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<p>A multifunctional-targeted nanoagent for dual-mode image-guided therapeutic effects on ovarian cancer cells</p>
摘要： Nanomedicine has emerged as a novel therapeutic modality for cancer treatment and diagnosis. Lipid–polymer hybrid nanoparticles (LPHNPs) are core–shell nanoparticle (NP) structures comprising polymer cores and lipid shells, which exhibit complementary characteristics of both polymeric NPs and liposomes. However, it is difficult to wrap perfluoropentane (PFP) into core–shell NPs in the existing preparation process, which limits its application in the integration of diagnosis and treatment. Methods: The folate-targeted LPHNPs-loaded indocyanine green/PFP-carrying oxygen (TOI_HNPs) using a combination of two-step method and solution evaporation technique for the first time. The essential properties and dual-mode imaging characteristics of developed NPs were determined. The cellular uptake of TOI_HNPs was detected by confocal microscopy and flow cytometry. The SKOV3 cell viability and apoptosis rate were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry. The ROS was demonstrated by fluorescence microplate reader and the expression of hypoxia-inducible factor 1-alpha (HIF-1α) and IL-6 was detected by Western blot. Results: TOI_HNPs showed spherical morphology with particle size about (166.83±5.54) nm and zeta potential at -(30.57±1.36) mV. It exhibited better stability than lipid NPs and higher encapsulation efficiency as well as active targeting ability than poly (lactic-co-glycolic acid) (PLGA) NPs. In addition, the novel NPs could also act as the contrast agents for ultrasound and photoacoustic imaging, providing precision guidance and monitoring. Furthermore, TOI_HNPs-mediated photo–sonodynamic therapy (PSDT) caused more serious cell damage and more obvious cell apoptosis, compared with other groups. The PSDT mediated by TOI_HNPs induced generation of intracellular ROS and downregulated the expression of HIF-1α and IL-6 in SKOV3 cells. Conclusion: This kind of multifunctional-targeted nanoagent may provide an ideal strategy for combination of high therapeutic efficacy and dual-mode imaging guidance.
关键词： core-shell nanoparticle,ultrasound,photo-sonodynamic therapy,phase transformation,photoacoustic imaging,laser
更新于2025-11-14 17:03:37
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Crystalline Semiconductor Boron Quantum Dots
摘要： Zero-dimensional boron structures have always been the focus of theoretical research owing to its abundant phase structures and special properties. Boron clusters have been reported extensively by combining structure searching theories and photoelectron spectroscopy experiments, however, crystalline boron quantum dots (BQDs) have rarely been reported. Here we report the preparation of large-scale and uniform crystalline semiconductor BQDs from the expanded bulk boron powders via a facile and efficient probe ultrasonic approach in acetonitrile solution. The obtained BQDs have 2.46 nm in an average lateral size and 2.81 nm in thickness. Optical measurements demonstrate that strong quantum confinement effect occurs in the BQDs, implying the increase of the bandgap from 1.80 eV for the corresponding bulk to 2.46 eV for the BQDs. By injecting the BQDs into polyvinylpyrrolidone as an active layer, a BQDs-based memory device is fabricated which shows a rewriteable nonvolatile memory effect with a low transition voltage of down to 0.5 V and a high ON/OFF switching ratio of 103 as well as a good stability.
关键词： ultrasound,quantum dots,nonvolatile memory device,quantum confinement effect,boron
更新于2025-11-14 15:23:50
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Targeted delivery of reduced graphene oxide nanosheets using multifunctional ultrasound nanobubbles for visualization and enhanced photothermal therapy
摘要： Ultrasound molecular imaging as a promising strategy, which involved the use of molecularly targeted contrast agents, combined the advantages of contrast-enhanced ultrasound with the photothermal effect of reduced graphene oxide (rGO). Methods and results: The heparin sulfate proteoglycan glypican-3 (GPC3) is a potential molecular target for hepatocellular carcinoma (HCC). In this study, we covalently linked biotinylated GPC3 antibody to PEGylated nano-rGO to obtain GPC3-modified rGO-PEG (rGO-GPC3), and then combined rGO-GPC3 with avidinylated nanobubbles (NBs) using biotin-avidin system to prepare NBs-GPC3-rGO with photothermal effect and dispersibility, solubility in physiological environment. The average size of NBs-GPC3-rGO complex was 700.4±52.9 nm due to the polymerization of biotin-avidin system. Scanning electron microscope (SEM) showed NBs-GPC3-rGO attached to human hepatocellular carcinoma HepG2 cell. The ultrasound-targeted nanobubble destruction (UTND) technology make use of the physical energy of ultrasound exposure for the improvement of rGO delivery. Compared with other control groups, the highest nanobubble destruction efficiency of NBs-GPC3-rGO was attributed to the dissection effect of rGO on UTND. This is a positive feedback effect that leads to an increase in the concentration of rGO around the HepG2 cell. So NBs-GPC3-rGO using UTND and near-infrared (NIR) irradiation resulted in cell viability within 24 h, 48 h, 72 h lower than other treatment groups. Conclusion: This work established NBs-GPC3-rGO as an ultrasonic photothermal agent due to its suitable size, imaging capability, photothermal efficiency for visual photothermal therapy in vitro.
