
摘要： Photoacoustic microscopy (PAM) is a noninvasive imaging technique and is excellent to study structural and functional changes in the microcirculation. In this work, a lipopolysaccharide (LPS)-induced inflammation model in mice is noninvasively evaluated by PAM. PAM is used to image the microvascular structural changes in mice for 8 hours after the LPS with different concentrations is applied. Quantitative analysis of five vessel parameters is conducted, which shows that the rate of reduction in microvasculature is highly dependent on the applied LPS concentrations. For low-concentration LPS, changes in the microvasculature are not obvious over the observation period, while for high-concentration LPS, quick and marked reduction in the microvasculature is observed. In addition, changes in capillaries are more significant than those in relatively large vessels. The results show that PAM is able to evaluate the inflammation mouse model by studying structural (and potentially functional) changes in the microcirculation. Further, PAM may have potential for early intervention and treatment plan optimization of sepsis by monitoring the microcirculation and inflammatory response.
关键词： inflammatory response,photoacoustic imaging,photoacoustic microscopy,sepsis,microcirculation
更新于2025-09-10 09:29:36
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Label-free bacteria quantification in blood plasma by a bioprinted microarray based interferometric point-of-care device
摘要： Existing clinical methods for bacteria detection lack in speed, sensitivity and importantly in Point-of-Care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Aiming that, we here report a portable PoC device based on Lens-free Interferometric Microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample?to?data turnaround time of 40 minutes. Our technology features detection sensitivity of a single bacterial cell both in buffer and diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and non-infectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality.
关键词： microarray,label-free detection,plasma samples,nanoplasmonics,sepsis,bacteria
更新于2025-09-09 09:28:46