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Simultaneous species and sex identification of silkworm pupae using hyperspectral imaging technology
摘要： To obtain high-quality raw silk and improve the economic values of sericulture industry, sex needs to be discriminated first before cross-breeding. Much work has been reported about sex identification. However, to realize automatic separation of silkworm pupae, the species also needs to be classified, which no research has ever explored. Hence, this paper studied the feasibility of visible and near-infrared hyperspectral imaging technology to identify the species and sex of silkworm pupae. 288 hyperspectral images of silkworm pupae were collected and the average spectra were extracted from the region of interest, around the tail region of silkworm pupae. Successive projection algorithm was served as a variable selection method to choose the optimal wavelengths from the full spectra. At the same time, principal component analysis was used to choose the characteristic images. Then, the gray-level co-occurrence matrix was implemented on the first three principal component images (accounted for 99.05% of the total variances) to extract 48 textural features. Partial least squares discriminant analysis and support vector machine models were built, respectively, based on the spectral data, textural data and fusion data that included spectral and textural data, in which the support vector machine model based on the fusion data, gave the best species and sex identification result with an accuracy of 95.83%. It demonstrated that the hyperspectral imaging technology could be a new and nondestructive method to replace the manual work.
关键词： silkworm pupa,species,identification sex,Hyperspectral imaging
更新于2025-09-09 09:28:46
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Accurate Identification of the Sex and Species of Silkworm Pupae Using Near Infrared Spectroscopy
摘要： The present study proposes a novel method to discriminate the sex and species of silkworm pupae using NIR spectroscopy (800–2778 nm). The spectra from 840 silkworm pupae were collected then divided into a calibration set (700) and a test set (140) using the Kennard–Stone (KS) algorithm. The recognition models were built using the radial basis function and neural network (RBF–NN) and support vector machine (SVM) approaches. The species and sex identi?cation results using the RBF–NN and SVM models based on full spectral data achieved a low accuracy of 5% and 33.57%, respectively. To improve the accuracy and decrease the processing time, both principal component analysis (PCA) and linear discriminant analysis (LDA) were used to reduce the data dimensions. The performance of the optimized SVM model (92.14%) was much better than the RBF–NN model (19.29%) based on PCA. Overall, the best discrimination results were obtained using the RBF–NN and SVM models based on LDA, providing an accuracy of 100%. These promising results have shown that the LDA–SVM and LDA–RBF–NN models can accurately recognize the sex and species of silkworm pupae using NIR spectroscopy.
关键词： silkworm pupa,species,NIR spectroscopy,sex
更新于2025-09-09 09:28:46