关键词： ultrasound-targeted nanobubble destruction,photothermal therapy,glypican-3,reduced graphene oxide,HepG2 cell
更新于2025-09-23 15:23:52
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[IEEE 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - Krakow, Poland (2018.10.16-2018.10.18)] 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - Pre-operative AR-ultrasound application for determination of individual hip posture
摘要： The application developed is using Augmented Reality technique for pre-determine a patients individual pelvic posture. The Measurements were done under use of a hip model and a tracked ultrasound probe which was first covered with a 2D trackable marker and then coated with a water transfer printing technique. Three landmarks were needed for calculation of the pelvic posture. Preoperative examination of pelvic rotation could allow individualized placement of the acetabular cup for each hip patient, thereby reducing dislocation rates and impingement on the implant. Through interactive AR visualization the user could point the depth profile of the ultrasound image with a virtual pointer. For both trackable targets the pelvic posture was measured with a webcam and a smartphone camera. The trials were evaluated among themselves and on the basis of their repeatability and accuracy.
关键词： preoperative examination,pelvic posture,augmented reality,ultrasound
更新于2025-09-23 15:23:52
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[IEEE 2018 19th International Conference of Young Specialists on Micro/Nanotechnologies and Electron Devices (EDM) - Erlagol (Altai Republic), Russia (2018.6.29-2018.7.3)] 2018 19th International Conference of Young Specialists on Micro/Nanotechnologies and Electron Devices (EDM) - The System of Frequency Matching of the Ultrasonic Generators with the Radiators in “Bulava” Apparatuses
摘要： The article is devoted to the problem concerned with the determination of resonance frequency of the ultrasonic vibrating systems (the radiators) intended for the operation as a part of the ultrasonic technological apparatuses of “Bulava” type.
关键词： frequency control system,generator,resonance matching,Ultrasound
更新于2025-09-23 15:23:52
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Characterization of Placental Microvasculature Using Superb Microvascular Imaging
摘要： Superb Microvascular Imaging (SMI; Canon Medical Systems, Tustin, CA) uses clutter suppression to extract flow signals at rapid frame rates, which provides high-resolution vessel-branching details without the need for contrast agents. The potential diagnostic benefits of SMI, as described in other areas of medicine, requires further exploration during pregnancy. In this pictorial essay, we demonstrate the complementary use of SMI compared to conventional Doppler ultrasound and how it may improve our ability to characterize placental microvascular patterns without the need for ultrasound contrast agents.
关键词： obstetrics,placenta accreta,chorioangioma,Doppler ultrasound,morbidly adherent placenta
更新于2025-09-23 15:23:52
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Breast tumor classification using different features of quantitative ultrasound parametric images
摘要： Rationale and objectives The ultrasound B-mode-based morphological and texture analysis and Nakagami parametric imaging have been proposed to characterize breast tumors. Since these three feature categories of ultrasonic tissue characterization supply information on different physical characteristics of breast tumors, by combining the above methods is expected to provide more clues for classifying breast tumors. Materials and methods To verify the validity of the concept, raw data were obtained from 160 clinical cases. Six different types of morphological-feature parameters, four texture features, and the Nakagami parameter of benignancy and malignancy were extracted for evaluation. The Pearson’s correlation matrix was used to calculate the correlation between different feature parameters. The fuzzy c-means clustering and stepwise regression techniques were utilized to determine the optimal feature set, respectively. The logistic regression, receiver operating characteristic curve, and support vector machine were used to estimate the diagnostic ability. Results The best performance was obtained by combining morphological-feature parameter (e.g., standard deviation of the shortest distance), texture feature (e.g., variance), and the Nakagami parameter, with an accuracy of 89.4%, a specificity of 86.3%, a sensitivity of 92.5%, and an area under receiver operating characteristic curve of 0.96. There was no significant difference between using fuzzy c-means clustering, logistic regression, and support vector machine based on the optimal feature set for breast tumors classification. Conclusion Therefore, we verified that different physical ultrasonic features are functionally complementary and thus improve the performance in diagnosing breast tumors. Moreover, the optimal feature set had the maximum discriminating performance should be irrelative to the power of classifiers.
关键词： Morphological features,Breast ultrasound,Nakagami parameter,Texture features,Classification
更新于2025-09-23 15:23:52
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Effects of carbon nanotube thermal conductivity on optoacoustic transducer performance
摘要： CNT-PDMS composite is regarded as a promising candidate for optoacoustic transducer application. However, the relationship between the CNT thermal conductivity and optoacoustic transducer performance is still unde?ned. To explore this relationship, four types of CNTs with different diameters and lengths were employed to successfully fabricate four types of optoacoustic transducers. The thermal properties of CNTs were analyzed and discussed in this paper; the laser-generated ultrasound and optoacoustic conversion ef?ciency of the transducers were experimentally and theoretically investigated. Results of comparison revealed the CNT with a diameter and length of 8 nm and 10e30 mm, respectively, to have the highest thermal conductivity. With this type of CNT/PDMS composite, the optoacoustic conversion ef?ciency reached as high as 9.59 (cid:1) 10-3. Overall, the results demonstrate that higher CNT thermal conductivity corresponds to better optoacoustic transducer performance.
关键词： Laser-generated ultrasound,Thermal conductivity,Optoacoustic transducer,Optoacoustic conversion ef?ciency,CNT
更新于2025-09-23 15:23:52
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Comparison of different non-invasive diagnostic techniques used for HMME-PDT in the treatment of port wine stains
摘要： Background: Hemoporfin-mediated photodynamic therapy (HMME-PDT) is an effective method for treating port wine stains (PWS). While, it still lacks methods to evaluate the treatment of HMME-PDT for PWS effectively and objectively. Objective: This study aimed to compare the value of different non-invasive diagnostic techniques used for HMME-PDT treatment evaluation in PWS. Methods: Thirty-one lesions of 22 patients with PWS were treated with HMME-PDT. Four non-invasive diagnostic techniques including VISIA-CRTM system, dermoscopy, high-frequency ultrasound (HFUS) and laser speckle imager (LSCI) were used to obtain standard radiographic data containing skin color, skin thickness, blood vessel morphology, blood vessel distribution and blood perfusion from lesions and normal skin surrounded before and after HMME-PDT. Results: The standard image pattern of VISIA-CRTM system showed color change of the lesions of PWS after HMME-PDT. RBX-red image of VISIA-CRTM system showed that erythema was highly aggregated even on invisible lesions at baseline but decreased after HMME-PDT. The erythema index reduced value d was related to the efficacy rating (γ=0.631, P＜0.05). Dermoscopy showed that the number of spot-like and irregular linear vessels increased correlated with increase of clinical classification. After HMME-PDT, vascular rupture was observed by dermoscopy. The response rate of lesions with vascular rupture was 100.00% (20/20). And the response rate of lesions without vascular rupture was 63.64% (7/11). Vascular rupture sign was correlated with better efficacy (P＜0.05). High-frequency ultrasound showed that dermis of PWS thickened and arranged loosely with scattered linear hypoechoic signal. After HMME-PDT, the dermal layer of the lesions became thinner with the linear hypoechoic signal decreased. The response rate of lesions with linear hypoechoic signal was 76.92% (10/13), and that without linear hypoechoic signal was 94.44% (17/18). The lesions without linear hypoechoic signal in dermis showed better efficacy (P＜0.05). In some lesions, Laser speckle contrast showed high blood perfusion signal in PWS lesions and a blood perfusion reduction after HMME-PDT. Conclusion: VISIA-CRTM system can be used to observe not only visible but also invisible lesions of PWS. Moreover, lesions fading after HMME-PDT treatment can be described objectively by VISIA-CRTM system. Dermoscopy played an important role in the clinical classification of PWS, including assessing vascular injury after HMME-PDT, guiding the adjustment of therapeutic dose, and selecting the end point of treatment. HFUS and LSCI can be both used to assist the treatment response evaluation of HMME-PDT.
关键词： dermoscopy,port-wine stains,laser speckle contrast imaging,hemoporfin-mediated photodynamic therapy,VISIA-CRTM system,high-frequency ultrasound
更新于2025-09-23 15:23:52
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Clinical Value of Positron Emission Tomography-Computed Tomography Combined with Ultrasound in Detection of Primary Tumors in Patients with Malignant Ascites
摘要： Objective: To investigate and discuss the clinical value of positron emission tomography-computed tomography (PET-CT) combined with ultrasound in detection of primary tumors in patients with malignant ascites (MA). Materials and Methods: A total of 122 malignant tumor patients with ascites as the initial symptom and 48 patients with benign ascites were enrolled in this study. All patients underwent PET-CT and abdominal B-ultrasound examinations. The corresponding specificity, sensitivity, accuracy rate, positive predictive value, and negative predictive value of PET-CT, abdominal B-ultrasound, and combined detection group were recorded, respectively, with pathological findings as the gold standards. Statistical Product and Service Solutions 17.0 software was used for statistical analysis. p < 0.05 suggested that the difference was statistically significant. Results: The detection rate of primary foci through PET-CT was 79.5%, of which the detection rate of primary foci of MA derived from gastric cancer was the highest. The detection rate of primary foci through B-ultrasound was 62.5%, which is the highest for MA derived from ovarian cancer. B-ultrasound had the highest specificity in diagnosing the primary foci of MA (73.2%), PET-CT had the highest sensitivity in diagnosing the primary foci of MA (91.7%), and PET-CT combined with abdominal B-ultrasound had the highest sensitivity and accuracy in diagnosing the primary foci of MA (98.1% and 89.1%, respectively). The diagnostic accuracy rate of B-ultrasound was the highest in detecting tumors >5 cm (77.0%), whereas that of PET-CT was the highest in detecting tumors of 3–5 cm (84.2%). Conclusion: The PET-CT combined with ultrasound is conducive to improving the diagnostic efficiency for primary tumors in patients with MA.
关键词： ultrasound,malignant ascites,PET-CT,clinical value
更新于2025-09-23 15:23:52